IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Beneficial effect and potential molecular mechanism of chloroquine on sperm motility and fertilizing ability in yellow catfish
Zhang, Jin1; Ma, Wenge1; Xie, Binyue1; Gui, Jian-Fang1,2; Mei, Jie1
2017-02-01
Source PublicationAQUACULTURE
ISSN0044-8486
Volume468Issue:1Pages:307-313
Abstract

Sperm motility is a critical determinant of male fertility in vertebrates including fish species. Chloroquine, an autophagy inhibitor, has been reported to own debated influence on sperm quality of mammalians. The objectives of this study were to determine the effect of chloroquine on sperm motility and fertilizing efficiency in yellow catfish, an important freshwater fish species in China. Gradient doses of chloroquine were intraperitoneally injected into yellow catfish that resulted in an increasing expression level of LC3-II and inhibition of autophagy. Subsequently, various sperm parameters were assessed with the computer-assisted sperm analysis (CASA) system. Chloroquine injection significantly increased straight line velocity (VSL), curvilinear velocity (VCL) and average path velocity (VAP) of sperm compared with the control. Consequently, 1 mu M chloroquine/g body weight was revealed to be an optimal dose for in vivo treatment, which led to elevation of fertility rate, whereas no effect on the hatching rate. The comparative transcriptome analysis between chloroquine-treated and control testes were conducted in order to figure out the possible molecular mechanism of chloroquine to regulate sperm motility. Interestingly, Gene Ontology (GO) analysis indicated that chloroquine treatment not only inhibited autophagy pathway but also significantly reduced toll-like receptor signaling pathway, suggesting a possible trade-off between male reproduction and immunity. KEGG analysis showed up-regulation of many pathways including PI3K-AKT signaling pathway which has been reported to be correlated with spermatogenesis and sperm maturation in yellow catfish. Our data reveals a potential trade-off between reproductive traits and immune function, and suggests that the application of chloroquine could improve sperm quality and fertilization efficiency in broodstock fish. Statement of relevance: Our research demonstrated that an optimal dose of chloroquine could efficiently inhibit autophagy and improve sperm motility and fertilization efficiency. The comparative transcriptome analysis between chloroquine-treated and control testes were conducted in order to figure out the possible molecular mechanism of chloroquine to regulate sperm mobility. (C) 2016 Elsevier B.V. All rights reserved.

KeywordChloroquine Sperm motility Fertilizing ability Autophagy Toll-like receptor
DOI10.1016/j.aquaculture.2016.10.028
Funding OrganizationFundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities
Indexed BySCI
Language英语
Funding ProjectFundamental Research Funds for the Central Universities[2662015PY101]
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000390735200041
WOS KeywordTRADE-OFFS ; SPERMATOZOA ; REPRODUCTION ; QUALITY ; IMMUNITY ; FISH ; AUTOPHAGY ; DEFENSE ; INJURY ; GENES
PublisherELSEVIER SCIENCE BV
Funding OrganizationFundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/33425
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorMei, Jie
Affiliation1.Huazhong Agr Univ, Freshwater Aquaculture Collaborat Innovat Ctr Hub, Minist Agr, Coll Fisheries,Key Lab Freshwater Anim Breeding, Wuhan 430070, Peoples R China
2.Chinese Acad Sci, Univ Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Jin,Ma, Wenge,Xie, Binyue,et al. Beneficial effect and potential molecular mechanism of chloroquine on sperm motility and fertilizing ability in yellow catfish[J]. AQUACULTURE,2017,468(1):307-313.
APA Zhang, Jin,Ma, Wenge,Xie, Binyue,Gui, Jian-Fang,&Mei, Jie.(2017).Beneficial effect and potential molecular mechanism of chloroquine on sperm motility and fertilizing ability in yellow catfish.AQUACULTURE,468(1),307-313.
MLA Zhang, Jin,et al."Beneficial effect and potential molecular mechanism of chloroquine on sperm motility and fertilizing ability in yellow catfish".AQUACULTURE 468.1(2017):307-313.
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