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Comparative study of the immunoprotective effect of two grass carp-sourced Bacillus subtilis spore-based vaccines against grass carp reovirus
Chen, Dan-Dan1; Yao, Yuan-Yuan1,3; Cui, Zheng-Wei1,3; Zhang, Xiang-Yang1,3; Guo, Xia4; Zhou, Yuan-Yuan1,3; Zhang, Yong-An1,2
2019-04-15
Source PublicationAQUACULTURE
ISSN0044-8486
Volume504Issue:1Pages:88-95
Abstract

Grass carp reovirus II (GCRV II) causes severe haemorrhagic disease with high mortality in grass carp, Cyenopharyngodon idellus. Vaccination is a very effective method in conferring protection against GCRV. In the current study, we constructed two grass carp-sourced Bacillus subtilis spore-based vaccines (GC5-VP4 and GC5NS38) and investigated their potential as oral candidate vaccines against GCRV II. Our results showed that both VP4 and NS38 proteins of GCRV II were efficiently displayed on the spore surface of B. subtilis GC5, and the two recombinant spores stayed in the hindguts of grass carp for antigen presentation. After oral administration, compared with the control groups, both recombinant vaccines GC5-VP4 and GC5-NS38 increased the survival rate of grass carp against GCRV II, with relative percent survival rates of 30% and 36.4%, respectively. Although they had a similar immunoprotective effect, the mechanisms were very different. GC5-VP4 mainly elicited specific antibody responses, both systemic and mucosal, whereas GC5-NS38 mainly elicited an inflammatory response and cellular immunity via expression of major histocompatibility complex molecules. Furthermore, this study demonstrated that the recombinant spores triggered high levels of mucosal immunity and would be promising oral candidate vaccines against GCRV II in grass carp. Combined with previous research, we inferred that vaccine efficacy was not only related to antigen, but also vaccination strategies.

KeywordGrass carp reovirus Bacillus subtilis spore Oral vaccine Immunoprotection Immune response
DOI10.1016/j.aquaculture.2019.01.055
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; 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Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31725026] ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China[2018CFA011]
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000459962400011
WOS KeywordCOMPLETE GENOME SEQUENCE ; RAINBOW-TROUT ; CLONORCHIS-SINENSIS ; IMMUNE-RESPONSES ; SUBUNIT VACCINE ; GENE-EXPRESSION ; ORAL DELIVERY ; CELL ; FISH ; ANTIGEN
PublisherELSEVIER SCIENCE BV
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; 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Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/31643
Collection其他_期刊论文
Corresponding AuthorZhang, Yong-An
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Hubei, Peoples R China
2.Huazhong Agr Univ, Coll Fisheries, State Key Lab Agr Microbiol, Wuhan, Hubei, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Chen, Dan-Dan,Yao, Yuan-Yuan,Cui, Zheng-Wei,et al. Comparative study of the immunoprotective effect of two grass carp-sourced Bacillus subtilis spore-based vaccines against grass carp reovirus[J]. AQUACULTURE,2019,504(1):88-95.
APA Chen, Dan-Dan.,Yao, Yuan-Yuan.,Cui, Zheng-Wei.,Zhang, Xiang-Yang.,Guo, Xia.,...&Zhang, Yong-An.(2019).Comparative study of the immunoprotective effect of two grass carp-sourced Bacillus subtilis spore-based vaccines against grass carp reovirus.AQUACULTURE,504(1),88-95.
MLA Chen, Dan-Dan,et al."Comparative study of the immunoprotective effect of two grass carp-sourced Bacillus subtilis spore-based vaccines against grass carp reovirus".AQUACULTURE 504.1(2019):88-95.
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