IHB OpenIR  > 水环境工程研究中心  > 期刊论文
Denitrification characterization of dissolved oxygen microprofiles in lake surface sediment through analyzing abundance, expression, community composition and enzymatic activities of denitrifier functional genes
Hong,Pei1,2; Wu,Xingqiang1,2; Shu,Yilin3,4; Wang,ChunBo1,2; Tian,Cuicui1,2; Gong,Shihao1,2; Cai,Pei1,2; Donde,Oscar Omondi1,2; Xiao,Bangding1,2
Corresponding AuthorXiao,Bangding(bdxiao618@163.com)
2019-08-19
Source PublicationAMB Express
ISSN2191-0855
Volume9Issue:1
AbstractAbstractThe responses of denitrifiers and denitrification ability to dissolved oxygen (DO) concent in different layers of surface lake sediments are still poorly understood. Here, the optimal denitrification condition was constructed based on response surface methodology (RSM) to analyze the denitrification characteristics of surface sediments. The aerobic zone (AEZ), hypoxic zone (HYZ), up-anoxic zone (ANZ-1) and sub-anoxic zone (ANZ-2) were partitioned based on the oxygen contents, and sediments were collected using a customized-designed sub-millimeter scale sampling device. Integrated real-time quantitative PCR, Illumina Miseq-based sequencing and denitrifying enzyme activities analysis revealed that denitrification characteristics varied among different DO layers. Among the four layers, the DNA abundance and RNA expression levels of norB, nirS and nosZ were the highest at the aerobic layer, hypoxic layer and up-axoic layer, respectively. The hypoxia and up-anaerobic layer were the active nitrogen removal layers, since these two layers displayed the highest DNA abundance, RNA expression level and enzyme activities of denitrification functional genes. The abundance of major denitrifying bacteria showed significant differences among layers, with Azoarcus, Pseudogulbenkiania and Rhizobium identified as the main nirS, nirK and nosZ-based denitrifiers. Pearson’s correlation revealed that the response of denitrifiers to environmental factors differed greatly among DO layers. Furthermore, napA showed higher DNA abundance and RNA expression level in the aerobic and hypoxic layers than anaerobic layers, indicating that aerobic denitrifiers might play important roles at these layers.
KeywordResponse surface methodology (RSM) DO concentration Denitrifier Lake surface sediment Denitrification traits
DOI10.1186/s13568-019-0855-9
Language英语
WOS IDBMC:10.1186/s13568-019-0855-9
PublisherSpringer Berlin Heidelberg
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/33162
Collection水环境工程研究中心_期刊论文
Corresponding AuthorXiao,Bangding
Affiliation1.
2.
3.
4.
Recommended Citation
GB/T 7714
Hong,Pei,Wu,Xingqiang,Shu,Yilin,et al. Denitrification characterization of dissolved oxygen microprofiles in lake surface sediment through analyzing abundance, expression, community composition and enzymatic activities of denitrifier functional genes[J]. AMB Express,2019,9(1).
APA Hong,Pei.,Wu,Xingqiang.,Shu,Yilin.,Wang,ChunBo.,Tian,Cuicui.,...&Xiao,Bangding.(2019).Denitrification characterization of dissolved oxygen microprofiles in lake surface sediment through analyzing abundance, expression, community composition and enzymatic activities of denitrifier functional genes.AMB Express,9(1).
MLA Hong,Pei,et al."Denitrification characterization of dissolved oxygen microprofiles in lake surface sediment through analyzing abundance, expression, community composition and enzymatic activities of denitrifier functional genes".AMB Express 9.1(2019).
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Hong,Pei]'s Articles
[Wu,Xingqiang]'s Articles
[Shu,Yilin]'s Articles
Baidu academic
Similar articles in Baidu academic
[Hong,Pei]'s Articles
[Wu,Xingqiang]'s Articles
[Shu,Yilin]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Hong,Pei]'s Articles
[Wu,Xingqiang]'s Articles
[Shu,Yilin]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.