E2fl1 is a meiosis-specific transcription factor in the protist Tetrahymena thermophila | |
Zhang, Jing1,2; Tian, Miao1,3,4; Yan, Guan-xiong1,2; Shodhan, Anura3,4; Miao, Wei1 | |
2017 | |
Source Publication | CELL CYCLE
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ISSN | 1538-4101 |
Volume | 16Issue:1Pages:123-135 |
Abstract | Members of the E2F family of transcription factors have been reported to regulate the expression of genes involved in cell cycle control, DNA replication, and DNA repair in multicellular eukaryotes. Here, E2FL1, a meiosis-specific E2F transcription factor gene, was identified in the model ciliate Tetrahymena thermophila. Loss of this gene resulted in meiotic arrest prior to anaphase I. The cytological experiments revealed that the meiotic homologous pairing was not affected in the absence of E2FL1, but the paired homologous chromosomes did not separate and assumed a peculiar tandem arrangement. This is the first time that an E2F family member has been shown to regulate meiotic events. Moreover, BrdU incorporation showed that DSB processing during meiosis was abnormal upon the deletion of E2FL1. Transcriptome sequencing analysis revealed that E2FL1 knockout decreased the expression of genes involved in DNA replication and DNA repair in T. thermophila, suggesting that the function of E2F is highly conserved in eukaryotes. In addition, E2FL1 deletion inhibited the expression of related homologous chromosome segregation genes in T. thermophila. The result may explain the meiotic arrest phenotype at anaphase I. Finally, by searching for E2F DNA-binding motifs in the entire T. thermophila genome, we identified 714 genes containing at least one E2F DNA-binding motif; of these, 235 downregulated represent putative E2FL1 target genes. |
Keyword | chromosome segregation DNA repair meiosis Tetrahymena thermophila transcription factor |
DOI | 10.1080/15384101.2016.1259779 |
Funding Organization | Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; 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Indexed By | SCI |
Language | 英语 |
Funding Project | Projects of International Cooperation and Exchanges Ministry of Science and Technology of China[2013DFG32390] ; Natural Science Foundation of China[31525021] ; OEAD[CN10/2013] ; Austrian Science Fund[P27313-B20] ; Austrian Science Fund[W1238-B20] |
WOS Research Area | Cell Biology |
WOS Subject | Cell Biology |
WOS ID | WOS:000392737300022 |
WOS Keyword | CELL-CYCLE CONTROL ; GENOME-WIDE IDENTIFICATION ; DNA-DAMAGE ; GENE-EXPRESSION ; NUCLEAR-LOCALIZATION ; GAMMA-TUBULIN ; TARGET GENES ; RNA-SEQ ; PROGRESSION ; FAMILY |
Publisher | TAYLOR & FRANCIS INC |
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Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.ihb.ac.cn/handle/342005/32937 |
Collection | 水生生物分子与细胞生物学研究中心_期刊论文 |
Corresponding Author | Miao, Wei |
Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Univ Vienna, Dept Chromosome Biol, Ctr Mol Biol, Vienna, Austria 4.Univ Vienna, Max F Perutz Labs, Ctr Mol Biol, Vienna, Austria |
Recommended Citation GB/T 7714 | Zhang, Jing,Tian, Miao,Yan, Guan-xiong,et al. E2fl1 is a meiosis-specific transcription factor in the protist Tetrahymena thermophila[J]. CELL CYCLE,2017,16(1):123-135. |
APA | Zhang, Jing,Tian, Miao,Yan, Guan-xiong,Shodhan, Anura,&Miao, Wei.(2017).E2fl1 is a meiosis-specific transcription factor in the protist Tetrahymena thermophila.CELL CYCLE,16(1),123-135. |
MLA | Zhang, Jing,et al."E2fl1 is a meiosis-specific transcription factor in the protist Tetrahymena thermophila".CELL CYCLE 16.1(2017):123-135. |
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