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Differential gene content and gene expression for bacterial evolution and speciation of Shewanella in terms of biosynthesis of heme and heme-requiring proteins
Alternative TitleUniversity of Chinese Academy of Sciences, Beijing 100049, China
Dai,Jingcheng1,2; Liu,Yaqi1,2; Liu,Shuangyuan1,2; Li,Shuyang1,2; Gao,Na1,2; Wang,Jing1,2; Zhou,Jizhong3,4; Qiu,Dongru1,2
2019-07-30
Source PublicationBMC Microbiology
ISSN1471-2180
Volume19Issue:1Pages:1-19
Abstract

AbstractBackgroundMost species of Shewanella harbor two ferrochelatase paralogues for the biosynthesis of c-type cytochromes, which are crucial for their respiratory versatility. In our previous study of the Shewanella loihica PV-4 strain, we found that the disruption of hemH1 but not hemH2 resulted in a significant accumulation of extracellular protoporphyrin IX (PPIX), but it is different in Shewanella oneidensis MR-1. Hence, the function and transcriptional regulation of two ferrochelatase genes, hemH1 and hemH2, are investigated in S. oneidensis MR-1.ResultIn the present study, deletion of either hemH1 or hemH2 in S. oneidensis MR-1 did not lead to overproduction of extracellular protoporphyrin IX (PPIX) as previously described in the hemH1 mutants of S. loihica PV-4. Moreover, supplement of exogenous hemins made it possible to generate the hemH1 and hemH2 double mutant in MR-1, but not in PV-4. Under aerobic condition, exogenous hemins were required for the growth of MR-1ΔhemH1ΔhemH2, which also overproduced extracellular PPIX. These results suggest that heme is essential for aerobic growth of Shewanella species and MR-1 could also uptake hemin for biosynthesis of essential cytochrome(s) and respiration. Besides, the exogenous hemin mediated CymA cytochrome maturation and the cellular KatB catalase activity. Both hemH paralogues were transcribed in wild-type MR-1, and the hemH2 transcription was remarkably up-regulated in MR-1ΔhemH1 mutant to compensate for the loss of hemH1. The periplasmic glutathione peroxidase gene pgpD, located in the same operon with hemH2, and a large gene cluster coding for iron, heme (hemin) uptake systems are absent in the PV-4 genome.ConclusionOur results indicate that the genetic divergence in gene content and gene expression between these Shewanella species, accounting for the phenotypic difference described here, might be due to their speciation and adaptation to the specific habitats (iron-rich deep-sea vent versus iron-poor freshwater) in which they evolved and the generated mutants could potentially be utilized for commercial production of PPIX.

KeywordFerrochelatase Shewanella Protoporphyrin IX Cytochrome Hemin
DOI10.1186/s12866-019-1549-9
Language英语
WOS IDBMC:10.1186/s12866-019-1549-9
PublisherBioMed Central
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/31748
Collection水环境工程研究中心_期刊论文
Corresponding AuthorQiu,Dongru
Affiliation1.1Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, Hubei Province, China
2.University of Chinese Academy of Sciences, Beijing 100049, China
3.Institute for Environmental Genomics, and Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK 73019, USA.
4.Earth Science Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94270, USA.
Recommended Citation
GB/T 7714
Dai,Jingcheng,Liu,Yaqi,Liu,Shuangyuan,et al. Differential gene content and gene expression for bacterial evolution and speciation of Shewanella in terms of biosynthesis of heme and heme-requiring proteins[J]. BMC Microbiology,2019,19(1):1-19.
APA Dai,Jingcheng.,Liu,Yaqi.,Liu,Shuangyuan.,Li,Shuyang.,Gao,Na.,...&Qiu,Dongru.(2019).Differential gene content and gene expression for bacterial evolution and speciation of Shewanella in terms of biosynthesis of heme and heme-requiring proteins.BMC Microbiology,19(1),1-19.
MLA Dai,Jingcheng,et al."Differential gene content and gene expression for bacterial evolution and speciation of Shewanella in terms of biosynthesis of heme and heme-requiring proteins".BMC Microbiology 19.1(2019):1-19.
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