IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
The P Protein of Spring Viremia of Carp Virus Negatively Regulates the Fish Interferon Response by Inhibiting the Kinase Activity of TANK-Binding Kinase 1
Li, Shun1; Lu, Long-Feng1,2; Wang, Zhao-Xi1,2; Lu, Xiao-Bing1,2; Chen, Dan-Dan1; Nie, Pin1; Zhang, Yong-An1
2016-12-01
Source PublicationJOURNAL OF VIROLOGY
ISSN0022-538X
Volume90Issue:23Pages:10728-10737
Abstract

Spring viremia of carp virus (SVCV) is an efficient pathogen causing high mortality in the common carp. Fish interferon (IFN) is a powerful cytokine enabling host cells to establish an antiviral response; therefore, the strategies that SVCV uses to avoid the cellular IFN response were investigated. Here, we report that the SVCV P protein is phosphorylated by cellular TANK-binding kinase 1 (TBK1), which decreases IFN regulatory factor 3 (IRF3) phosphorylation and suppresses IFN production. First, overexpression of P protein inhibited the IFN promoter activation induced by SVCV and the IFN activity activated by the mitochondrial antiviral signaling protein (MAVS) although TBK1 activity was not blocked by P protein. Second, P protein colocalized and interacted with TBK1. Dominant negative experiments suggested that the TBK1 N-terminal kinase domain interacted with P protein and was essential for P protein and IRF3 phosphorylation. Finally, P protein overexpression reduced the IRF3 phosphorylation activated by TBK1 and reduced host cellular ifn transcription. Collectively, our data demonstrated that the SVCV P protein is a decoy substrate for the host phosphokinase TBK1, preventing IFN production and facilitating SVCV replication. IMPORTANCE TBK1 is a pivotal phosphokinase that activates host IFN production to defend against viral infection; thus, it is a potential target for viruses to negatively regulate IFN response and facilitate viral evasion. We report that the SVCV P protein functions as a decoy substrate for cellular TBK1, leading to the reduction of IRF3 phosphorylation and suppression of IFN expression. These findings reveal a novel immune evasion mechanism of SVCV.

DOI10.1128/JVI.01381-16
Funding OrganizationNational Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS)
Indexed BySCI
Language英语
Funding ProjectNational Basic Research Program of China[2014CB138601] ; National Natural Science Foundation of China (NSFC)[31502200] ; Chinese Academy of Sciences (CAS)[Y65E01-1-501]
WOS Research AreaVirology
WOS SubjectVirology
WOS IDWOS:000389904500024
WOS KeywordANTIVIRAL SIGNALING PROTEIN ; VESICULAR STOMATITIS-VIRUS ; INNATE IMMUNE-RESPONSE ; I-LIKE RECEPTORS ; NF-KAPPA-B ; RIG-I ; TRANSCRIPTIONAL ACTIVITY ; VIRAL-INFECTION ; IFN RESPONSES ; IRF3
PublisherAMER SOC MICROBIOLOGY
Funding OrganizationNational Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS)
Citation statistics
Cited Times:21[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/31895
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorZhang, Yong-An
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Li, Shun,Lu, Long-Feng,Wang, Zhao-Xi,et al. The P Protein of Spring Viremia of Carp Virus Negatively Regulates the Fish Interferon Response by Inhibiting the Kinase Activity of TANK-Binding Kinase 1[J]. JOURNAL OF VIROLOGY,2016,90(23):10728-10737.
APA Li, Shun.,Lu, Long-Feng.,Wang, Zhao-Xi.,Lu, Xiao-Bing.,Chen, Dan-Dan.,...&Zhang, Yong-An.(2016).The P Protein of Spring Viremia of Carp Virus Negatively Regulates the Fish Interferon Response by Inhibiting the Kinase Activity of TANK-Binding Kinase 1.JOURNAL OF VIROLOGY,90(23),10728-10737.
MLA Li, Shun,et al."The P Protein of Spring Viremia of Carp Virus Negatively Regulates the Fish Interferon Response by Inhibiting the Kinase Activity of TANK-Binding Kinase 1".JOURNAL OF VIROLOGY 90.23(2016):10728-10737.
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