IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
E2fl1 is a meiosis-specific transcription factor in the protist Tetrahymena thermophila
Zhang, Jing1,2; Tian, Miao1,3,4; Yan, Guan-xiong1,2; Shodhan, Anura3,4; Miao, Wei1
2017
Source PublicationCELL CYCLE
ISSN1538-4101
Volume16Issue:1Pages:123-135
Abstract

Members of the E2F family of transcription factors have been reported to regulate the expression of genes involved in cell cycle control, DNA replication, and DNA repair in multicellular eukaryotes. Here, E2FL1, a meiosis-specific E2F transcription factor gene, was identified in the model ciliate Tetrahymena thermophila. Loss of this gene resulted in meiotic arrest prior to anaphase I. The cytological experiments revealed that the meiotic homologous pairing was not affected in the absence of E2FL1, but the paired homologous chromosomes did not separate and assumed a peculiar tandem arrangement. This is the first time that an E2F family member has been shown to regulate meiotic events. Moreover, BrdU incorporation showed that DSB processing during meiosis was abnormal upon the deletion of E2FL1. Transcriptome sequencing analysis revealed that E2FL1 knockout decreased the expression of genes involved in DNA replication and DNA repair in T. thermophila, suggesting that the function of E2F is highly conserved in eukaryotes. In addition, E2FL1 deletion inhibited the expression of related homologous chromosome segregation genes in T. thermophila. The result may explain the meiotic arrest phenotype at anaphase I. Finally, by searching for E2F DNA-binding motifs in the entire T. thermophila genome, we identified 714 genes containing at least one E2F DNA-binding motif; of these, 235 downregulated represent putative E2FL1 target genes.

Keywordchromosome segregation DNA repair meiosis Tetrahymena thermophila transcription factor
DOI10.1080/15384101.2016.1259779
Funding OrganizationProjects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; 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Indexed BySCI
Language英语
Funding ProjectProjects of International Cooperation and Exchanges Ministry of Science and Technology of China[2013DFG32390] ; Natural Science Foundation of China[31525021] ; OEAD[CN10/2013] ; Austrian Science Fund[P27313-B20] ; Austrian Science Fund[W1238-B20]
WOS Research AreaCell Biology
WOS SubjectCell Biology
WOS IDWOS:000392737300022
WOS KeywordCELL-CYCLE CONTROL ; GENOME-WIDE IDENTIFICATION ; DNA-DAMAGE ; GENE-EXPRESSION ; NUCLEAR-LOCALIZATION ; GAMMA-TUBULIN ; TARGET GENES ; RNA-SEQ ; PROGRESSION ; FAMILY
PublisherTAYLOR & FRANCIS INC
Funding OrganizationProjects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; OEAD ; OEAD ; 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Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; OEAD ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund ; Austrian Science Fund
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/32937
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorMiao, Wei
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Univ Vienna, Dept Chromosome Biol, Ctr Mol Biol, Vienna, Austria
4.Univ Vienna, Max F Perutz Labs, Ctr Mol Biol, Vienna, Austria
Recommended Citation
GB/T 7714
Zhang, Jing,Tian, Miao,Yan, Guan-xiong,et al. E2fl1 is a meiosis-specific transcription factor in the protist Tetrahymena thermophila[J]. CELL CYCLE,2017,16(1):123-135.
APA Zhang, Jing,Tian, Miao,Yan, Guan-xiong,Shodhan, Anura,&Miao, Wei.(2017).E2fl1 is a meiosis-specific transcription factor in the protist Tetrahymena thermophila.CELL CYCLE,16(1),123-135.
MLA Zhang, Jing,et al."E2fl1 is a meiosis-specific transcription factor in the protist Tetrahymena thermophila".CELL CYCLE 16.1(2017):123-135.
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