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3-Hydroxyisobutyryl-CoA hydrolase involved in isoleucine catabolism regulates triacylglycerol accumulation in Phaeodactylum tricornutum
Pan, Yufang1; Yang, Juan1,2; Gong, Yangmin3; Li, Xiaolong1; Hu, Hanhua1
2017-09-05
Source PublicationPHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
ISSN0962-8436
Volume372Issue:1728Pages:10
Abstract

Since methylmalonyl-CoA epimerase appears to be absent in the majority of photosynthetic organisms, including diatoms, (S)-methylmalonyl-CoA, the intermediate of isoleucine (Ile) catabolism, cannot be metabolized to (R)methylmalonyl-CoA then to succinyl-CoA. In this study, propionyl-CoA carboxylase (PCC) RNAi silenced strains and 3-hydroxyisobutyryl-CoA hydrolase (HIBCH) overexpression strains were constructed to elucidate the Ile degradation pathway and its influence on lipid accumulation in Phaeodactylum tricornutum based on growth, neutral lipid content and metabolite profile analysis. Knockdown of PCC disturbed the metabolism of Ile through propionyl-CoA to methylmalonyl-CoA, as illustrated by much higher Ile content at day 6. However, Ile decreased to comparable levels to the wild-type at day 10. PCC silencing redirected propionyl-CoA to acetyl-CoA via a modified beta-oxidation pathway, and transcript levels for some branched-chain amino acid (BCAA) degradation-related genes, especially HIBCH, significantly upregulated in the PCC mutant, which enhanced the BCAA degradations and thus resulted in higher triacylglycerol (TAG) content. Overexpression of HIBCH accelerates Ile degradation and results in a lowered Ile content in the overexpression strains, thus enhancing carbon skeletons to the tricarboxylic acid cycle and giving rise to increasing TAG accumulation. Our study provides a good strategy to obtain high-lipid-yield transgenic diatoms by modifying the propionyl-CoA metabolism. This article is part of the themed issue 'The peculiar carbon metabolism in diatoms'.

Keyworddiatom propionyl-CoA carboxylase branched-chain amino acid catabolism beta-oxidation
DOI10.1098/rstb.2016.0409
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[41576144] ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology[2015FB12]
WOS Research AreaLife Sciences & Biomedicine - Other Topics
WOS SubjectBiology
WOS IDWOS:000405676200014
WOS KeywordARABIDOPSIS-THALIANA ; COMPARATIVE GENOMICS ; BETA-OXIDATION ; UREA CYCLE ; DIATOM ; METABOLISM ; EVOLUTION ; COA ; CARBOXYLASE ; COENZYME
PublisherROYAL SOC
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/32305
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorHu, Hanhua
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Agr Sci, Oil Crops Res Inst, Minist Agr, Key Lab Biol & Genet Improvement Oil Crops, Wuhan 430062, Peoples R China
Recommended Citation
GB/T 7714
Pan, Yufang,Yang, Juan,Gong, Yangmin,et al. 3-Hydroxyisobutyryl-CoA hydrolase involved in isoleucine catabolism regulates triacylglycerol accumulation in Phaeodactylum tricornutum[J]. PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES,2017,372(1728):10.
APA Pan, Yufang,Yang, Juan,Gong, Yangmin,Li, Xiaolong,&Hu, Hanhua.(2017).3-Hydroxyisobutyryl-CoA hydrolase involved in isoleucine catabolism regulates triacylglycerol accumulation in Phaeodactylum tricornutum.PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES,372(1728),10.
MLA Pan, Yufang,et al."3-Hydroxyisobutyryl-CoA hydrolase involved in isoleucine catabolism regulates triacylglycerol accumulation in Phaeodactylum tricornutum".PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES 372.1728(2017):10.
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