IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
The construction of intrahepatic cholangiocarcinoma model in zebrafish
Wang, Jing1; Leng, Xiaoqian1,2; Wang, Guiping1; Wan, Xiaoyang1; Cao, Hong1
2017-10-17
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
Volume7Issue:1Pages:11
Abstract

Intrahepatic cholangiocarcinoma (ICC) is a highly malignant tumor, difficult to diagnose even at an early stage. In this study, we successfully constructed an nras(61K)-induced ICC model in zebrafish. Transcriptome analysis and gene set enrichment analysis (GSEA) of liver samples of the ICC and WT (wild-type) zebrafish revealed that the genes differentially expressed between the two groups were mainly involved in focal adhesion, chemokine signaling and metabolic pathways. Analysis of DNA methylomes revealed that compared with WT samples, methylated genes in ICC samples were enriched in functions associated with cellular, single-organism and metabolic processes. In particular, our result discovered eleven potential biomarker genes of ICC which were conserved between zebrafish and humans. Moreover, three potential biomarker genes were hypomethylated in the tumorigenesis of ICC: ehf, epha4 and itgb6. In summary, our study provides a comprehensive analysis of molecular mechanisms accompanying the progressive nras61K-induced ICC. This work indicates that our transgenic zebrafish could be a valuable model, not only for studying liver cancer, but also for exploring new therapeutic targets.

DOI10.1038/s41598-017-13815-0
Funding OrganizationHi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
Indexed BySCI
Language英语
Funding ProjectHi-Tech Research and Development Program of China (863 Program)[2012AA022402-4] ; National Natural Science Foundation of China[31440080]
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000413084800116
WOS KeywordEPITHELIAL-MESENCHYMAL TRANSITION ; HEPATOCELLULAR-CARCINOMA ; TRANSGENIC ZEBRAFISH ; GENE-EXPRESSION ; GENOME-WIDE ; DNA HYPOMETHYLATION ; BREAST-CANCER ; METHYLATION ; RAS ; TUMORIGENESIS
PublisherNATURE PUBLISHING GROUP
Funding OrganizationHi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; Hi-Tech Research and Development Program of China (863 Program) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/32656
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorCao, Hong
Affiliation1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Fisheries Sci, Minist Agr China, Key Lab Freshwater Biodivers Conservat, Wuhan 430223, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Wang, Jing,Leng, Xiaoqian,Wang, Guiping,et al. The construction of intrahepatic cholangiocarcinoma model in zebrafish[J]. SCIENTIFIC REPORTS,2017,7(1):11.
APA Wang, Jing,Leng, Xiaoqian,Wang, Guiping,Wan, Xiaoyang,&Cao, Hong.(2017).The construction of intrahepatic cholangiocarcinoma model in zebrafish.SCIENTIFIC REPORTS,7(1),11.
MLA Wang, Jing,et al."The construction of intrahepatic cholangiocarcinoma model in zebrafish".SCIENTIFIC REPORTS 7.1(2017):11.
Files in This Item: Download All
File Name/Size DocType Version Access License
s41598-017-13815-0.p(2879KB)期刊论文出版稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, Jing]'s Articles
[Leng, Xiaoqian]'s Articles
[Wang, Guiping]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Jing]'s Articles
[Leng, Xiaoqian]'s Articles
[Wang, Guiping]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Jing]'s Articles
[Leng, Xiaoqian]'s Articles
[Wang, Guiping]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: s41598-017-13815-0.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.