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MicroRNAs modulate adaption to multiple abiotic stresses in Chlamydomonas reinhardtii
Gao, Xiang1,2; Zhang, Fengge1,2; Hu, Jinlu1,2; Cai, Wenkai1,2; Shan, Ge3; Dai, Dongsheng4; Huang, Kaiyao1; Wang, Gaohong1
2016-12-02
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
Volume6Issue:1Pages:15
Abstract

MicroRNAs play an important role in abiotic stress responses in higher plants and animals, but their role in stress adaptation in algae remains unknown. In this study, the expression of identified and putative miRNAs in Chlamydomonas reinhardtii was assessed using quantitative polymerase chain reaction; some of the miRNAs (Cre-miR906-3p) were up-regulated, whereas others (Cre-miR910) were downregulated when the species was subjected to multiple abiotic stresses. With degradome sequencing data, we also identified ATP4 (the d-subunit of ATP synthase) and NCR2 (NADPH: cytochrome P450 reductase) as one of the several targets of Cre-miR906-3p and Cre-miR910, respectively. Q-PCR data indicated that ATP4, which was expressed inversely in relation to Cre-miR906-3p under stress conditions. Overexpressing of Cre-miR906-3p enhanced resistance to multiple stresses; conversely, overexpressing of ATP4 produced the opposite effect. These data of Q-PCR, degradome sequencing and adaptation of overexpressing lines indicated that Cre-miR906-3p and its target ATP4 were a part of the same pathway for stress adaptation. We found that Cre-miR910 and its target NCR2 were also a part of this pathway. Overexpressing of Cre-miR910 decreased, whereas that of NCR2 increased the adaption to multiple stresses. Our findings suggest that the two classes of miRNAs synergistically mediate stress adaptation in algae.

DOI10.1038/srep38228
Funding OrganizationNatural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight
Indexed BySCI
Language英语
Funding ProjectNatural Science Foundation of China[30970688] ; China Torch Program[2015GH581522] ; Project of Chinese Manned Spaceflight
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000389122400001
WOS KeywordMITOCHONDRIAL ATP SYNTHASE ; TRANSLATIONAL REPRESSION ; MESSENGER-RNA ; RESPONSIVE MICRORNAS ; ARTIFICIAL MICRORNAS ; REGULATED MICRORNAS ; ARABIDOPSIS ; IDENTIFICATION ; PLANTS ; GENES
PublisherNATURE PUBLISHING GROUP
Funding OrganizationNatural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; China Torch Program ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight ; Project of Chinese Manned Spaceflight
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/33984
Collection水环境工程研究中心_期刊论文
Corresponding AuthorWang, Gaohong
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Univ Sci & Technol, Sch Life Sci, Hefei 230022, Peoples R China
4.Wuxi Biortus Biosci Co Ltd, Jiangyin 214437, Jiangsu, Peoples R China
Recommended Citation
GB/T 7714
Gao, Xiang,Zhang, Fengge,Hu, Jinlu,et al. MicroRNAs modulate adaption to multiple abiotic stresses in Chlamydomonas reinhardtii[J]. SCIENTIFIC REPORTS,2016,6(1):15.
APA Gao, Xiang.,Zhang, Fengge.,Hu, Jinlu.,Cai, Wenkai.,Shan, Ge.,...&Wang, Gaohong.(2016).MicroRNAs modulate adaption to multiple abiotic stresses in Chlamydomonas reinhardtii.SCIENTIFIC REPORTS,6(1),15.
MLA Gao, Xiang,et al."MicroRNAs modulate adaption to multiple abiotic stresses in Chlamydomonas reinhardtii".SCIENTIFIC REPORTS 6.1(2016):15.
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