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G beta 1 is required for neutrophil migration in zebrafish
Ke, Wenfan1; Ye, Ding1,3; Mersch, Kacey1; Xu, Hui1,4; Chen, Songhai2; Lin, Fang1,5
2017-08-01
Source PublicationDEVELOPMENTAL BIOLOGY
ISSN0012-1606
Volume428Issue:1Pages:135-147
Abstract

Signaling mediated by G protein-coupled receptors (GPCRs) is essential for the migration of cells toward chemoattractants. The recruitment of neutrophils to injured tissues in zebrafish larvae is a useful model for studying neutrophil migration and trafficking in vivo. Indeed, the study of this process led to the discovery that PI3Ky is required for the polarity and motility of neutrophils, features that are necessary for the directed migration of these cells to wounds. However, the mechanism by which PI3Ky is activated remains to be determined. Here we show that signaling by specifically the heterotrimeric G protein subunit G beta 1 is critical for neutrophil migration in response to wounding. In embryos treated with small-molecule inhibitors of G beta y signaling, neutrophils failed to migrate to wound sites. Although both the G beta 1 and G beta 4 isoforms are expressed in migrating neutrophils, only deficiency for the former (morpholino-based knockdown) interfered with the directed migration of neutrophils towards wounds. The G beta 1 deficiency also impaired the ability of cells to change cell shape and reduced their general motility, defects that are similar to those in neutrophils deficient for PI3Ky. Transplantation assays showed that the requirement for G beta 1 in neutrophil migration is cell autonomous. Finally, live imaging revealed that G beta 1 is required for polarized activation of PI3K, and for the actin dynamics that enable neutrophil migration. Collectively, our data indicate that Gill signaling controls proper neutrophil migration by activating PI3K and modulating actin dynamics. Moreover, they illustrate a role for a specific G beta isoform in chemotaxis in vivo.

KeywordCell migration Neutrophil G protein G beta 1 Imaging
DOI10.1016/j.ydbio.2017.05.024
Funding OrganizationNIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH
Indexed BySCI
Language英语
Funding ProjectNIH/NCRR[K99R00RR024119] ; National Science Foundation[IOS-1354457] ; NIH[R01GM094255] ; [AHA10GRNT3620015]
WOS Research AreaDevelopmental Biology
WOS SubjectDevelopmental Biology
WOS IDWOS:000406255300010
WOS KeywordBETA-GAMMA-SUBUNITS ; SIGNALING CONTROLS ; CELL-MIGRATION ; TRANSGENIC ZEBRAFISH ; BACTERIAL-INFECTION ; ACTIN CYTOSKELETON ; LATERAL-LINE ; GASTRULATION ; CHEMOTAXIS ; RECEPTOR
PublisherACADEMIC PRESS INC ELSEVIER SCIENCE
Funding OrganizationNIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; NIH/NCRR ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH ; NIH
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/32249
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorLin, Fang
Affiliation1.Univ Iowa, Carver Coll Med, Dept Anat & Cell Biol, Iowa City, IA 52242 USA
2.Univ Iowa, Dept Pharmacol, Carver Coll Med, Iowa City, IA 52242 USA
3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
4.Nantong Univ, Jiangsu Key Lab Neural Regenerat, Med Sch, Nantong 226001, Jiangsu, Peoples R China
5.Univ Virginia, Dept Biol, Charlottesville, VA 22903 USA
Recommended Citation
GB/T 7714
Ke, Wenfan,Ye, Ding,Mersch, Kacey,et al. G beta 1 is required for neutrophil migration in zebrafish[J]. DEVELOPMENTAL BIOLOGY,2017,428(1):135-147.
APA Ke, Wenfan,Ye, Ding,Mersch, Kacey,Xu, Hui,Chen, Songhai,&Lin, Fang.(2017).G beta 1 is required for neutrophil migration in zebrafish.DEVELOPMENTAL BIOLOGY,428(1),135-147.
MLA Ke, Wenfan,et al."G beta 1 is required for neutrophil migration in zebrafish".DEVELOPMENTAL BIOLOGY 428.1(2017):135-147.
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