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题名: Cloning, expression pattern and promoter functional analysis of cyp19a1a gene in miiuy croaker
作者: Huang, Wei1, 2; Yang, Pan1; Lv, Zhenming1; Wu, Changwen1; Gui, Jianfang3; Lou, Bao2
关键词: cyp19a1a ; Vitellogenesis ; Promoter analysis ; Miichthys miiuy
刊名: GENE
发表日期: 2017-09-05
DOI: 10.1016/j.gene.2017.06.042
卷: 627, 页:271-277
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Genetics & Heredity
研究领域[WOS]: Genetics & Heredity
英文摘要: Gonadal-specific aromatase encoded by cyp19a1a is the important enzyme controlling estrogen biosynthesis in teleosts. In the present study, the cDNA sequence of cyp19a1a was cloned and characterized from miiuy croaker Miichthys miiuy. The cDNA encoded a protein of 519 amino acids with five structural regions. Higher identities of amino acid sequences and conserved structural regions were found between Mmcyp19a1a and other cyp19a1a genes. In addition, Mmcyp19a1a was clustered together with other seawater fishes. Immunohistochemical analysis revealed that Mmcyp19a1a was localized exclusively in the cytoplasmic of thecal and granulosa cells surrounding the oocytes. Both the protein and mRNA levels of Mmcyp19a1a were increased significantly at the stage III follicles (mid-vitellogenic) and then decreased along with vitellogenesis.
关键词[WOS]: MEDAKA ORYZIAS-LATIPES ; FLOUNDER PARALICHTHYS-OLIVACEUS ; TILAPIA OREOCHROMIS-NILOTICUS ; GONADAL SEX-DIFFERENTIATION ; TELEOST FISH ; DIMORPHIC EXPRESSION ; NILE TILAPIA ; AROMATASE IMMUNOREACTIVITY ; ACIPENSER-SINENSIS ; CHINESE STURGEON
语种: 英语
项目资助者: Zhejiang Provincial Natural Science Foundation of China(LY14C190003) ; Special Fund for Agro-scientific Research in the Public Interest(201203065-07) ; Research Project of the Department of Education of Zhejiang Province(Y201328305)
WOS记录号: WOS:000407658000036
ISSN号: 0378-1119
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/33715
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Zhejiang Ocean Univ, Coll Marine Sci & Technol, Natl Engn Res Ctr Marine Facil Aquaculture, Zhoushan 316022, Zhejiang, Peoples R China
2.Key Lab Marine Culture & Enhancement Zhejiang Pro, Zhoushan 316022, Zhejiang, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
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