Quantitative Proteomics Analysis Reveals Novel Targets of miR-21 in Zebrafish Embryos
Wu, Ying1,2; Lou, Qi-Yong1; Ge, Feng1; Xiong, Qian1
2017-06-22
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
Volume7
AbstractMicroRNAs (miRNAs) are noncoding RNAs which control gene expression by the suppression of translation or the degradation of mRNAs. Dre-miR-21 (miR-21) has been reported to impact cardiac valvulogenesis in zebrafish embryos. However, the target genes of miR-21 are still largely unknown. Here a tandem isobaric mass tag (TMT)-based quantitative proteomic strategy was employed to identify the global profile of miR-21-regulated proteins. A total of 251 proteins were dysregulated after miR-21 knockdown, suggesting that they may be regulated by miR-21. Bioinformatics analysis indicated that these differentially expressed proteins (DEPs) participate in various biological processes, suggesting that miR-21 may be involved in diverse cellular pathways. Sixteen DEPs were also predicted to be miR-21 targets by at least two algorithms, and several candidate target genes were selected for further luciferase reporter analysis. The results showed that genes encoding tropomyosin 1 (tpm1) and poly(rC) binding protein 2 (pcbp2) are direct miR-21 targets. Taken together, our results not only reveal a large number of novel miR-21 regulated proteins that possess pleiotropic functions, but also provide novel insights into the molecular mechanisms of miR-21 regulation of zebrafish cardiac valvulogenesis and embryonic development.
SubtypeArticle
DOI10.1038/s41598-017-04166-x
WOS HeadingsScience & Technology
Indexed BySCI
Funding OrganizationNational Natural Science Foundation of China(21207153) ; National Natural Science Foundation of China(21207153) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14030202) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14030202) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2015FB10) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2015FB10) ; Youth Innovation Promotion Association of Chinese Academy of Sciences(2017387) ; Youth Innovation Promotion Association of Chinese Academy of Sciences(2017387) ; National Natural Science Foundation of China(21207153) ; National Natural Science Foundation of China(21207153) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14030202) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14030202) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2015FB10) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2015FB10) ; Youth Innovation Promotion Association of Chinese Academy of Sciences(2017387) ; Youth Innovation Promotion Association of Chinese Academy of Sciences(2017387)
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS KeywordTUMOR-SUPPRESSOR GENE ; HYPERTROPHIC CARDIOMYOPATHY ; INTERACTION NETWORKS ; POLIOVIRUS RNA ; HUMAN-CELLS ; EXPRESSION ; MICRORNA ; PROTEIN ; CANCER ; TROPOMYOSIN
WOS IDWOS:000403874900005
Funding OrganizationNational Natural Science Foundation of China(21207153) ; National Natural Science Foundation of China(21207153) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14030202) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14030202) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2015FB10) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2015FB10) ; Youth Innovation Promotion Association of Chinese Academy of Sciences(2017387) ; Youth Innovation Promotion Association of Chinese Academy of Sciences(2017387) ; National Natural Science Foundation of China(21207153) ; National Natural Science Foundation of China(21207153) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14030202) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14030202) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2015FB10) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2015FB10) ; Youth Innovation Promotion Association of Chinese Academy of Sciences(2017387) ; Youth Innovation Promotion Association of Chinese Academy of Sciences(2017387)
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/32858
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Wu, Ying,Lou, Qi-Yong,Ge, Feng,et al. Quantitative Proteomics Analysis Reveals Novel Targets of miR-21 in Zebrafish Embryos[J]. SCIENTIFIC REPORTS,2017,7.
APA Wu, Ying,Lou, Qi-Yong,Ge, Feng,&Xiong, Qian.(2017).Quantitative Proteomics Analysis Reveals Novel Targets of miR-21 in Zebrafish Embryos.SCIENTIFIC REPORTS,7.
MLA Wu, Ying,et al."Quantitative Proteomics Analysis Reveals Novel Targets of miR-21 in Zebrafish Embryos".SCIENTIFIC REPORTS 7(2017).
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