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Molecular characterization and expression analysis of four fish-specific CC chemokine receptors CCR4La, CCR4Lc1, CCR4Lc2 and CCR11 in rainbow trout (Oncorhynchus mykiss)
Qi, Zhitao1,2,5; Holland, Jason W.1; Jiang, Yousheng1,3; Secombes, Christopher J.1; Nie, Pin4; Wang, Tiehui1
2017-09-01
Conference Name12th International Symposium on Genetics in Aquaculture
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume68
Issue1
Pages411-427
Conference DateJUN 21-27, 2015
Conference PlaceSantiago de Compostela, SPAIN
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
ISSN1050-4648
Abstract

The chemokine and chemokine receptor networks regulate leukocyte trafficking, inflammation, immune cell differentiation, cancer and other biological processes. Comparative immunological studies have revealed that both chemokines and their receptors have expanded greatly in a species/lineage specific way. Of the 10 human CC chemokine receptors (CCR1-10) that bind CC chemokines, orthologues only to CCR6, 7, 9 and 10 are present in teleost fish. In this study, four fish-specific CCRs, termed as CCR4La, CCR4Lc1, CCR4Lc2 and CCR11, with a close link to human CCR1-5 and 8, in terms of amino acid homology and syntenic conservation, have been identified and characterized in rainbow trout (Oncorhynchus mykiss). These CCRs were found to possess the conserved features of the G protein-linked receptor family, including an extracellular N-terminal, seven TM domains, three extracellular loops and three intracellular loops, and a cytoplasmic carboxyl tail with multiple potential serine/threonine phosphorylation sites. Four cysteine residues known to be involved in forming two disulfide bonds are present in the extracellular domains and a DRY motif is present in the second intracellular loop. Signaling mediated by these receptors might be regulated by N-glycosylation, tyrosine sulfation, S-palmitoylation, a PDZ ligand motif and di-leucine motifs. Studies of intron/exon structure revealed distinct fish-specific CCR gene organization in different fish species/lineages that might contribute to the diversification of the chemokine ligand-receptor networks in different fish lineages. Fish-specific trout CCRs are highly expressed in immune tissues/organs, such as thymus, spleen, head kidney and gills. Their expression can be induced by the pro-inflammatory cytokines, IL-1 beta,IL-6 and IFN gamma, by the pathogen associated molecular patterns, PolylC and peptidoglycan, and by bacterial infection. These data suggest that fish specific CCRs are likely to have an important role in immune regulation in fish. (C) 2017 Elsevier Ltd. All rights reserved.

KeywordRainbow trout CC chemokine receptor CCR4La CCR4Lc CCR11 Expression Modulation Pro-inflammatory cytokine Bacterial infection Parasitic infection
Funding OrganizationQinglan project of Jiangsu Province ; Qinglan project of Jiangsu Province ; Qinglan project of Jiangsu Province ; Qinglan project of Jiangsu Province ; Overseas Training Plan for Young and Middle-aged Teachers and Principals of College and Universities in Jiangsu Province, China ; Overseas Training Plan for Young and Middle-aged Teachers and Principals of College and Universities in Jiangsu Province, China ; Overseas Training Plan for Young and Middle-aged Teachers and Principals of College and Universities in Jiangsu Province, China ; Overseas Training Plan for Young and Middle-aged Teachers and Principals of College and Universities in Jiangsu Province, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Jiangsu Province ; Jiangsu Province ; Jiangsu Province ; Jiangsu Province ; Marine Alliance for Science and Technology for Scotland (MASTS), a pooling initiative ; Marine Alliance for Science and Technology for Scotland (MASTS), a pooling initiative ; Marine Alliance for Science and Technology for Scotland (MASTS), a pooling initiative ; Marine Alliance for Science and Technology for Scotland (MASTS), a pooling initiative ; Scottish Funding Council ; Scottish Funding Council ; Scottish Funding Council ; Scottish Funding Council ; Swiss National Science Foundation ; Swiss National Science Foundation ; Swiss National Science Foundation ; Swiss National Science Foundation ; Qinglan project of Jiangsu Province ; Qinglan project of Jiangsu Province ; Qinglan project of Jiangsu Province ; Qinglan project of Jiangsu Province ; Overseas Training Plan for Young and Middle-aged Teachers and Principals of College and Universities in Jiangsu Province, China ; Overseas Training Plan for Young and Middle-aged Teachers and Principals of College and Universities in Jiangsu Province, China ; Overseas Training Plan for Young and Middle-aged Teachers and Principals of College and Universities in Jiangsu Province, China ; Overseas Training Plan for Young and Middle-aged Teachers and Principals of College and Universities in Jiangsu Province, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Jiangsu Province ; Jiangsu Province ; Jiangsu Province ; Jiangsu Province ; Marine Alliance for Science and Technology for Scotland (MASTS), a pooling initiative ; Marine Alliance for Science and Technology for Scotland (MASTS), a pooling initiative ; Marine Alliance for Science and Technology for Scotland (MASTS), a pooling initiative ; Marine Alliance for Science and Technology for Scotland (MASTS), a pooling initiative ; Scottish Funding Council ; Scottish Funding Council ; Scottish Funding Council ; Scottish Funding Council ; Swiss National Science Foundation ; Swiss National Science Foundation ; Swiss National Science Foundation ; Swiss National Science Foundation ; Qinglan project of Jiangsu Province ; Qinglan project of Jiangsu Province ; Qinglan project of Jiangsu Province ; Qinglan project of Jiangsu Province ; Overseas Training Plan for Young and Middle-aged Teachers and Principals of College and Universities in Jiangsu Province, China ; Overseas Training Plan for Young and Middle-aged Teachers and Principals of College and Universities in Jiangsu Province, China ; Overseas Training Plan for Young and Middle-aged Teachers and Principals of College and Universities in Jiangsu Province, China ; Overseas Training Plan for Young and Middle-aged Teachers and Principals of College and Universities in Jiangsu Province, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Jiangsu Province ; Jiangsu Province ; Jiangsu Province ; Jiangsu Province ; Marine Alliance for Science and Technology for Scotland (MASTS), a pooling initiative ; Marine Alliance for Science and Technology for Scotland (MASTS), a pooling initiative ; Marine Alliance for Science and Technology for Scotland (MASTS), a pooling initiative ; Marine Alliance for Science and Technology for Scotland (MASTS), a pooling initiative ; Scottish Funding Council ; Scottish Funding Council ; Scottish Funding Council ; Scottish Funding Council ; Swiss National Science Foundation ; Swiss National Science Foundation ; Swiss National Science Foundation ; Swiss National Science Foundation ; Qinglan project of Jiangsu Province ; Qinglan project of Jiangsu Province ; Qinglan project of Jiangsu Province ; Qinglan project of Jiangsu Province ; Overseas Training Plan for Young and Middle-aged Teachers and Principals of College and Universities in Jiangsu Province, China ; Overseas Training Plan for Young and Middle-aged Teachers and Principals of College and Universities in Jiangsu Province, China ; Overseas Training Plan for Young and Middle-aged Teachers and Principals of College and Universities in Jiangsu Province, China ; Overseas Training Plan for Young and Middle-aged Teachers and Principals of College and Universities in Jiangsu Province, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Jiangsu Province ; Jiangsu Province ; Jiangsu Province ; Jiangsu Province ; Marine Alliance for Science and Technology for Scotland (MASTS), a pooling initiative ; Marine Alliance for Science and Technology for Scotland (MASTS), a pooling initiative ; Marine Alliance for Science and Technology for Scotland (MASTS), a pooling initiative ; Marine Alliance for Science and Technology for Scotland (MASTS), a pooling initiative ; Scottish Funding Council ; Scottish Funding Council ; Scottish Funding Council ; Scottish Funding Council ; Swiss National Science Foundation ; Swiss National Science Foundation ; Swiss National Science Foundation ; Swiss National Science Foundation
DOI10.1016/j.fsi.2017.07.031
Indexed BySCI
Funding ProjectQinglan project of Jiangsu Province ; Overseas Training Plan for Young and Middle-aged Teachers and Principals of College and Universities in Jiangsu Province, China ; National Natural Science Foundation of China[31302221] ; National Natural Science Foundation of China[31272666] ; Jiangsu Province[BK2011418] ; Jiangsu Province[BK20151297] ; Marine Alliance for Science and Technology for Scotland (MASTS), a pooling initiative ; Scottish Funding Council[HR09011] ; Swiss National Science Foundation[CRSII3_147649-1]
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000411299500045
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type会议论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/31609
Collection鱼类生物学及渔业生物技术研究中心_会议论文
Corresponding AuthorWang, Tiehui
Affiliation1.Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Aberdeen AB24 2TZ, Scotland
2.Yancheng Inst Technol, Key Lab Biochem & Biotechnol Marine Wetland Jiang, Yancheng 224051, Jiangsu, Peoples R China
3.Shanghai Ocean Univ, Coll Fishery & Life Sci, Shanghai 201306, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
5.Yangtze Univ, Coll Anim Sci, Jingzhou 434020, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Qi, Zhitao,Holland, Jason W.,Jiang, Yousheng,et al. Molecular characterization and expression analysis of four fish-specific CC chemokine receptors CCR4La, CCR4Lc1, CCR4Lc2 and CCR11 in rainbow trout (Oncorhynchus mykiss)[C]:ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD,2017:411-427.
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