Dietary zinc deficiency reduced growth performance, intestinal immune and physical barrier functions related to NF-kappa B, TOR, Nrf2, JNK and MLCK signaling pathway of young grass carp (Ctenopharyngodon idella)
Song, Zheng-Xing1; Jiang, Wei-Dan1,2,3; Liu, Yang1,2,3; Wu, Pei1,2,3; Jiang, Jun1,2,3; Zhou, Xiao-Qiu1,2,3; Kuang, Sheng-Yao4; Tang, Ling4; Tang, Wu-Neng4; Zhang, Yong-An5; Feng, Lin1,2,3
2017-07-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume66Pages:497-523
AbstractOur study investigated the effects of dietary zinc (Zn) deficiency on growth performance, intestinal immune and physical barrier functions of young grass carp (Ctenopharyngodon idella). A total of 630 grass carp (244.14 +/- 0.40 g) were fed graded levels of zinc lactate (10.71, 30.21, 49.84, 7231, 92.56, 110.78 mg Zn/kg diet) and one zinc sulfate group (56.9 mg Zn/kg diet) for 60 days. At the end of the feeding trial, fish were challenged with Aeromonas hydrophila for 14 days. These results indicated that compared with optimal dietary Zn level, dietary Zn deficiency (10.71 mg/kg diet) decreased the production of antibacterial compounds, up-regulated pro-inflammatory cytokines related to nuclear factor kappa B (NF-kappa B) and down-regulated anti-inflammatory cytokines related to target of rapamycin (TOR) in three intestinal segments of young grass carp (P < 0.05), suggesting that dietary Zn deficiency could impair intestinal immune barrier of fish; decreased the activities and mRNA levels of antioxidant enzymes related to NF-E2-related factor 2 (Nrf2), up-regulated the mRNA levels of caspase-3,-7,-8,-9 related to p38 mitogen activated protein (p38 MAPK) and c-Jun N-terminal protein kinase (JNK), down-regulated the mRNA levels of tight junction complexes (TJs) related to myosin light chain kinase (MLCK) in three intestinal segments of young grass carp (P < 0.05), demonstrating that dietary Zn deficiency could injury intestinal physical barrier of fish. Besides, the Zn requirements (zinc lactate as Zn source) based on percent weight gain (PWG), against enteritis morbidity, acid phosphatase (ACP) activity in the proximal intestine (PI) and malondialdehyde (MDA) content in the PI of young grass carp was estimated to be 61.2, 61.4, 69.2 and 69.5 mg/kg diet, respectively. Finally, based on specific growth rate (SGR), feed efficiency (FE) and against enteritis morbidity of young grass. carp, the efficacy of zinc lactate relative to zinc sulfate were 132.59%, 135.27% and 154.04%, respectively. (C) 2017 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordZinc Grass Carp (Ctenopharyngodon Idella) Intestinal Immune Barrier Physical Barrier Efficacy
DOI10.1016/j.fsi.2017.05.048
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; Outstanding Talents and Innovative Team of Agricultural Scientific Research (Ministry of Agriculture) ; Outstanding Talents and Innovative Team of Agricultural Scientific Research (Ministry of Agriculture) ; National Natural Science Foundation of China(31672664) ; National Natural Science Foundation of China(31672664) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; Specialized Research Fund for the Doctoral Program of Higher Education Commission, Pakistan(20135103110001) ; Specialized Research Fund for the Doctoral Program of Higher Education Commission, Pakistan(20135103110001) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; 2013NC0045) ; 2013NC0045) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; Outstanding Talents and Innovative Team of Agricultural Scientific Research (Ministry of Agriculture) ; Outstanding Talents and Innovative Team of Agricultural Scientific Research (Ministry of Agriculture) ; National Natural Science Foundation of China(31672664) ; National Natural Science Foundation of China(31672664) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; Specialized Research Fund for the Doctoral Program of Higher Education Commission, Pakistan(20135103110001) ; Specialized Research Fund for the Doctoral Program of Higher Education Commission, Pakistan(20135103110001) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; 2013NC0045) ; 2013NC0045)
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS KeywordTROUT ONCORHYNCHUS-MYKISS ; PACIFIC WHITE SHRIMP ; MESSENGER-RNA LEVELS ; SALMON SALMO-SALAR ; SPARUS-AURATA L. ; OXIDATIVE STRESS ; GENE-EXPRESSION ; JUVENILE JIAN ; VAR. JIAN ; ANTIOXIDANT STATUS
WOS IDWOS:000403986100054
Funding OrganizationNational Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; Outstanding Talents and Innovative Team of Agricultural Scientific Research (Ministry of Agriculture) ; Outstanding Talents and Innovative Team of Agricultural Scientific Research (Ministry of Agriculture) ; National Natural Science Foundation of China(31672664) ; National Natural Science Foundation of China(31672664) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; Specialized Research Fund for the Doctoral Program of Higher Education Commission, Pakistan(20135103110001) ; Specialized Research Fund for the Doctoral Program of Higher Education Commission, Pakistan(20135103110001) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; 2013NC0045) ; 2013NC0045) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; Outstanding Talents and Innovative Team of Agricultural Scientific Research (Ministry of Agriculture) ; Outstanding Talents and Innovative Team of Agricultural Scientific Research (Ministry of Agriculture) ; National Natural Science Foundation of China(31672664) ; National Natural Science Foundation of China(31672664) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; Specialized Research Fund for the Doctoral Program of Higher Education Commission, Pakistan(20135103110001) ; Specialized Research Fund for the Doctoral Program of Higher Education Commission, Pakistan(20135103110001) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; 2013NC0045) ; 2013NC0045)
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/32839
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Affiliation1.Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China
2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov, Chengdu 611130, Sichuan, Peoples R China
3.Sichuan Agr Univ, Key Lab Anim Dis Resistance Nutr, China Minist Educ, Chengdu 611130, Sichuan, Peoples R China
4.Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 610066, Peoples R China
5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Song, Zheng-Xing,Jiang, Wei-Dan,Liu, Yang,et al. Dietary zinc deficiency reduced growth performance, intestinal immune and physical barrier functions related to NF-kappa B, TOR, Nrf2, JNK and MLCK signaling pathway of young grass carp (Ctenopharyngodon idella)[J]. FISH & SHELLFISH IMMUNOLOGY,2017,66:497-523.
APA Song, Zheng-Xing.,Jiang, Wei-Dan.,Liu, Yang.,Wu, Pei.,Jiang, Jun.,...&Feng, Lin.(2017).Dietary zinc deficiency reduced growth performance, intestinal immune and physical barrier functions related to NF-kappa B, TOR, Nrf2, JNK and MLCK signaling pathway of young grass carp (Ctenopharyngodon idella).FISH & SHELLFISH IMMUNOLOGY,66,497-523.
MLA Song, Zheng-Xing,et al."Dietary zinc deficiency reduced growth performance, intestinal immune and physical barrier functions related to NF-kappa B, TOR, Nrf2, JNK and MLCK signaling pathway of young grass carp (Ctenopharyngodon idella)".FISH & SHELLFISH IMMUNOLOGY 66(2017):497-523.
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