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Comparative Analysis of Ciliary Membranes and Ectosomes
Long, Huan1; Zhang, Fan1,2; Xu, Nannan1,2; Liu, Gai1; Diener, Dennis R.3; Rosenbaum, Joel L.3; Huang, Kaiyao1
2016-12-19
Source PublicationCURRENT BIOLOGY
ISSN0960-9822
Volume26Issue:24Pages:3327-3335
Abstract

Primary and motile cilia/flagella function as cellular antennae, receiving signals from the environment and subsequently activating signaling pathways that are critical for cellular homeostasis and differentiation [1-3]. Recent work with the green alga Chlamydomonas and the nematode C. elegans demonstrated that ectosomes can be released from the cilium and can mediate the intercellular communication [4-9]. To better understand the function of flagellar ectosomes, we have compared their protein composition to that of the flagellar membrane from which they are derived. Ectosomes released from flagella have a unique protein composition, being enriched in a subset of flagellar membrane proteins, proteases, proteins from the endosomal sorting complex required for transport (ESCRT) [10-12], small GTPases, and ubiquitinated proteins. Live imaging showed that an ESCRT-related protein (PDCD6) was enriched in ectosomes released from flagella during gamete activation. We devised a sensitive and rapid assay to monitor ectosome release using luciferase fused to PDCD6 and a mutated ubiquitin. Ectosome release increased when cells underwent flagellar resorption. Knockdown of two ESCRT-related proteins, PDCD6 and VPS4, attenuated ectosome release during flagellar shortening and shortening was slowed. These data suggest that the ESCRT proteins mediate ectosome release and thereby influence flagellar shortening in Chlamydomonas. In addition, the prevalence of receptors such as agglutinin and ubiquitinated proteins in ciliary ectosomes suggests that they are involved in cell signaling and turnover of ciliary proteins.

DOI10.1016/j.cub.2016.09.055
Funding OrganizationNational Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH
Indexed BySCI
Language英语
Funding ProjectNational Nature Science Foundation of China[31171287] ; National Nature Science Foundation of China[31401154] ; NIH[GM014642]
WOS Research AreaBiochemistry & Molecular Biology ; Cell Biology
WOS SubjectBiochemistry & Molecular Biology ; Cell Biology
WOS IDWOS:000390666200026
WOS KeywordEXTRACELLULAR VESICLES ; CHLAMYDOMONAS-REINHARDTII ; ESCRT PATHWAY ; FLAGELLA ; ALIX ; BIOGENESIS ; PROTEINS ; EXOSOMES ; REVEALS ; CILIUM
PublisherCELL PRESS
Funding OrganizationNational Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH
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Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/33409
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorHuang, Kaiyao
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Recommended Citation
GB/T 7714
Long, Huan,Zhang, Fan,Xu, Nannan,et al. Comparative Analysis of Ciliary Membranes and Ectosomes[J]. CURRENT BIOLOGY,2016,26(24):3327-3335.
APA Long, Huan.,Zhang, Fan.,Xu, Nannan.,Liu, Gai.,Diener, Dennis R..,...&Huang, Kaiyao.(2016).Comparative Analysis of Ciliary Membranes and Ectosomes.CURRENT BIOLOGY,26(24),3327-3335.
MLA Long, Huan,et al."Comparative Analysis of Ciliary Membranes and Ectosomes".CURRENT BIOLOGY 26.24(2016):3327-3335.
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