Grass Carp Reovirus VP41 Targets Fish MITA To Abrogate the Interferon Response
Lu, Long-Feng1,2; Li, Shun1,3; Wang, Zhao-Xi1,2; Du, Si-Qi4; Chen, Dan-Dan1,3; Nie, Pin1,3; Zhang, Yong-An1,3
2017-07-01
Source PublicationJOURNAL OF VIROLOGY
ISSN0022-538X
Volume91Issue:14
AbstractAlthough fish possess an efficient interferon (IFN) system to defend against aquatic virus infection, grass carp reovirus (GCRV) still causes hemorrhagic disease in grass carp. To date, GCRV's strategy for evading the fish IFN response is still unknown. Here, we report that GCRV VP41 inhibits fish IFN production by suppressing the phosphorylation of mediator of IFN regulatory factor 3 (IRF3) activation (MITA). First, the activation of the IFN promoter (IFNpro) stimulated by mitochondrial antiviral signaling protein (MAVS) and MITA was decreased by the overexpression of VP41, whereas such activation induced by TANK-binding kinase 1 (TBK1) was not affected. Second, VP41 was colocalized in the cellular endoplasmic reticulum (ER) and associated with MITA. Furthermore, as a phosphorylation substrate of TBK1, VP41 significantly decreased the phosphorylation of MITA. Truncation assays indicated that the transmembrane (TM) region of VP41 was indispensable for the suppression of IFNpro activity. Finally, after infection with GCRV, VP41 blunted the transcription of host IFN and facilitated viral RNA synthesis. Taken together, our findings suggest that GCRV VP41 prevents the fish IFN response by attenuating the phosphorylation of MITA for viral evasion.
SubtypeArticle
KeywordVp41 Gcrv Immune Evasion Mita Interferon
DOI10.1128/JVI.00390-17
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Basic Research Program of China ; National Basic Research Program of China ; CAS Major Scientific and Technological Project(2014CB138601 ; CAS Major Scientific and Technological Project(2014CB138601 ; National Natural Science Foundation of China(31502200) ; National Natural Science Foundation of China(31502200) ; XDA08010207) ; XDA08010207) ; National Basic Research Program of China ; National Basic Research Program of China ; CAS Major Scientific and Technological Project(2014CB138601 ; CAS Major Scientific and Technological Project(2014CB138601 ; National Natural Science Foundation of China(31502200) ; National Natural Science Foundation of China(31502200) ; XDA08010207) ; XDA08010207)
Language英语
WOS Research AreaVirology
WOS SubjectVirology
WOS KeywordHEPATITIS-C VIRUS ; ANTIVIRAL SIGNALING PROTEIN ; INNATE IMMUNE RECOGNITION ; COMPLETE GENOME SEQUENCE ; CYCLIC-DI-GMP ; RIG-I ; ADAPTER PROTEIN ; ENDOPLASMIC-RETICULUM ; REGULATORY FACTOR-3 ; ONCORHYNCHUS-MYKISS
WOS IDWOS:000405465700023
Funding OrganizationNational Basic Research Program of China ; National Basic Research Program of China ; CAS Major Scientific and Technological Project(2014CB138601 ; CAS Major Scientific and Technological Project(2014CB138601 ; National Natural Science Foundation of China(31502200) ; National Natural Science Foundation of China(31502200) ; XDA08010207) ; XDA08010207) ; National Basic Research Program of China ; National Basic Research Program of China ; CAS Major Scientific and Technological Project(2014CB138601 ; CAS Major Scientific and Technological Project(2014CB138601 ; National Natural Science Foundation of China(31502200) ; National Natural Science Foundation of China(31502200) ; XDA08010207) ; XDA08010207)
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Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/32859
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Minist Agr, Key Lab Aquaculture Dis Control, Wuhan, Peoples R China
4.Wuhan Univ, Coll Life Sci, Wuhan, Peoples R China
Recommended Citation
GB/T 7714
Lu, Long-Feng,Li, Shun,Wang, Zhao-Xi,et al. Grass Carp Reovirus VP41 Targets Fish MITA To Abrogate the Interferon Response[J]. JOURNAL OF VIROLOGY,2017,91(14).
APA Lu, Long-Feng.,Li, Shun.,Wang, Zhao-Xi.,Du, Si-Qi.,Chen, Dan-Dan.,...&Zhang, Yong-An.(2017).Grass Carp Reovirus VP41 Targets Fish MITA To Abrogate the Interferon Response.JOURNAL OF VIROLOGY,91(14).
MLA Lu, Long-Feng,et al."Grass Carp Reovirus VP41 Targets Fish MITA To Abrogate the Interferon Response".JOURNAL OF VIROLOGY 91.14(2017).
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