IHB OpenIR  > 藻类生物技术和生物能源研发中心  > 期刊论文
Metabolic plasticity of the starchless mutant of Chlorella sorokiniana and mechanisms underlying its enhanced lipid production revealed by comparative metabolomics analysis
Wu, Mingcan1; Zhang, Hu2,6; Sun, Wenchao2; Li, Yanhua2; Hu, Qiang2,3,4,7,8; Zhou, Hantao1; Han, Danxiang2,5
2019-09-01
Source PublicationALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
ISSN2211-9264
Volume42Issue:1Pages:13
Abstract

The unicellular green alga Chlorella sorokiniana is of great industrial interest for biofuels production due to its rapid growth, high lipid content, and capabilities in tolerating broad environment and cultivation conditions. C. sorokiniana can grow under both heterotrophic and photoautotrophic conditions, which offers opportunities to produce lipids from this species/strain by employing coupled heterotrophic and phototrophic cultivation mode. In this study, a starchless mutant SLM2 was successfully obtained by high-throughput screening of the chemical mutagenesis pool of a C. sorokiniana strain that has the potential to achieve high cell density under heterotrophic conditions, aiming to enhance its lipid content and productivity. Unexpectedly, enhanced lipid production was only observed in photoautrophically-grown SLM2 cells subjected to stress conditions (i.e. high-light and nutrient limitation). When the heterotrophically-grown cells were subjected to the same stress conditions that can trigger lipid accumulation, less differences in lipid productivities were observed between wild type (WT) and SLM2. To understand metabolic basis underlying such a discrepancy, comparative time-course metabolomics analysis was performed for SLM2 and WT from different cultivation conditions. The results highlighted up-regulated metabolic flux related to the increased lipid biosynthesis in the phototrophically-grown starchless mutant cells, which included enhanced oxidative pentose phosphate pathway in a mode favoring NADPH production, and the augmented glycolysis pathway providing precursors for both fatty acids and glycerol backbone synthesis. In SLM2, the classical TCA cycle is down-regulated, while its bypass the gamma-aminobutyric acid (GABA) shunt is triggered, which is speculated to contribute to enhanced lipid accumulation in the starchless mutant as well. In addition to revealing the metabolic plasticity possessed by starchless mutant, it is suggested that photoautotrophic cultivation mode is more suitable than coupled heterotrophic and photoautotrophic cultivation mode for production of lipids by using the starchless mutant SLM2 obtained in this study.

KeywordMicroalgae Chlorella sorokiniana Lipid production Starchless mutant Metabolome
DOI10.1016/j.algal.2019.101587
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31371988] ; Ministry of Education Science and Technology Research Project[113039A] ; State Development and Investment Corporation[IHB/CN/2014033]
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000478787200026
WOS KeywordCHLAMYDOMONAS-REINHARDTII ; GROWTH-RATE ; CARBON METABOLISM ; ACCUMULATION ; MICROALGA ; CULTIVATION ; STRATEGY ; CULTURE ; BIOMASS ; HEAT
PublisherELSEVIER
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; Ministry of Education Science and Technology Research Project ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation ; State Development and Investment Corporation
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/32089
Collection藻类生物技术和生物能源研发中心_期刊论文
Corresponding AuthorZhou, Hantao; Han, Danxiang
Affiliation1.Xiamen Univ, State Key Lab Marine Environm Sci, Coll Ocean & Earth Sci, Xiamen 361102, Fujian, Peoples R China
2.Chinese Acad Sci, Ctr Microalgal Biotechnol & Biofuels, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
5.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China
6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
7.SDIC Biotechnol Investment Co Ltd, Shanghai 200080, Peoples R China
8.Beijing Key Lab Algae Biomass, Beijing 100142, Peoples R China
Recommended Citation
GB/T 7714
Wu, Mingcan,Zhang, Hu,Sun, Wenchao,et al. Metabolic plasticity of the starchless mutant of Chlorella sorokiniana and mechanisms underlying its enhanced lipid production revealed by comparative metabolomics analysis[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2019,42(1):13.
APA Wu, Mingcan.,Zhang, Hu.,Sun, Wenchao.,Li, Yanhua.,Hu, Qiang.,...&Han, Danxiang.(2019).Metabolic plasticity of the starchless mutant of Chlorella sorokiniana and mechanisms underlying its enhanced lipid production revealed by comparative metabolomics analysis.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,42(1),13.
MLA Wu, Mingcan,et al."Metabolic plasticity of the starchless mutant of Chlorella sorokiniana and mechanisms underlying its enhanced lipid production revealed by comparative metabolomics analysis".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 42.1(2019):13.
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