IHB OpenIR
Full-length normalization subtractive hybridization analysis provides new insights into sexual precocity and ovarian development of red swamp crayfish Procambarus clarkii
Tan, Hong-Yue1; Shao, Guang-Ming1,2; Kang, Peng-Fei1; Wang, Yu-Feng1
2017-02-01
Source PublicationAQUACULTURE
ISSN0044-8486
Volume468Issue:1Pages:417-427
Abstract

The red swamp crayfish Procambarus clarkii is an important freshwater economic crayfish in Chinese inland due to its high market value and consumer demand. The sexual precocity in this species has received considerable attention in recent years because more and more individuals matured at small sizes, which devalues the commercial production. In this study, by the full-length normalization subtractive hybridization (FNSH) method we identified 16 differently expressed sequences between precocious and normal grown P. clarkii ovaries. Of the down-regulated sequences in precocious ovaries, one encodes a peptide which shared the highest identity to C1q-like family and contained a typical C1q domain at the C-terminal, thus it was named PcC1q-like. QRT-PCR analysis showed that PcC1q-like was transcribed specifically in ovary, with higher expression level in vitellogenic stages. In situ hybridization exhibited specific expression of PcC1q-like in follicular cells around the vitellogenic oocytes. Knockdown of PcC1q-like by injection of double strand RNA (dsRNA) resulted in disruption of follicular cells, decreased transcripts of vitellogenin (Vg) and vitellogenin receptor (VgR), and reduced progesterone content. These results suggest that PcC1q-like may promote ovarian development by stimulating the synthesis and transportation of vitellogenin into oocytes. These data provide insights into the molecular regulatory mechanisms of sexual precocity and ovarian development in crustaceans. Statement of relevance: The red swamp crayfish Procambarus clarkii is one of the most important freshwater aqua culture species in Chinese inland. We screened 16 sequences that are potentially involved in sexual precocity of P. clarkii. One candidate was demonstrated to be a novel ovary-specific C1q-like gene of P. clarkii, thus named PcC1q-like. Knockdown of PcC1q-like resulted in disruption of follicular cells, decreased transcripts of Vg and VgR, and reduced progesterone, suggesting its essential role in vitellogenic ovarian development of P. clarkii. (C) 2016 Elsevier B.V. All rights reserved.

KeywordProcambarus clarkii Sexual precocity C1q-like Ovarian development
DOI10.1016/j.aquaculture.2016.10.043
Funding OrganizationProgram for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city
Indexed BySCI
Language英语
Funding ProjectProgram for Academic Leader in Wuhan city[201051730554]
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000390735200054
WOS KeywordTUMOR-NECROSIS-FACTOR ; CRAB ERIOCHEIR-SINENSIS ; GIANT TIGER SHRIMP ; C1Q FAMILY-MEMBER ; MOLECULAR CHARACTERIZATION ; MACROBRACHIUM-NIPPONENSE ; PENAEUS-JAPONICUS ; KURUMA PRAWN ; VITELLOGENIN ; EXPRESSION
PublisherELSEVIER SCIENCE BV
Funding OrganizationProgram for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city ; Program for Academic Leader in Wuhan city
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/33434
Collection中科院水生所知识产出(2009年前)
Corresponding AuthorWang, Yu-Feng
Affiliation1.Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
Recommended Citation
GB/T 7714
Tan, Hong-Yue,Shao, Guang-Ming,Kang, Peng-Fei,et al. Full-length normalization subtractive hybridization analysis provides new insights into sexual precocity and ovarian development of red swamp crayfish Procambarus clarkii[J]. AQUACULTURE,2017,468(1):417-427.
APA Tan, Hong-Yue,Shao, Guang-Ming,Kang, Peng-Fei,&Wang, Yu-Feng.(2017).Full-length normalization subtractive hybridization analysis provides new insights into sexual precocity and ovarian development of red swamp crayfish Procambarus clarkii.AQUACULTURE,468(1),417-427.
MLA Tan, Hong-Yue,et al."Full-length normalization subtractive hybridization analysis provides new insights into sexual precocity and ovarian development of red swamp crayfish Procambarus clarkii".AQUACULTURE 468.1(2017):417-427.
Files in This Item: Download All
File Name/Size DocType Version Access License
1-s2.0-S004484861630(2270KB)期刊论文出版稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Tan, Hong-Yue]'s Articles
[Shao, Guang-Ming]'s Articles
[Kang, Peng-Fei]'s Articles
Baidu academic
Similar articles in Baidu academic
[Tan, Hong-Yue]'s Articles
[Shao, Guang-Ming]'s Articles
[Kang, Peng-Fei]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Tan, Hong-Yue]'s Articles
[Shao, Guang-Ming]'s Articles
[Kang, Peng-Fei]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 1-s2.0-S0044848616308468-main.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.