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Polyphenolic allelochemical pyrogallic acid induces caspase-3(like)-dependent programmed cell death in the cyanobacterium Microcystis aeruginosa
Lu, Zhiying1,2; Sha, Jun1,2; Tian, Yun3; Zhang, Xuezhi1,2; Liu, Biyun3; Wu, Zhenbin3
2017
Source PublicationALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
ISSN2211-9264
Volume21Issue:1Pages:148-155
Abstract

The naturally occurring polyphenolic allelochemical pyrogallic acid (PA) is secreted by the aquatic vascular plant Myriophyllum spicatum and exhibits strong inhibitory effects on harmful cyanobacteria; however, the mechanisms underlying this inhibitory effect are largely unknown. In the current study, the biochemical and morphological changes that occurred during the PA-induced cell death of Microcystis aeruginosa and the mechanisms underlying these changeswere investigated. PA exposure inhibited the growth ofM. aeruginosa and significantly elevated caspase-3(-like) activities. The caspase-3 inhibitor aldehyde-terminated tetrapeptide (Ac-DEVD-CHO) abrogated the PA-induced caspase-3(-like) activities, which resulted in the alleviation of PA-induced growth inhibition. WhenM. aeruginosa cellswere exposed to 14 mgL(-1) PA for 72 h, nucleoid disintegration, photosynthetic lamellae rupture, pyknosis formation, vacuolation and DNA fragmentation were observed. The alignment of caspase domains showed that the key functional regions were conserved and showed remarkable orthology among different M. aeruginosa strains. Furthermore, a gas chromatography-mass spectrum analysis indicated that PA was gradually degraded. In addition, elevated intracellular reactive oxygen species (ROS) were detected. These results suggested that the production of ROS during the autoxidation of PA might act as a mediator that led to caspase-3(-like) activation and the subsequent initiation of programmed cell death in the cyanobacterium M. aeruginosa. (C) 2016 Elsevier B. V. All rights reserved.

KeywordAllelopathic interaction Caspase Sell death DNA fragmentation Pyrogallic acid Reactive oxygen species
DOI10.1016/j.algal.2016.11.007
Funding OrganizationNational Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China
Indexed BySCI
Language英语
Funding ProjectNational Nature Science Foundation of China[51178452] ; National Nature Science Foundation of China[50909091] ; National Nature Science Foundation of China[31123001]
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000396365500017
WOS KeywordOXIDATIVE STRESS ; MYRIOPHYLLUM-SPICATUM ; UNICELLULAR CHLOROPHYTE ; SUBMERGED MACROPHYTES ; ANNEXIN-V ; APOPTOSIS ; MECHANISM ; SYSTEM ; LAKE ; PHYTOPLANKTON
PublisherELSEVIER SCIENCE BV
Funding OrganizationNational Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China
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Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/32977
Collection水环境工程研究中心_期刊论文
Corresponding AuthorZhang, Xuezhi; Wu, Zhenbin
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Ctr Microalgal Biotechnol & Biofuels, Wuhan, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Lu, Zhiying,Sha, Jun,Tian, Yun,et al. Polyphenolic allelochemical pyrogallic acid induces caspase-3(like)-dependent programmed cell death in the cyanobacterium Microcystis aeruginosa[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2017,21(1):148-155.
APA Lu, Zhiying,Sha, Jun,Tian, Yun,Zhang, Xuezhi,Liu, Biyun,&Wu, Zhenbin.(2017).Polyphenolic allelochemical pyrogallic acid induces caspase-3(like)-dependent programmed cell death in the cyanobacterium Microcystis aeruginosa.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,21(1),148-155.
MLA Lu, Zhiying,et al."Polyphenolic allelochemical pyrogallic acid induces caspase-3(like)-dependent programmed cell death in the cyanobacterium Microcystis aeruginosa".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 21.1(2017):148-155.
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