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Mx1 of black carp functions importantly in the antiviral innate immune response
Xiao, Jun1; Yan, Jun1; Chen, Hui1; Li, Jun1; Tian, Yu1,2; Tang, Luosheng2; Feng, Hao1,3
2016-11-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume58Issue:1Pages:584-592
Abstract

Mx (myxovirus resistance) is an important antiviral protein in the innate immune responses of vertebrates to microbial pathogens. In this study, we cloned and characterized Mx1 of black carp (Mylopharyngodon piceus). The full-length cDNA of black carp Mx1 (bcMx1) consists of 2781 nucleotides and the predicted bcMx1 protein contains 631 amino acids. bcMx1 contains a GTPase domain at the N-terminnus, a "central interactive domain" in the middle and a GTPase effector domain at the C-terminus. bcMx1 mRNA was constitutively transcribed in all tissues tested, including the heart, liver, spleen, kidney, intestine, muscle, skin and gill; and bcMx1 mRNA levels increased in all but the gill after grass carp reovirus (GCRV) or viraemia of carp virus (SVCV) infection. Quantitative PCR analysis of Mylopharyngodon piceus fin (MPF) cells indicated that bcMx1 mRNA levels increased after GCRV or SVCV infection at different multiplicities of infection (MOI). Western blotting demonstrated that the molecular weight of bcMx1 is similar to 75 kDa and immunofluorescent staining data of both HeLa cells and EPC cells showed that bcMx1 is a cytosolic protein. EPC cells transfected with plasmid expressing bcMx1 showed increased antiviral activity against SVCV and GCRV. All our data suggest that bcMx1 is an antiviral protein in the innate immune response of the black carp. (C) 2016 Elsevier Ltd. All rights reserved.

KeywordBlack carp Mx Innate immunity
DOI10.1016/j.fsi.2016.10.002
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31272634] ; National Natural Science Foundation of China[81471963] ; National Natural Science Foundation of China[31502159] ; Fund of FEBL[2013FB02] ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province[20134486]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000387523100068
WOS KeywordPANCREATIC NECROSIS VIRUS ; PATTERN-RECOGNITION RECEPTORS ; INTERFERON-STIMULATED GENES ; SEABREAM SPARUS-AURATA ; IKK-EPSILON GENE ; INFLUENZA-VIRUS ; MYLOPHARYNGODON-PICEUS ; PATHOGEN RECOGNITION ; VIRAL-INFECTIONS ; RESISTANCE
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/34105
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorTang, Luosheng; Feng, Hao
Affiliation1.Hunan Normal Univ, Coll Life Sci, Minist Educ China, Key Lab Prot Chem & Dev Biol, Changsha 410081, Hunan, Peoples R China
2.Cent S Univ, Xiangya Hosp 2, Changsha 410011, Hunan, Peoples R China
3.State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Xiao, Jun,Yan, Jun,Chen, Hui,et al. Mx1 of black carp functions importantly in the antiviral innate immune response[J]. FISH & SHELLFISH IMMUNOLOGY,2016,58(1):584-592.
APA Xiao, Jun.,Yan, Jun.,Chen, Hui.,Li, Jun.,Tian, Yu.,...&Feng, Hao.(2016).Mx1 of black carp functions importantly in the antiviral innate immune response.FISH & SHELLFISH IMMUNOLOGY,58(1),584-592.
MLA Xiao, Jun,et al."Mx1 of black carp functions importantly in the antiviral innate immune response".FISH & SHELLFISH IMMUNOLOGY 58.1(2016):584-592.
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