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Comparative metabolic profiling of the lipid-producing green microalga Chlorella reveals that nitrogen and carbon metabolic pathways contribute to lipid metabolism
Chen, Hui1; Zheng, Yanli1,2; Zhan, Jiao1; He, Chenliu1; Wang, Qiang3
2017-06-15
Source PublicationBIOTECHNOLOGY FOR BIOFUELS
ISSN1754-6834
Volume10Issue:1Pages:20
Abstract

Microalgae are a promising feedstock for biofuel production. Microalgal metabolic pathways are heavily influenced by environmental factors. For instance, lipid metabolism can be induced by nitrogen-limiting conditions. However, the underlying mechanisms of lipid biosynthesis are unclear. In this study, we analyzed the global metabolic profiles of three genetically closely related Chlorella strains (C1, C2, and C3) with significant differences in lipid productivity to identify the contributions of key metabolic pathways to lipid metabolism. We found that nitrogen obtained from amino acid catabolism was assimilated via the glutamate-glutamine pathway and then stored as amino acids and intermediate molecules (particularly proline, alanine, arginine, succinate, and gamma-aminobutyrate) via the corresponding metabolic pathways, which led to carbon-nitrogen disequilibrium. Excess carbon obtained from photosynthesis or glycolysis was re-distributed into carbon-containing compounds, such as glucose-6-phosphate, fructose6-phosphate, phosphoenolpyruvate, lactate, citrate, 3-hydroxybutyrate, and leucine, and then diverted into lipid metabolism for the production of storage lipids via the gamma-aminobutyrate pathway, glycolysis, and the tricarboxylic acid cycle. These results were substantiated in the model green alga Chlamydomonas reinhardtii by analyzing various mutants deficient in glutamate synthase/NADH-dependent, glutamate synthase/Fd-dependent, glutamine synthetase, aspartate aminotransferase, alanine aminotransferase, pyruvate kinase, and citrate synthase. Our study suggests that not only carbon but also nitrogen assimilation and distribution pathways contribute to lipid biosynthesis. Furthermore, these findings may facilitate genetic engineering efforts to enhance microalgal biofuel production.

KeywordCarbon metabolism Lipid metabolism Metabolome Microalgae Nitrogen assimilation Nitrogen salvage
DOI10.1186/s13068-017-0839-4
Funding OrganizationState Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS
Indexed BySCI
Language英语
Funding ProjectState Key Laboratory of Freshwater Ecology and Biotechnology[Y11901-1-F01] ; Science and Technology Service Network Initiative of the CAS[KFJ-SW-STS-163]
WOS Research AreaBiotechnology & Applied Microbiology ; Energy & Fuels
WOS SubjectBiotechnology & Applied Microbiology ; Energy & Fuels
WOS IDWOS:000403320100001
WOS KeywordDIATOM PHAEODACTYLUM-TRICORNUTUM ; GLUTAMATE SYNTHASE ACTIVITIES ; INDUSTRIAL FLUE-GASES ; ALGA CHLORELLA ; CHLAMYDOMONAS-REINHARDTII ; AMINO-ACID ; SP C2 ; ACCUMULATION ; SYNTHETASE ; PROTEIN
PublisherBIOMED CENTRAL LTD
Funding OrganizationState Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS ; Science and Technology Service Network Initiative of the CAS
Citation statistics
Cited Times:29[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/32432
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorWang, Qiang
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Chen, Hui,Zheng, Yanli,Zhan, Jiao,et al. Comparative metabolic profiling of the lipid-producing green microalga Chlorella reveals that nitrogen and carbon metabolic pathways contribute to lipid metabolism[J]. BIOTECHNOLOGY FOR BIOFUELS,2017,10(1):20.
APA Chen, Hui,Zheng, Yanli,Zhan, Jiao,He, Chenliu,&Wang, Qiang.(2017).Comparative metabolic profiling of the lipid-producing green microalga Chlorella reveals that nitrogen and carbon metabolic pathways contribute to lipid metabolism.BIOTECHNOLOGY FOR BIOFUELS,10(1),20.
MLA Chen, Hui,et al."Comparative metabolic profiling of the lipid-producing green microalga Chlorella reveals that nitrogen and carbon metabolic pathways contribute to lipid metabolism".BIOTECHNOLOGY FOR BIOFUELS 10.1(2017):20.
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