IHB OpenIR  > 藻类生物学及应用研究中心
Heterologous expression of mlrA gene originated from Novosphingobium sp THN1 to degrade microcystin-RR and identify the first step involved in degradation pathway
Wang, Ruiping1; Li, Jieming1; Jiang, Yongguang2; Lu, Zhijiang3; Li, Renhui4; Li, Ji1
2017-10-01
Source PublicationCHEMOSPHERE
ISSN0045-6535
Volume184Pages:159-167
AbstractInformation on the catalytic role of mlrA gene-encoded enzyme (MlrA) in microcystin-RR (MC-RR) biodegradation was limited. This study succeeded in expressing mlrA homolog of Novosphingobium sp. THN1 in heterologous host for the first time, by constructing a recombinant bacterium. Mass spectrometric analysis showed that the recombinant MlrA hydrolyzed MC-RR into linear intermediate product by cleaving the peptide bond between Adda and arginine residue, greatly detoxifying MC-RR. This finding clearly manifested that the MlrA homolog of THN1 strain possesses its original catalytic function, and ring-opening constituted the first step in MC-RR biodegradation pathway of THN1 strain. Moreover, MC-RR degradation by intact recombinant cells and cell-free crude enzyme (CE) from recombinant was compared. Results exhibited that intact recombinant was able to degrade 20 mu g mL(-1) MC-RR more quickly than CE, with the maximum rate of 9.22 mu g mL(-1) h(-1) in the first 8 h. Thus, this study provided new insights on the catalytic activity and roles of MIrA originated from THN1 strain in MC-RR biodegradation process, which lay a foundation for efficiently removing and detoxifying MC-RR, and exploring downstream steps in MC-RR biodegradation pathway of THN1 strain. (C) 2017 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordBiodegradation Heterologous Expression Microcystin-rr Mlra Gene NovoSphingobium Sp Thn1
DOI10.1016/j.chemosphere.2017.05.086
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Natural Science Foundation of China(31300434) ; National Natural Science Foundation of China(31300434) ; Research Fund for the Doctoral Program of Higher Education of China(20130008120026) ; Research Fund for the Doctoral Program of Higher Education of China(20130008120026) ; National Natural Science Foundation of China(31300434) ; National Natural Science Foundation of China(31300434) ; Research Fund for the Doctoral Program of Higher Education of China(20130008120026) ; Research Fund for the Doctoral Program of Higher Education of China(20130008120026)
Language英语
WOS Research AreaEnvironmental Sciences & Ecology
WOS SubjectEnvironmental Sciences
WOS KeywordBIOLOGICAL TREATMENT FACILITY ; BACTERIAL-DEGRADATION ; NUTRIENT CONDITIONS ; ENZYMATIC PATHWAY ; PICHIA-PASTORIS ; BIODEGRADATION ; LR ; CLONING ; HEPATOTOXINS ; CYANOBACTERIA
WOS IDWOS:000407525500019
Funding OrganizationNational Natural Science Foundation of China(31300434) ; National Natural Science Foundation of China(31300434) ; Research Fund for the Doctoral Program of Higher Education of China(20130008120026) ; Research Fund for the Doctoral Program of Higher Education of China(20130008120026) ; National Natural Science Foundation of China(31300434) ; National Natural Science Foundation of China(31300434) ; Research Fund for the Doctoral Program of Higher Education of China(20130008120026) ; Research Fund for the Doctoral Program of Higher Education of China(20130008120026)
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/33920
Collection藻类生物学及应用研究中心
Affiliation1.China Agr Univ, Coll Resources & Environm Sci, Beijing 100193, Peoples R China
2.Shenzhen Univ, Shenzhen Key Lab Marine Bioresource & Ecoenvironm, Shenzhen 518060, Guangdong, Peoples R China
3.China Inst Water Resources & Hydropower Res, Dept Irrigat & Drainage, Beijing 100048, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Wang, Ruiping,Li, Jieming,Jiang, Yongguang,et al. Heterologous expression of mlrA gene originated from Novosphingobium sp THN1 to degrade microcystin-RR and identify the first step involved in degradation pathway[J]. CHEMOSPHERE,2017,184:159-167.
APA Wang, Ruiping,Li, Jieming,Jiang, Yongguang,Lu, Zhijiang,Li, Renhui,&Li, Ji.(2017).Heterologous expression of mlrA gene originated from Novosphingobium sp THN1 to degrade microcystin-RR and identify the first step involved in degradation pathway.CHEMOSPHERE,184,159-167.
MLA Wang, Ruiping,et al."Heterologous expression of mlrA gene originated from Novosphingobium sp THN1 to degrade microcystin-RR and identify the first step involved in degradation pathway".CHEMOSPHERE 184(2017):159-167.
Files in This Item:
File Name/Size DocType Version Access License
1-s2.0-S004565351730(2137KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, Ruiping]'s Articles
[Li, Jieming]'s Articles
[Jiang, Yongguang]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Ruiping]'s Articles
[Li, Jieming]'s Articles
[Jiang, Yongguang]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Ruiping]'s Articles
[Li, Jieming]'s Articles
[Jiang, Yongguang]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 1-s2.0-S0045653517307907-main.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.