IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Molecular cloning, biological effect, and tissue distribution of interleukin-8 protein in mandarin fish (Siniperca chuasti) upon Flavobacterium columnare infection
Wang, Gai Ling1,2; Wang, Ming Cheng2; Zhang, Xiao Wen1; Chang, Ming Xian1; Xie, Hai Xia1; Nie, Pin1
2017-07-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume66Issue:1Pages:112-119
Abstract

Interleukin-8 (IL-8), a CXC-type chemokine, plays a key role in acute inflammation by recruiting neutrophils in mammals. In the present study, the open reading frame (ORF) of IL-8, encoding 99 amino acids was cloned in mandarin fish, and its function in inflammation was investigated. The IL-8 contains four conserved cysteine residues, with the first two forming the CXC signature motif. The genomic sequence of mandarin fish IL-8 has four exons and three introns, a typical gene organization of the CXC chemokine. Bioactive recombinant IL-8 (rIL-8) exhibited a,chemotactic effect on head kidney leukocytes in vitro, and activates the transcription of the inflammatory genes, IL-8 and IL-10. When mandarin fish was challenged intraperitoneally with the pathogenic bacterium Flavobacterium columnare G(4), the steady-state protein level of IL-8 was up-regulated in trunk kidney and head kidney. These results suggest that IL-8 is a functional CXC chemokine in mandarin fish, and plays a key role in the inflammatory responses towards bacterial infection. (C) 2017 Elsevier Ltd. All rights reserved.

KeywordInterleuldn-8 Biological effect Tissue distribution Mandarin fish (Siniperca chuatsi)
DOI10.1016/j.fsi.2017.05.016
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31230075] ; National Natural Science Foundation of China[31402334] ; Foundation and Advanced Technology Research Project of Henan Province[142300410111]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000403986100013
WOS KeywordTROUT ONCORHYNCHUS-MYKISS ; PRO-INFLAMMATORY CYTOKINES ; EXPRESSION ANALYSIS ; RECEPTOR ; CHEMOKINE ; GENE ; IL-8 ; IL-1-BETA ; CXCL8 ; STIMULATION
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province ; Foundation and Advanced Technology Research Project of Henan Province
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/32441
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorXie, Hai Xia
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
2.Huanghuai Univ, Sch Biol & Food Proc Engn, Zhumadian 463000, Henan Province, Peoples R China
Recommended Citation
GB/T 7714
Wang, Gai Ling,Wang, Ming Cheng,Zhang, Xiao Wen,et al. Molecular cloning, biological effect, and tissue distribution of interleukin-8 protein in mandarin fish (Siniperca chuasti) upon Flavobacterium columnare infection[J]. FISH & SHELLFISH IMMUNOLOGY,2017,66(1):112-119.
APA Wang, Gai Ling,Wang, Ming Cheng,Zhang, Xiao Wen,Chang, Ming Xian,Xie, Hai Xia,&Nie, Pin.(2017).Molecular cloning, biological effect, and tissue distribution of interleukin-8 protein in mandarin fish (Siniperca chuasti) upon Flavobacterium columnare infection.FISH & SHELLFISH IMMUNOLOGY,66(1),112-119.
MLA Wang, Gai Ling,et al."Molecular cloning, biological effect, and tissue distribution of interleukin-8 protein in mandarin fish (Siniperca chuasti) upon Flavobacterium columnare infection".FISH & SHELLFISH IMMUNOLOGY 66.1(2017):112-119.
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