IHB OpenIR  > 其他  > 期刊论文
Expression, Purification, and Characterization of Hemolytic Toxin from Virulent Aeromonas hydrophila
Dong, Jing1,2; Ruan, Jing3; Xu, Ning1,2; Yang, Yibin1,2; Ai, Xiaohui1,2
2017-06-01
Source PublicationJOURNAL OF THE WORLD AQUACULTURE SOCIETY
ISSN0893-8849
Volume48Issue:3Pages:531-536
Abstract

Aeromonas hydrophila is a major pathogen in aquaculture in China; it is also an emerging pathogen in humans and animals. In recent years, several severe diseases, such as gastroenteritis, meningitis, and skin and soft tissue infections, have been found to be caused by A. hydrophila. Aerolysin and hemolysin are the most important virulence factors secreted by A. hydrophila. Therefore, new drugs targeted at these two toxins can be found in the therapy for A. hydrophila infections, reducing the usage of antibiotics. In this paper, we expressed and purified the aerolysin and hemolysin using a prokaryotic expression system. We found that aerolysin was more toxic than hemolysin, both in vitro and in vivo. The lethal dose (LD50) of aerolysin and hemolysin was 0.4 and 2.3 mu g per fish, respectively.

DOI10.1111/jwas.12351
Funding OrganizationNational Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China
Indexed BySCI
Language英语
Funding ProjectNational Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS[2015JBFM37] ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS[2015JBFM29] ; Special Fund for Agro-Scientific Research in the Public Interest of China[201203085]
WOS Research AreaFisheries
WOS SubjectFisheries
WOS IDWOS:000402640300016
WOS KeywordAEROLYSIN ; WATER ; PROAEROLYSIN ; GENES ; MODEL
PublisherWILEY
Funding OrganizationNational Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/31807
Collection其他_期刊论文
Corresponding AuthorDong, Jing; Ai, Xiaohui
Affiliation1.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China
2.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Wuxi 214081, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Dong, Jing,Ruan, Jing,Xu, Ning,et al. Expression, Purification, and Characterization of Hemolytic Toxin from Virulent Aeromonas hydrophila[J]. JOURNAL OF THE WORLD AQUACULTURE SOCIETY,2017,48(3):531-536.
APA Dong, Jing,Ruan, Jing,Xu, Ning,Yang, Yibin,&Ai, Xiaohui.(2017).Expression, Purification, and Characterization of Hemolytic Toxin from Virulent Aeromonas hydrophila.JOURNAL OF THE WORLD AQUACULTURE SOCIETY,48(3),531-536.
MLA Dong, Jing,et al."Expression, Purification, and Characterization of Hemolytic Toxin from Virulent Aeromonas hydrophila".JOURNAL OF THE WORLD AQUACULTURE SOCIETY 48.3(2017):531-536.
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