IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Molecular characterization and expression analysis of three subclasses of IgT in rainbow trout (Oncorhynchus mykiss)
Zhang, Nu1,2; Zhang, Xu-Jie1,3; Chen, Dan-Dan1; Sunyer, J. Oriol4; Zhang, Yong-An1
2017-05-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume70Issue:1Pages:94-105
Abstract

As the teleost specific immunoglobulin, IgT plays important roles in systemic and mucosal immunity. In the current study, in rainbow trout, we have cloned the heavy chain (Ig tau) genes of a secretory form of IgT2 as well as the membrane and secretory forms of a third IgT subclass, termed IgT3. Conserved cysteine and tryptophan residues that are crucial for the folding of the immunoglobulin domain as well as hydrophobic and hydrophilic residues within CART motif were identified in all IgT subclasses. Through analysis of the rainbow trout genome assembly, Ig tau 3 gene was found localized upstream of Ig tau l gene, while Ig tau 2 gene situated on another scaffold. At the transcriptional level, Ig tau 1 was mainly expressed in both systemic and mucosal lymphoid tissues, while Ig tau 2 was largely expressed in systemic lymphoid organs. After LPS and poly (I:C) treatment, Ig tau 1 and Ig tau 2 genes exhibited different expression profiles. Interestingly the transcriptional level of Ig tau 3 was negligible, although its protein product could be identified in trout serum. Importantly, a previously reported monoclonal antibody directed against trout IgT1 was able to recognize IgT2 and IgT3. These data demonstrate that there exist three subclasses of IgT in rainbow trout, and that their heavy chain genes display different expression patterns during stimulation. Overall, our data reflect the diversity and complexity of immunoglobulin in trout, thus provide a better understanding of the IgT system in the immune response of teleost fish. (C) 2017 Elsevier Ltd. All rights reserved.

KeywordIgT Subclass Immunoglobulin Rainbow trout
DOI10.1016/j.dci.2017.01.001
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; U.S. Department of Agriculture ; U.S. Department of Agriculture ; U.S. Department of Agriculture ; U.S. Department of Agriculture ; U.S. Department of Agriculture ; U.S. Department of Agriculture ; U.S. Department of Agriculture ; U.S. Department of Agriculture ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; U.S. Department of Agriculture ; U.S. Department of Agriculture ; U.S. Department of Agriculture ; U.S. Department of Agriculture ; U.S. Department of Agriculture ; U.S. Department of Agriculture ; U.S. Department of Agriculture ; U.S. Department of Agriculture ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; U.S. Department of Agriculture ; U.S. Department of Agriculture ; U.S. Department of Agriculture ; U.S. Department of Agriculture ; U.S. Department of Agriculture ; U.S. Department of Agriculture ; U.S. Department of Agriculture ; U.S. Department of Agriculture ; National Science Foundation ; National Science Foundation ; National Science Foundation ; 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National Basic Research Program of China ; National Basic Research Program of China ; U.S. Department of Agriculture ; U.S. Department of Agriculture ; U.S. Department of Agriculture ; U.S. Department of Agriculture ; U.S. Department of Agriculture ; U.S. Department of Agriculture ; U.S. Department of Agriculture ; U.S. Department of Agriculture ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; 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Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31172431] ; National Basic Research Program of China[2014CB138601] ; U.S. Department of Agriculture[USDA-NRI-2013-01107] ; National Science Foundation[NSF-IOS-1457282] ; National Institutes of Health[2R01GM085207-05]
WOS Research AreaImmunology ; Zoology
WOS SubjectImmunology ; Zoology
WOS IDWOS:000394635500011
WOS KeywordTELEOST FISH ; HEAVY-CHAIN ; 1ST IDENTIFICATION ; GENOME DUPLICATION ; ATLANTIC SALMON ; LYMPHOID ORGANS ; IMMUNE-SYSTEM ; B-CELLS ; EVOLUTION ; INSIGHTS
PublisherELSEVIER SCI LTD
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; U.S. Department of Agriculture ; U.S. Department of Agriculture ; U.S. Department of Agriculture ; U.S. Department of Agriculture ; U.S. Department of Agriculture ; U.S. Department of Agriculture ; U.S. Department of Agriculture ; U.S. Department of Agriculture ; 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Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/32913
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorSunyer, J. Oriol; Zhang, Yong-An
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
4.Univ Penn, Sch Vet Med, Dept Pathobiol, Philadelphia, PA 19104 USA
Recommended Citation
GB/T 7714
Zhang, Nu,Zhang, Xu-Jie,Chen, Dan-Dan,et al. Molecular characterization and expression analysis of three subclasses of IgT in rainbow trout (Oncorhynchus mykiss)[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2017,70(1):94-105.
APA Zhang, Nu,Zhang, Xu-Jie,Chen, Dan-Dan,Sunyer, J. Oriol,&Zhang, Yong-An.(2017).Molecular characterization and expression analysis of three subclasses of IgT in rainbow trout (Oncorhynchus mykiss).DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,70(1),94-105.
MLA Zhang, Nu,et al."Molecular characterization and expression analysis of three subclasses of IgT in rainbow trout (Oncorhynchus mykiss)".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 70.1(2017):94-105.
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