IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Nonsense-mediated mRNA decay in Tetrahymena is EJC independent and requires a protozoa-specific nuclease
Tian, Miao1,2,3; Yang, Wentao1,2; Zhang, Jing1,2; Dang, Huai4; Lu, Xingyi1,2; Fu, Chengjie1; Miao, Wei1
2017-06-20
Source PublicationNUCLEIC ACIDS RESEARCH
ISSN0305-1048
Volume45Issue:11Pages:6848-6863
Abstract

Nonsense-mediated mRNA decay (NMD) is essential for removing premature termination co-doncontaining transcripts from cells. Studying the NMD pathway in model organisms can help to elucidate the NMD mechanism in humans and improve our understanding of how this biologically important process has evolved. Ciliates are among the earliest branching eukaryotes; their NMD mechanism is poorly understood and may be primordial. We demonstrate that highly conserved Upf proteins (Upf1a, Upf2 and Upf3) are involved in the NMD pathway of the ciliate, Tetrahymena thermophila. We further show that a novel protozoa-specific nuclease, Smg6L, is responsible for destroying many NMD-targeted transcripts. Transcriptome-wide identification and characterization of NMD-targeted transcripts in vegetative Tetrahymena cells showed that many have exon-exon junctions downstream of the termination codon. However, Tetrahymena may lack a functional exon junction complex (EJC), and the Tetrahymena ortholog of an EJC core component, Mago nashi (Mag1), is dispensable for NMD. Therefore, NMD is EJC independent in this early branching eukaryote.

DOI10.1093/nar/gkx256
Funding OrganizationNatural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF)
Indexed BySCI
Language英语
Funding ProjectNatural Science Foundation of China[31525021] ; Natural Science Foundation of China[91631303] ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China[2013DFG32390] ; Austrian Science Fund (FWF)[P27313-B20]
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
WOS IDWOS:000403693000059
WOS KeywordEXON JUNCTION COMPLEX ; ENDONUCLEOLYTIC CLEAVAGE ; GENE-EXPRESSION ; NMD PATHWAY ; IN-VIVO ; DEGRADATION ; UPF1 ; THERMOPHILA ; DROSOPHILA ; BINDING
PublisherOXFORD UNIV PRESS
Funding OrganizationNatural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF) ; Austrian Science Fund (FWF)
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/31847
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorMiao, Wei
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China
3.Univ Vienna, Max F Perutz Labs, Dept Chromosome Biol, A-1030 Vienna, Austria
4.Northwest Normal Univ, Coll Life Sci, Lanzhou 730070, Peoples R China
Recommended Citation
GB/T 7714
Tian, Miao,Yang, Wentao,Zhang, Jing,et al. Nonsense-mediated mRNA decay in Tetrahymena is EJC independent and requires a protozoa-specific nuclease[J]. NUCLEIC ACIDS RESEARCH,2017,45(11):6848-6863.
APA Tian, Miao.,Yang, Wentao.,Zhang, Jing.,Dang, Huai.,Lu, Xingyi.,...&Miao, Wei.(2017).Nonsense-mediated mRNA decay in Tetrahymena is EJC independent and requires a protozoa-specific nuclease.NUCLEIC ACIDS RESEARCH,45(11),6848-6863.
MLA Tian, Miao,et al."Nonsense-mediated mRNA decay in Tetrahymena is EJC independent and requires a protozoa-specific nuclease".NUCLEIC ACIDS RESEARCH 45.11(2017):6848-6863.
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