Identification of Triplophysa species from the Qinghai-Tibetan Plateau (QTP) and its adjacent regions through DNA barcodes

doi:10.1016/j.gene.2016.11.045

	Identification of Triplophysa species from the Qinghai-Tibetan Plateau (QTP) and its adjacent regions through DNA barcodes
	Li, Jiuxuan 1; Wang, Ying 1,2,4; Jin, Huifang 1; Li, Wujiao 1; Yan, Chaochao 1; Yan, Pengfei 1; Zhang, Xiuyue 1; He, Shunping 2; Song, Zhaobin 1,3
	2017-03-20
Source Publication	GENE
ISSN	0378-1119
Volume	605 Issue:1 Pages:12-19
Abstract	The genus Triplophysa is the largest and most difficult to identity morphologically fish group of superfamily Cobitoidea with 140 currently valid species, and is mainly distributed in the Qinghai-Tibetan Plateau (QTP) and adjacent regions. Most species within this genus possess highly similar morphological characteristics for adaption to the highland environment and are very difficult to be identified only based on morphology. The traditional species identification, mainly based on external morphological diagnostic characters, leads to inconsistent results in many cases. Herein, we provided a molecular method based on mitochondrial cytochrome c subunit I (COI) for the identification of Triplophysa fishes. Thirty-three Triplophysa species, 244 individuals, were used to determine whether barcoding was effective in discriminating species for this genus. The mean intraspecific and interspecific K2P distances ranged from 0 to 14.9% (mean, 2.9%) and 0 to 23.4% (mean, 9.7%), respectively. The tree-based analysis displayed most of species formed discrete clusters with strong bootstrap support values (>90%). The results showed that most of Triplophysa species could be identified by DNA barcode and indicated DNA barcode could be used as a molecular marker for these species. (C) 2016 Elsevier B.V. All rights reserved.
Keyword	Triplophysa DNA barcoding Intraspecific and interspecific genetic distance Phylogenetic tree Species identification
DOI	10.1016/j.gene.2016.11.045
Funding Organization	Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University
Indexed By	SCI
Language	英语
Funding Project	Program for New Century Excellent Talents in University[NCET-11-0347]
WOS Research Area	Genetics & Heredity
WOS Subject	Genetics & Heredity
WOS ID	WOS:000393261000003
WOS Keyword	FRESH-WATER FISHES ; PHYLOGENETIC TREES ; BALITORIDAE ; CHINA ; NEMACHEILINAE ; INFERENCE ; ACCURACY ; GUANGXI ; MRBAYES ; LOACH
Publisher	ELSEVIER SCIENCE BV
Funding Organization	Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University ; Program for New Century Excellent Talents in University
Citation statistics
Document Type	期刊论文
Identifier	http://ir.ihb.ac.cn/handle/342005/33070
Collection	水生生物多样性与资源保护研究中心_期刊论文
Corresponding Author	Song, Zhaobin
Affiliation	1.Sichuan Univ, Coll Life Sci, Sichuan Key Lab Conservat Biol Endangered Wildlif, Chengdu 610065, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China 3.Sichuan Univ, Coll Life Sci, Key Lab Bioresources & Ecoenvironm, Minist Educ, Chengdu 610065, Peoples R China 4.Jianghan Univ, Sch Life Sci, Wuhan 430056, Peoples R China
Recommended Citation GB/T 7714	Li, Jiuxuan,Wang, Ying,Jin, Huifang,et al. Identification of Triplophysa species from the Qinghai-Tibetan Plateau (QTP) and its adjacent regions through DNA barcodes[J]. GENE,2017,605(1):12-19.
APA	Li, Jiuxuan.,Wang, Ying.,Jin, Huifang.,Li, Wujiao.,Yan, Chaochao.,...&Song, Zhaobin.(2017).Identification of Triplophysa species from the Qinghai-Tibetan Plateau (QTP) and its adjacent regions through DNA barcodes.GENE,605(1),12-19.
MLA	Li, Jiuxuan,et al."Identification of Triplophysa species from the Qinghai-Tibetan Plateau (QTP) and its adjacent regions through DNA barcodes".GENE 605.1(2017):12-19.

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