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题名: Heterologous expression of mlrA gene originated from Novosphingobium sp THN1 to degrade microcystin-RR and identify the first step involved in degradation pathway
作者: Wang, Ruiping1; Li, Jieming1; Jiang, Yongguang2; Lu, Zhijiang3; Li, Renhui4; Li, Ji1
关键词: Biodegradation ; Heterologous expression ; Microcystin-RR ; mlrA gene ; Novosphingobium sp THN1
刊名: CHEMOSPHERE
发表日期: 2017-10-01
DOI: 10.1016/j.chemosphere.2017.05.086
卷: 184, 页:159-167
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Environmental Sciences
研究领域[WOS]: Environmental Sciences & Ecology
英文摘要: Information on the catalytic role of mlrA gene-encoded enzyme (MlrA) in microcystin-RR (MC-RR) biodegradation was limited. This study succeeded in expressing mlrA homolog of Novosphingobium sp. THN1 in heterologous host for the first time, by constructing a recombinant bacterium. Mass spectrometric analysis showed that the recombinant MlrA hydrolyzed MC-RR into linear intermediate product by cleaving the peptide bond between Adda and arginine residue, greatly detoxifying MC-RR. This finding clearly manifested that the MlrA homolog of THN1 strain possesses its original catalytic function, and ring-opening constituted the first step in MC-RR biodegradation pathway of THN1 strain. Moreover, MC-RR degradation by intact recombinant cells and cell-free crude enzyme (CE) from recombinant was compared. Results exhibited that intact recombinant was able to degrade 20 mu g mL(-1) MC-RR more quickly than CE, with the maximum rate of 9.22 mu g mL(-1) h(-1) in the first 8 h. Thus, this study provided new insights on the catalytic activity and roles of MIrA originated from THN1 strain in MC-RR biodegradation process, which lay a foundation for efficiently removing and detoxifying MC-RR, and exploring downstream steps in MC-RR biodegradation pathway of THN1 strain. (C) 2017 Elsevier Ltd. All rights reserved.
关键词[WOS]: BIOLOGICAL TREATMENT FACILITY ; BACTERIAL-DEGRADATION ; NUTRIENT CONDITIONS ; ENZYMATIC PATHWAY ; PICHIA-PASTORIS ; BIODEGRADATION ; LR ; CLONING ; HEPATOTOXINS ; CYANOBACTERIA
语种: 英语
项目资助者: National Natural Science Foundation of China(31300434) ; Research Fund for the Doctoral Program of Higher Education of China(20130008120026)
WOS记录号: WOS:000407525500019
ISSN号: 0045-6535
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/33920
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.China Agr Univ, Coll Resources & Environm Sci, Beijing 100193, Peoples R China
2.Shenzhen Univ, Shenzhen Key Lab Marine Bioresource & Ecoenvironm, Shenzhen 518060, Guangdong, Peoples R China
3.China Inst Water Resources & Hydropower Res, Dept Irrigat & Drainage, Beijing 100048, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China
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