IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心
A High-Density Genetic Linkage Map and QTL Fine Mapping for Body Weight in Crucian Carp (Carassius auratus) Using 2b-RAD Sequencing
Liu, Haiyang1,2; Fu, Beide1; Pang, Meixia1,2; Feng, Xiu1; Yu, Xiaomu1; Tong, Jingou1
2017-08-01
Source PublicationG3-GENES GENOMES GENETICS
ISSN2160-1836
Volume7Issue:8Pages:2473-2487
AbstractA high-resolution genetic linkage map is essential for a wide range of genetics and genomics studies such as comparative genomics analysis and QTL fine mapping. Crucian carp (Carassius auratus) is widely distributed in Eurasia, and is an important aquaculture fish worldwide. In this study, a high-density genetic linkage map was constructed for crucian carp using 2b-RAD technology. The consensusmap contains 8487 SNP markers, assigning to 50 linkage groups (LGs) and spanning 3762.88 cM, with an average marker interval of 0.44 cM and genome coverage of 98.8%. The female map had 4410 SNPs, and spanned 3500.42 cM (0.79 cM/marker), while the male map had 4625 SNPs and spanned 3346.33 cM (0.72 cM/marker). The average recombination ratio of female to male was 2.13: 1, and significant male-biased recombination suppressions were observed in LG47 and LG49. Comparative genomics analysis revealed a clear 2: 1 syntenic relationship between crucian carp LGs and chromosomes of zebrafish and grass carp, and a 1: 1 correspondence, but extensive chromosomal rearrangement, between crucian carp and common carp, providing evidence that crucian carp has experienced a fourth round of whole genome duplication (4R-WGD). Eight chromosome-wide QTL for body weight at 2 months after hatch were detected on five LGs, explaining 10.1-13.2% of the phenotypic variations. Potential candidate growth-related genes, such as an EGF-like domain and TGF-beta, were identified within the QTL intervals. This high-density genetic map and QTL analysis supplies a basis for genome evolutionary studies in cyprinid fishes, genome assembly, and QTL fine mapping for complex traits in crucian carp.
SubtypeArticle
KeywordCrucian Carp Snp Genetic Linkage Map Qtl Comparative Genomics Candidate Growth Gene
DOI10.1534/g3.117.041376
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationChinese Academy of Sciences(XDA08010405) ; Chinese Academy of Sciences(XDA08010405) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ05) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ05) ; Chinese Academy of Sciences(XDA08010405) ; Chinese Academy of Sciences(XDA08010405) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ05) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ05)
Language英语
WOS Research AreaGenetics & Heredity
WOS SubjectGenetics & Heredity
WOS KeywordWHOLE-GENOME DUPLICATION ; ZEBRAFISH DANIO-RERIO ; SALMON ONCORHYNCHUS-TSHAWYTSCHA ; GROWTH-RELATED TRAITS ; X COMMON CARP ; CYPRINUS-CARPIO ; PROVIDES INSIGHTS ; MICROSATELLITE MARKERS ; RECOMBINATION RATES ; GIBELIO BLOCH
WOS IDWOS:000407314000006
Funding OrganizationChinese Academy of Sciences(XDA08010405) ; Chinese Academy of Sciences(XDA08010405) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ05) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ05) ; Chinese Academy of Sciences(XDA08010405) ; Chinese Academy of Sciences(XDA08010405) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ05) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ05)
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/32986
Collection鱼类生物学及渔业生物技术研究中心
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7 Donghu South Rd, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Liu, Haiyang,Fu, Beide,Pang, Meixia,et al. A High-Density Genetic Linkage Map and QTL Fine Mapping for Body Weight in Crucian Carp (Carassius auratus) Using 2b-RAD Sequencing[J]. G3-GENES GENOMES GENETICS,2017,7(8):2473-2487.
APA Liu, Haiyang,Fu, Beide,Pang, Meixia,Feng, Xiu,Yu, Xiaomu,&Tong, Jingou.(2017).A High-Density Genetic Linkage Map and QTL Fine Mapping for Body Weight in Crucian Carp (Carassius auratus) Using 2b-RAD Sequencing.G3-GENES GENOMES GENETICS,7(8),2473-2487.
MLA Liu, Haiyang,et al."A High-Density Genetic Linkage Map and QTL Fine Mapping for Body Weight in Crucian Carp (Carassius auratus) Using 2b-RAD Sequencing".G3-GENES GENOMES GENETICS 7.8(2017):2473-2487.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Liu, Haiyang]'s Articles
[Fu, Beide]'s Articles
[Pang, Meixia]'s Articles
Baidu academic
Similar articles in Baidu academic
[Liu, Haiyang]'s Articles
[Fu, Beide]'s Articles
[Pang, Meixia]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Liu, Haiyang]'s Articles
[Fu, Beide]'s Articles
[Pang, Meixia]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.