Dietary myo-inositol deficiency decreased the growth performances and impaired intestinal physical barrier function partly relating to nrf2, jnk, e2f4 and mlck signaling in young grass carp (Ctenopharyngodon idella)
Li, Shuang-An1; Jiang, Wei-Dan1,2,3; Feng, Lin1,2,3; Liu, Yang1,2,3; Wu, Pei1,2,3; Jiang, Jun1,2,3; Kuang, Sheng-Yao4; Tang, Ling4; Tang, Wu-Neng4; Zhang, Yong-An5; Tang, Xu6; Shi, He-Qun7; Zhou, Xiao-Qiu1,2,3
2017-08-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume67Pages:475-492
AbstractIn this study, we investigated the effects of dietary myo-inositol on the growth and intestinal physical barrier functions of young grass carp (Ctenopharyngodon idella). A total of 540 young grass carp (221.83 +/- 0.84 g) were fed six diets containing graded levels of myo-inositol (27.0, 137.9, 286.8, 438.6, 587.7 and 737.3 mg/kg) for 10 weeks. After the growth trial, fish were challenged with Aeromonas hydrophila for 14 days. The results indicated that compared with optimal myo-inositol levels, myo-inositol deficiency (27.0 mg/kg diet): (1) decreased glutathione (GSH) contents and antioxidant enzymes activities, and down-regulated the mRNA levels of antioxidant enzymes [not glutathione-S-transferase (gst) pl and gstp2] and NF-E2-related factor 2 (nrf2), whereas up-regulated the reactive oxygen species (ROS), malondialdehyde (MDA) and protein carbonyl (PC) contents, and the mRNA levels of Kelchlike-ECH-associated protein 1 (keap1) in three intestinal segments of young grass carp (P < 0.05). (2) Up-regulated cysteinyl aspartic acid-protease (caspase)-2, -3, -7, -8, -9, apoptotic protease activating factor-1 (apaf-1), Bcl2-associated X protein (bax), fas ligand (fasl), gen-activated protein kinase (p38mapk) and c-Jun N-terminal protein kinase (ink) mRNA levels, whereas down-regulated B-cell lymphoma-2 (bcl-2), inhibitor of apoptosis proteins (iap) and myeloid cell leukemia-1 (mcl-1) mRNA levels in three intestinal segments of young grass carp (P < 0.05). (3) Down-regulated mRNA levels of cell cycle proteins cyclin b, cyclin d, cyclin e and E2F transcription factor 4 (e2f4) in three intestinal segments of young grass carp (P < 0.05). (4) Down-regulated the mRNA levels of zonula occludens (zo) 1, zo-2, occludin, claudin-b, -c, -f, -3c, -7a, -7b as well as -11, and up-regulated the mRNA levels of claudin-12, -15a (not -15b) and myosin light chain kinase (mlck) in three intestinal segments of young grass carp (P < 0.05). All above data indicated that dietary myo-inositol deficiency could damage physical barrier function in three intestinal segments of fish. Finally, the myo-inositol requirements based on the percent weight gain (PWG), reactive oxygen species (ROS) contents in the proximal intestine (PI), relative mRNA levels of caspase-2 (PI), cyclin b (MI) as well as claudin-b (PI) were estimated to be 276.7, 304.1, 327.9, 416.7 and 313.2 mg/kg diet, respectively. (C) 2017 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordMyo-inositol Grass Carp (Ctenopharyngodon Idella) Intestine Antioxidant Apoptosis Proliferation Tight Junction
DOI10.1016/j.fsi.2017.06.032
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Natural Science Foundation of China(31001116 ; National Natural Science Foundation of China(31001116 ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Outstanding Talents and Innovative Team of Agricultural Scientific Research (Ministry of Agriculture) ; Outstanding Talents and Innovative Team of Agricultural Scientific Research (Ministry of Agriculture) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Key Fund Project of Sichuan Provincial Department of Education(14ZA0007) ; Key Fund Project of Sichuan Provincial Department of Education(14ZA0007) ; Science and Technology Support Programme of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Programme of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; 31272674 ; 31272674 ; 2013NC0045) ; 2013NC0045) ; 31302197) ; 31302197) ; National Natural Science Foundation of China(31001116 ; National Natural Science Foundation of China(31001116 ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Outstanding Talents and Innovative Team of Agricultural Scientific Research (Ministry of Agriculture) ; Outstanding Talents and Innovative Team of Agricultural Scientific Research (Ministry of Agriculture) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Key Fund Project of Sichuan Provincial Department of Education(14ZA0007) ; Key Fund Project of Sichuan Provincial Department of Education(14ZA0007) ; Science and Technology Support Programme of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Programme of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; 31272674 ; 31272674 ; 2013NC0045) ; 2013NC0045) ; 31302197) ; 31302197)
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS KeywordNF-KAPPA-B ; MESSENGER-RNA EXPRESSION ; TIGHT JUNCTION PROTEINS ; ANTIOXIDANT ENZYME-ACTIVITIES ; SALMON SALMO-SALAR ; AEROMONAS-HYDROPHILA ; IMMUNE-RESPONSE ; OXIDATIVE STRESS ; ATLANTIC SALMON ; ACID DEFICIENCY
WOS IDWOS:000406726300050
Funding OrganizationNational Natural Science Foundation of China(31001116 ; National Natural Science Foundation of China(31001116 ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Outstanding Talents and Innovative Team of Agricultural Scientific Research (Ministry of Agriculture) ; Outstanding Talents and Innovative Team of Agricultural Scientific Research (Ministry of Agriculture) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Key Fund Project of Sichuan Provincial Department of Education(14ZA0007) ; Key Fund Project of Sichuan Provincial Department of Education(14ZA0007) ; Science and Technology Support Programme of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Programme of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; 31272674 ; 31272674 ; 2013NC0045) ; 2013NC0045) ; 31302197) ; 31302197) ; National Natural Science Foundation of China(31001116 ; National Natural Science Foundation of China(31001116 ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Outstanding Talents and Innovative Team of Agricultural Scientific Research (Ministry of Agriculture) ; Outstanding Talents and Innovative Team of Agricultural Scientific Research (Ministry of Agriculture) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Key Fund Project of Sichuan Provincial Department of Education(14ZA0007) ; Key Fund Project of Sichuan Provincial Department of Education(14ZA0007) ; Science and Technology Support Programme of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Programme of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; 31272674 ; 31272674 ; 2013NC0045) ; 2013NC0045) ; 31302197) ; 31302197)
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/33392
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Affiliation1.Sichuan Agr Univ, Anim Nutr Inst, Chengdu 611130, Sichuan, Peoples R China
2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov, Chengdu 611130, Sichuan, Peoples R China
3.Sichuan Agr Univ, Minist Educ, Key Lab Anim Dis Resistance Nutr China, Chengdu 611130, Sichuan, Peoples R China
4.Sichuan Acad Anim Sci, Anim Nutr Inst, Chengdu 610066, Sichuan, Peoples R China
5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
6.Chengdu Mytech Biotech Co Ltd, Chengdu 610222, Sichuan, Peoples R China
7.Guangzhou Cohoo Biotech Res & Dev Ctr, Guangzhou 510663, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Li, Shuang-An,Jiang, Wei-Dan,Feng, Lin,et al. Dietary myo-inositol deficiency decreased the growth performances and impaired intestinal physical barrier function partly relating to nrf2, jnk, e2f4 and mlck signaling in young grass carp (Ctenopharyngodon idella)[J]. FISH & SHELLFISH IMMUNOLOGY,2017,67:475-492.
APA Li, Shuang-An.,Jiang, Wei-Dan.,Feng, Lin.,Liu, Yang.,Wu, Pei.,...&Zhou, Xiao-Qiu.(2017).Dietary myo-inositol deficiency decreased the growth performances and impaired intestinal physical barrier function partly relating to nrf2, jnk, e2f4 and mlck signaling in young grass carp (Ctenopharyngodon idella).FISH & SHELLFISH IMMUNOLOGY,67,475-492.
MLA Li, Shuang-An,et al."Dietary myo-inositol deficiency decreased the growth performances and impaired intestinal physical barrier function partly relating to nrf2, jnk, e2f4 and mlck signaling in young grass carp (Ctenopharyngodon idella)".FISH & SHELLFISH IMMUNOLOGY 67(2017):475-492.
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