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Genetic analyses unravel the crucial role of a horizontally acquired alginate lyase for brown algal biomass degradation by Zobellia galactanivorans
Zhu, Yongtao1; Thomas, Francois2; Larocque, Robert2; Li, Nan3; Duffieux, Delphine2; Cladiere, Lionel2; Souchaud, Florent2; Michel, Gurvan2; McBride, Mark J.1
2017-06-01
Source PublicationENVIRONMENTAL MICROBIOLOGY
ISSN1462-2912
Volume19Issue:6Pages:2164-2181
Abstract

Comprehension of the degradation of macroalgal polysaccharides suffers from the lack of genetic tools for model marine bacteria, despite their importance for coastal ecosystem functions. We developed such tools for Zobellia galactanivorans, an algaeassociated flavobacterium that digests many polysaccharides, including alginate. These tools were used to investigate the biological role of AlyA1, the only Z. galactanivorans alginate lyase known to be secreted in soluble form and to have a recognizable carbohydrate-binding domain. A deletion mutant, DalyA1, grew as well as the wild type on soluble alginate but was deficient in soluble secreted alginate lyase activity and in digestion of and growth on alginate gels and algal tissues. Thus, AlyA1 appears to be essential for optimal attack of alginate in intact cell walls. alyA1 appears to have been recently acquired via horizontal transfer from marine Actinobacteria, conferring an adaptive advantage that might benefit other algae-associated bacteria by exposing new substrate niches. The genetic tools described here function in diverse members of the phylum Bacteroidetes and should facilitate analyses of polysaccharide degradation systems and many other processes in these common but understudied bacteria.

DOI10.1111/1462-2920.13699
Funding OrganizationNational Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency
Indexed BySCI
Language英语
Funding ProjectNational Science Foundation[MCB-1516990] ; French National Research Agency[ANR-14-CE19-0020-01]
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000404007700009
WOS KeywordJOHNSONIAE GLIDING MOTILITY ; CYTOPHAGA-HUTCHINSONII ; FLAVOBACTERIUM-JOHNSONIAE ; ESCHERICHIA-COLI ; CELL-WALLS ; STRUCTURAL-CHARACTERIZATION ; BACTERIAL COMMUNITIES ; DELETION STRATEGY ; MARINE-BACTERIA ; GUT MICROBIOTA
PublisherWILEY
Funding OrganizationNational Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency ; French National Research Agency
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/32404
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorMichel, Gurvan; McBride, Mark J.
Affiliation1.Univ Wisconsin, Dept Biol Sci, POB 413, Milwaukee, WI 53201 USA
2.Sorbonne Univ, UPMC, CNRS, Integrat Biol Marine Models,UMR 8227,Stn Biol Ros, Roscoff, France
3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
Recommended Citation
GB/T 7714
Zhu, Yongtao,Thomas, Francois,Larocque, Robert,et al. Genetic analyses unravel the crucial role of a horizontally acquired alginate lyase for brown algal biomass degradation by Zobellia galactanivorans[J]. ENVIRONMENTAL MICROBIOLOGY,2017,19(6):2164-2181.
APA Zhu, Yongtao.,Thomas, Francois.,Larocque, Robert.,Li, Nan.,Duffieux, Delphine.,...&McBride, Mark J..(2017).Genetic analyses unravel the crucial role of a horizontally acquired alginate lyase for brown algal biomass degradation by Zobellia galactanivorans.ENVIRONMENTAL MICROBIOLOGY,19(6),2164-2181.
MLA Zhu, Yongtao,et al."Genetic analyses unravel the crucial role of a horizontally acquired alginate lyase for brown algal biomass degradation by Zobellia galactanivorans".ENVIRONMENTAL MICROBIOLOGY 19.6(2017):2164-2181.
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