IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Differences in responses of grass carp to different types of grass carp reovirus (GCRV) and the mechanism of hemorrhage revealed by transcriptome sequencing
He, Libo1; Zhang, Aidi1; Pei, Yongyan1,2; Chu, Pengfei1,2; Li, Yongming1; Huang, Rong1; Liao, Lanjie1; Zhu, Zuoyan1; Wang, Yaping1
Corresponding AuthorWang, Yaping(wangyp@ihb.ac.cn)
2017-06-08
Source PublicationBMC GENOMICS
ISSN1471-2164
Volume18Pages:15
AbstractBackground: Grass carp is an important farmed fish in China that is affected by serious disease, especially hemorrhagic disease caused by grass carp reovirus (GCRV). The mechanism underlying the hemorrhagic symptoms in infected fish remains to be elucidated. Although GCRV can be divided into three distinct subtypes, differences in the pathogenesis and host immune responses to the different subtypes are still unclear. The aim of this study was to provide a comprehensive insight into the grass carp response to different GCRV subtypes and to elucidate the mechanism underlying the hemorrhagic symptoms. Results: Following infection of grass carp, GCRV-I was associated with a long latent period and low mortality (42.5%), while GCRV-II was associated with a short latent period and high mortality (81.4%). The relative copy number of GCRV-I remained consistent or decreased slightly throughout the first 7 days post-infection, whereas a marked increase in GCRV-II high copy number was detected at 5 days post-infection. Transcriptome sequencing revealed 211 differentially expressed genes (DEGs) in Group I (66 up-regulated, 145 down-regulated) and 670 (386 up-regulated, 284 down-regulated) in Group II. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed significant enrichment in the terms or pathways involved in immune responses and correlating with blood or platelets. Most of the DEGs in Group I were also present in Group II, although the expression profiles differed, with most DEGs showing mild changes in Group I, while marked changes were observed in Group II, especially the interferon-related genes. Many of the genes involved in the complement pathway and coagulation cascades were significantly up-regulated at 7 days post-infection in Group II, suggesting activation of these pathways. Conclusion: GCRV-I is associated with low virulence and a long latent period prior to the induction of a mild host immune response, whereas GCRV-II is associated with high virulence, a short latent period and stimulates a strong and extensive host immune response. The complement and coagulation pathways are significantly activated at 7 days post-infection, leading to the endothelial cell and blood cell damage that result in hemorrhagic symptoms.
KeywordGrass carp Grass carp reovirus Transcriptome sequencing Immune response Hemorrhage
DOI10.1186/s12864-017-3824-1
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences
Indexed BySCI ; SCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31130055] ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology[2014FBZ04] ; Knowledge Innovation Program of the Chinese Academy of Sciences[KSCX2-EW-N-004-3] ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences[Y65E021301]
WOS Research AreaBiotechnology & Applied Microbiology ; Genetics & Heredity
WOS SubjectBiotechnology & Applied Microbiology ; Genetics & Heredity
WOS IDWOS:000404081100003
WOS KeywordHEMATOPOIETIC NECROSIS VIRUS ; COMPLETE GENOME SEQUENCE ; INNATE IMMUNE-RESPONSE ; PROKARYOTIC EXPRESSION ; COMPLEMENT ACTIVATION ; RNA-SEQ ; FISH ; GENE ; INFECTION ; PATHWAY
PublisherBIOMED CENTRAL LTD
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/32361
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorWang, Yaping
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
He, Libo,Zhang, Aidi,Pei, Yongyan,et al. Differences in responses of grass carp to different types of grass carp reovirus (GCRV) and the mechanism of hemorrhage revealed by transcriptome sequencing[J]. BMC GENOMICS,2017,18:15.
APA He, Libo.,Zhang, Aidi.,Pei, Yongyan.,Chu, Pengfei.,Li, Yongming.,...&Wang, Yaping.(2017).Differences in responses of grass carp to different types of grass carp reovirus (GCRV) and the mechanism of hemorrhage revealed by transcriptome sequencing.BMC GENOMICS,18,15.
MLA He, Libo,et al."Differences in responses of grass carp to different types of grass carp reovirus (GCRV) and the mechanism of hemorrhage revealed by transcriptome sequencing".BMC GENOMICS 18(2017):15.
Files in This Item: Download All
File Name/Size DocType Version Access License
s12864-017-3824-1.pd(2079KB)期刊论文出版稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[He, Libo]'s Articles
[Zhang, Aidi]'s Articles
[Pei, Yongyan]'s Articles
Baidu academic
Similar articles in Baidu academic
[He, Libo]'s Articles
[Zhang, Aidi]'s Articles
[Pei, Yongyan]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[He, Libo]'s Articles
[Zhang, Aidi]'s Articles
[Pei, Yongyan]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: s12864-017-3824-1.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.