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Targeted deep sequencing reveals high diversity and variable dominance of bloom-forming cyanobacteria in eutrophic lakes
Jiang, Yongguang1; Xiao, Peng1; Liu, Yang2; Wang, Jiangxin1; Li, Renhui3
2017-04-01
Source PublicationHARMFUL ALGAE
ISSN1568-9883
Volume64Pages:42-50
AbstractCyanobacterial blooms in eutrophic lakes are severe environmental problems worldwide. To characterize the spatiotemporal heterogeneity of cyanobacterial blooms, a high-throughput method is necessary for the specific detection of cyanobacteria. In this study, the cyanobacterial composition of three eutrophic waters in China (Taihu Lake, Dongqian Lake, and Dongzhen Reservoir) was determined by pyrosequencing the cpcBA intergenic spacer (cpcBA-IGS) of cyanobacteria. A total of 2585 OTUs were obtained from the normalized cpcBA-IGS sequence dataset at a distance of 0.05. The 238 most abundant OTUs contained 92% of the total sequences and were classified into six cyanobacterial groups. The water samples of Taihu Lake were dominated by Microcystis, mixed Nostocales species, Synechococcus, and unclassified cyanobacteria. Besides, all the samples from Taihu Lake were clustered together in the dendrograrn based on shared abundant OTUs. The cyanobacterial diversity in Dongqian Lake was dramatically decreased after sediment dredging and Synechococcus became exclusively dominant in this lake. The genus Synechococcus was also dominant in the surface water of Dongzhen Reservoir, while phylogenetically diverse cyanobacteria coexisted at a depth of 10 m in this reservoir. In summary, targeted deep sequencing based on cpcBA-IGS revealed a large diversity of bloom-forming cyanobacteria in eutrophic lakes and spatiotemporal changes in the composition of cyanobacterial communities. The genus Microcystis was the most abundant bloom-forming cyanobacteria in eutrophic lakes, while Synechococcus could be exclusively dominant under appropriate environmental conditions. (C) 2017 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordCyanobacterial Bloom Diversity Cpcba-igs Pyrosequencing Eutrophication
DOI10.1016/j.hal.2017.03.006
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Natural Science Foundation of China(31500071 ; National Natural Science Foundation of China(31500071 ; China Postdoctoral Science Foundation(2016T90801) ; China Postdoctoral Science Foundation(2016T90801) ; 31470310) ; 31470310) ; National Natural Science Foundation of China(31500071 ; National Natural Science Foundation of China(31500071 ; China Postdoctoral Science Foundation(2016T90801) ; China Postdoctoral Science Foundation(2016T90801) ; 31470310) ; 31470310)
Language英语
WOS Research AreaMarine & Freshwater Biology
WOS SubjectMarine & Freshwater Biology
WOS KeywordRIBOSOMAL-RNA GENES ; FRESH-WATER LAKE ; CLIMATE-CHANGE ; CYLINDROSPERMOPSIS-RACIBORSKII ; HIGH-THROUGHPUT ; TAIHU LAKE ; 16S RDNA ; CHINA ; SYNECHOCOCCUS ; MICROCYSTIS
WOS IDWOS:000400529900005
Funding OrganizationNational Natural Science Foundation of China(31500071 ; National Natural Science Foundation of China(31500071 ; China Postdoctoral Science Foundation(2016T90801) ; China Postdoctoral Science Foundation(2016T90801) ; 31470310) ; 31470310) ; National Natural Science Foundation of China(31500071 ; National Natural Science Foundation of China(31500071 ; China Postdoctoral Science Foundation(2016T90801) ; China Postdoctoral Science Foundation(2016T90801) ; 31470310) ; 31470310)
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/32975
Collection藻类生物学及应用研究中心
Affiliation1.Shenzhen Univ, Coll Life Sci & Oceanog, Shenzhen Key Lab Marine Bioresource & Ecoenvironm, Shenzhen 518060, Peoples R China
2.Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Jiang, Yongguang,Xiao, Peng,Liu, Yang,et al. Targeted deep sequencing reveals high diversity and variable dominance of bloom-forming cyanobacteria in eutrophic lakes[J]. HARMFUL ALGAE,2017,64:42-50.
APA Jiang, Yongguang,Xiao, Peng,Liu, Yang,Wang, Jiangxin,&Li, Renhui.(2017).Targeted deep sequencing reveals high diversity and variable dominance of bloom-forming cyanobacteria in eutrophic lakes.HARMFUL ALGAE,64,42-50.
MLA Jiang, Yongguang,et al."Targeted deep sequencing reveals high diversity and variable dominance of bloom-forming cyanobacteria in eutrophic lakes".HARMFUL ALGAE 64(2017):42-50.
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