IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心
Cloning, expression pattern and promoter functional analysis of cyp19a1a gene in miiuy croaker
Huang, Wei1,2; Yang, Pan1; Lv, Zhenming1; Wu, Changwen1; Gui, Jianfang3; Lou, Bao2
2017-09-05
Source PublicationGENE
ISSN0378-1119
Volume627Pages:271-277
AbstractGonadal-specific aromatase encoded by cyp19a1a is the important enzyme controlling estrogen biosynthesis in teleosts. In the present study, the cDNA sequence of cyp19a1a was cloned and characterized from miiuy croaker Miichthys miiuy. The cDNA encoded a protein of 519 amino acids with five structural regions. Higher identities of amino acid sequences and conserved structural regions were found between Mmcyp19a1a and other cyp19a1a genes. In addition, Mmcyp19a1a was clustered together with other seawater fishes. Immunohistochemical analysis revealed that Mmcyp19a1a was localized exclusively in the cytoplasmic of thecal and granulosa cells surrounding the oocytes. Both the protein and mRNA levels of Mmcyp19a1a were increased significantly at the stage III follicles (mid-vitellogenic) and then decreased along with vitellogenesis.
SubtypeArticle
KeywordCyp19a1a Vitellogenesis Promoter Analysis Miichthys Miiuy
DOI10.1016/j.gene.2017.06.042
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationZhejiang Provincial Natural Science Foundation of China(LY14C190003) ; Zhejiang Provincial Natural Science Foundation of China(LY14C190003) ; Special Fund for Agro-scientific Research in the Public Interest(201203065-07) ; Special Fund for Agro-scientific Research in the Public Interest(201203065-07) ; Research Project of the Department of Education of Zhejiang Province(Y201328305) ; Research Project of the Department of Education of Zhejiang Province(Y201328305) ; Zhejiang Provincial Natural Science Foundation of China(LY14C190003) ; Zhejiang Provincial Natural Science Foundation of China(LY14C190003) ; Special Fund for Agro-scientific Research in the Public Interest(201203065-07) ; Special Fund for Agro-scientific Research in the Public Interest(201203065-07) ; Research Project of the Department of Education of Zhejiang Province(Y201328305) ; Research Project of the Department of Education of Zhejiang Province(Y201328305)
Language英语
WOS Research AreaGenetics & Heredity
WOS SubjectGenetics & Heredity
WOS KeywordMEDAKA ORYZIAS-LATIPES ; FLOUNDER PARALICHTHYS-OLIVACEUS ; TILAPIA OREOCHROMIS-NILOTICUS ; GONADAL SEX-DIFFERENTIATION ; TELEOST FISH ; DIMORPHIC EXPRESSION ; NILE TILAPIA ; AROMATASE IMMUNOREACTIVITY ; ACIPENSER-SINENSIS ; CHINESE STURGEON
WOS IDWOS:000407658000036
Funding OrganizationZhejiang Provincial Natural Science Foundation of China(LY14C190003) ; Zhejiang Provincial Natural Science Foundation of China(LY14C190003) ; Special Fund for Agro-scientific Research in the Public Interest(201203065-07) ; Special Fund for Agro-scientific Research in the Public Interest(201203065-07) ; Research Project of the Department of Education of Zhejiang Province(Y201328305) ; Research Project of the Department of Education of Zhejiang Province(Y201328305) ; Zhejiang Provincial Natural Science Foundation of China(LY14C190003) ; Zhejiang Provincial Natural Science Foundation of China(LY14C190003) ; Special Fund for Agro-scientific Research in the Public Interest(201203065-07) ; Special Fund for Agro-scientific Research in the Public Interest(201203065-07) ; Research Project of the Department of Education of Zhejiang Province(Y201328305) ; Research Project of the Department of Education of Zhejiang Province(Y201328305)
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/33715
Collection鱼类生物学及渔业生物技术研究中心
Affiliation1.Zhejiang Ocean Univ, Coll Marine Sci & Technol, Natl Engn Res Ctr Marine Facil Aquaculture, Zhoushan 316022, Zhejiang, Peoples R China
2.Key Lab Marine Culture & Enhancement Zhejiang Pro, Zhoushan 316022, Zhejiang, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Huang, Wei,Yang, Pan,Lv, Zhenming,et al. Cloning, expression pattern and promoter functional analysis of cyp19a1a gene in miiuy croaker[J]. GENE,2017,627:271-277.
APA Huang, Wei,Yang, Pan,Lv, Zhenming,Wu, Changwen,Gui, Jianfang,&Lou, Bao.(2017).Cloning, expression pattern and promoter functional analysis of cyp19a1a gene in miiuy croaker.GENE,627,271-277.
MLA Huang, Wei,et al."Cloning, expression pattern and promoter functional analysis of cyp19a1a gene in miiuy croaker".GENE 627(2017):271-277.
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