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题名: 3-Hydroxyisobutyryl-CoA hydrolase involved in isoleucine catabolism regulates triacylglycerol accumulation in Phaeodactylum tricornutum
作者: Pan, Yufang1; Yang, Juan1, 2; Gong, Yangmin3; Li, Xiaolong1; Hu, Hanhua1
关键词: diatom ; propionyl-CoA carboxylase ; branched-chain amino acid catabolism ; beta-oxidation
刊名: PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
发表日期: 2017-09-05
DOI: 10.1098/rstb.2016.0409
卷: 372, 期:1728
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biology
研究领域[WOS]: Life Sciences & Biomedicine - Other Topics
英文摘要: Since methylmalonyl-CoA epimerase appears to be absent in the majority of photosynthetic organisms, including diatoms, (S)-methylmalonyl-CoA, the intermediate of isoleucine (Ile) catabolism, cannot be metabolized to (R)methylmalonyl-CoA then to succinyl-CoA. In this study, propionyl-CoA carboxylase (PCC) RNAi silenced strains and 3-hydroxyisobutyryl-CoA hydrolase (HIBCH) overexpression strains were constructed to elucidate the Ile degradation pathway and its influence on lipid accumulation in Phaeodactylum tricornutum based on growth, neutral lipid content and metabolite profile analysis. Knockdown of PCC disturbed the metabolism of Ile through propionyl-CoA to methylmalonyl-CoA, as illustrated by much higher Ile content at day 6. However, Ile decreased to comparable levels to the wild-type at day 10. PCC silencing redirected propionyl-CoA to acetyl-CoA via a modified beta-oxidation pathway, and transcript levels for some branched-chain amino acid (BCAA) degradation-related genes, especially HIBCH, significantly upregulated in the PCC mutant, which enhanced the BCAA degradations and thus resulted in higher triacylglycerol (TAG) content. Overexpression of HIBCH accelerates Ile degradation and results in a lowered Ile content in the overexpression strains, thus enhancing carbon skeletons to the tricarboxylic acid cycle and giving rise to increasing TAG accumulation. Our study provides a good strategy to obtain high-lipid-yield transgenic diatoms by modifying the propionyl-CoA metabolism.
关键词[WOS]: ARABIDOPSIS-THALIANA ; COMPARATIVE GENOMICS ; BETA-OXIDATION ; UREA CYCLE ; DIATOM ; METABOLISM ; EVOLUTION ; COA ; CARBOXYLASE ; COENZYME
语种: 英语
项目资助者: National Natural Science Foundation of China(41576144) ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2015FB12)
WOS记录号: WOS:000405676200014
ISSN号: 0962-8436
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/32976
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Agr Sci, Oil Crops Res Inst, Minist Agr, Key Lab Biol & Genet Improvement Oil Crops, Wuhan 430062, Peoples R China
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