Mx1 of black carp functions importantly in the antiviral innate immune response | |
Xiao, Jun1; Yan, Jun1; Chen, Hui1; Li, Jun1; Tian, Yu1,2; Tang, Luosheng2; Feng, Hao1,3 | |
2016-11-01 | |
Source Publication | FISH & SHELLFISH IMMUNOLOGY
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ISSN | 1050-4648 |
Volume | 58Issue:1Pages:584-592 |
Abstract | Mx (myxovirus resistance) is an important antiviral protein in the innate immune responses of vertebrates to microbial pathogens. In this study, we cloned and characterized Mx1 of black carp (Mylopharyngodon piceus). The full-length cDNA of black carp Mx1 (bcMx1) consists of 2781 nucleotides and the predicted bcMx1 protein contains 631 amino acids. bcMx1 contains a GTPase domain at the N-terminnus, a "central interactive domain" in the middle and a GTPase effector domain at the C-terminus. bcMx1 mRNA was constitutively transcribed in all tissues tested, including the heart, liver, spleen, kidney, intestine, muscle, skin and gill; and bcMx1 mRNA levels increased in all but the gill after grass carp reovirus (GCRV) or viraemia of carp virus (SVCV) infection. Quantitative PCR analysis of Mylopharyngodon piceus fin (MPF) cells indicated that bcMx1 mRNA levels increased after GCRV or SVCV infection at different multiplicities of infection (MOI). Western blotting demonstrated that the molecular weight of bcMx1 is similar to 75 kDa and immunofluorescent staining data of both HeLa cells and EPC cells showed that bcMx1 is a cytosolic protein. EPC cells transfected with plasmid expressing bcMx1 showed increased antiviral activity against SVCV and GCRV. All our data suggest that bcMx1 is an antiviral protein in the innate immune response of the black carp. (C) 2016 Elsevier Ltd. All rights reserved. |
Keyword | Black carp Mx Innate immunity |
DOI | 10.1016/j.fsi.2016.10.002 |
Funding Organization | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province |
Indexed By | SCI |
Language | 英语 |
Funding Project | National Natural Science Foundation of China[31272634] ; National Natural Science Foundation of China[81471963] ; National Natural Science Foundation of China[31502159] ; Fund of FEBL[2013FB02] ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province[20134486] |
WOS Research Area | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
WOS Subject | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
WOS ID | WOS:000387523100068 |
WOS Keyword | PANCREATIC NECROSIS VIRUS ; PATTERN-RECOGNITION RECEPTORS ; INTERFERON-STIMULATED GENES ; SEABREAM SPARUS-AURATA ; IKK-EPSILON GENE ; INFLUENZA-VIRUS ; MYLOPHARYNGODON-PICEUS ; PATHOGEN RECOGNITION ; VIRAL-INFECTIONS ; RESISTANCE |
Publisher | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
Funding Organization | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Fund of FEBL ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province ; Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.ihb.ac.cn/handle/342005/34105 |
Collection | 淡水生态学研究中心_期刊论文 |
Corresponding Author | Tang, Luosheng; Feng, Hao |
Affiliation | 1.Hunan Normal Univ, Coll Life Sci, Minist Educ China, Key Lab Prot Chem & Dev Biol, Changsha 410081, Hunan, Peoples R China 2.Cent S Univ, Xiangya Hosp 2, Changsha 410011, Hunan, Peoples R China 3.State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
Recommended Citation GB/T 7714 | Xiao, Jun,Yan, Jun,Chen, Hui,et al. Mx1 of black carp functions importantly in the antiviral innate immune response[J]. FISH & SHELLFISH IMMUNOLOGY,2016,58(1):584-592. |
APA | Xiao, Jun.,Yan, Jun.,Chen, Hui.,Li, Jun.,Tian, Yu.,...&Feng, Hao.(2016).Mx1 of black carp functions importantly in the antiviral innate immune response.FISH & SHELLFISH IMMUNOLOGY,58(1),584-592. |
MLA | Xiao, Jun,et al."Mx1 of black carp functions importantly in the antiviral innate immune response".FISH & SHELLFISH IMMUNOLOGY 58.1(2016):584-592. |
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