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The first report of diablo in Megalobrama amblycephala: characterization, phylogenetic analysis, functional annotation and expression
Ngoc Tuan Tran1,2,3; Jakovlic, Ivan1; Wang, Wei-Min1,4
Corresponding AuthorWang, Wei-Min(wangwm@mail.hzau.edu.cn)
2017-09-01
Source PublicationJOURNAL OF GENETICS
ISSN0022-1333
Volume96Issue:4Pages:613-623
AbstractSmac/DIABLO gene is essential for the apoptosis mechanism in mammals. This study is the first report of the Megalobrama amblycephala (ma) diablo gene, and the first report of the tertiary structure of a Diablo polypeptide in fish. Madiablo is 1540-bp long with an open reading frame of 792 bp, encoding a putative protein of 263 amino acids with a molecular weight of 29.2 kDa. Phylogenetic analysis indicates that it is closely related to the zebrafish Diablo-a homologue. It also indicates the existence of two diablo copies (a and b) in teleosts; apart from the Percomorpha group, where diablo-b has been lost, but diablo-a had undergone an independent duplication. Madiablo protein contains a long Smac_DIABLO super family domain (Leu32-Asp263) and alpha helices were prevalent in the secondary structure. Homology model of madiablo protein was constructed using the comparative modelling method. Expression of madiablo mRNA transcript was investigated using qPCR: (i) in five tissues from a healthy blunt snout bream, indicating the highest constitutive expression level in liver. (ii) During the embryo and juvenile development, indicating a spike in expression during hatching and in later phases of the juvenile development. (iii) In response to Aeromonas hydrophila infection, indicating the downregulation in liver, spleen and kidney during the first 12 h postinfection and upregulation in spleen and kidney after 24 h postinfection (hpi). The results imply that madiablo is homologous to Diablo orthologues in other species, both structurally and functionally, and that, it probably plays a role in the immune system of M. amblycephala.
KeywordDiablo physicochemical characterization homology modelling functional annotation gene expression Megalobrama amblycephala
DOI10.1007/s12041-017-0816-5
Funding OrganizationChina Scholarship Council ; China Scholarship Council ; China Scholarship Council ; China Scholarship Council ; China Scholarship Council ; China Scholarship Council ; China Scholarship Council ; China Scholarship Council ; China Scholarship Council ; China Scholarship Council ; China Scholarship Council ; China Scholarship Council ; China Scholarship Council ; China Scholarship Council ; China Scholarship Council ; China Scholarship Council
Indexed BySCI ; SCI
Language英语
Funding ProjectChina Scholarship Council
WOS Research AreaGenetics & Heredity
WOS SubjectGenetics & Heredity
WOS IDWOS:000412537100009
WOS KeywordSECONDARY STRUCTURE PREDICTION ; AEROMONAS-HYDROPHILA ; GENOME DUPLICATION ; IAP PROTEINS ; TELEOST FISH ; APOPTOSIS ; SMAC/DIABLO ; GENES ; ACTIVATION ; MECHANISMS
PublisherINDIAN ACAD SCIENCES
Funding OrganizationChina Scholarship Council ; China Scholarship Council ; China Scholarship Council ; China Scholarship Council ; China Scholarship Council ; China Scholarship Council ; China Scholarship Council ; China Scholarship Council ; China Scholarship Council ; China Scholarship Council ; China Scholarship Council ; China Scholarship Council ; China Scholarship Council ; China Scholarship Council ; China Scholarship Council ; China Scholarship Council
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/31600
Collection其他_期刊论文
Corresponding AuthorWang, Wei-Min
Affiliation1.Huazhong Agr Univ, Key Lab Freshwater Anim Breeding, Key Lab Agr Anim Genet Breeding & Reprod, Minist Educ,Minist Agr,Coll Fisheries, Wuhan 430070, Hubei, Peoples R China
2.Chinese Acad Sci, Minist Agr, Key Lab Aquaculture Dis Control, Wuhan 430072, Hubei, Peoples R China
3.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
4.Collaborat Innovat Ctr Efficient & Hlth Prod Fish, Changde 41500, Hunan, Peoples R China
Recommended Citation
GB/T 7714
Ngoc Tuan Tran,Jakovlic, Ivan,Wang, Wei-Min. The first report of diablo in Megalobrama amblycephala: characterization, phylogenetic analysis, functional annotation and expression[J]. JOURNAL OF GENETICS,2017,96(4):613-623.
APA Ngoc Tuan Tran,Jakovlic, Ivan,&Wang, Wei-Min.(2017).The first report of diablo in Megalobrama amblycephala: characterization, phylogenetic analysis, functional annotation and expression.JOURNAL OF GENETICS,96(4),613-623.
MLA Ngoc Tuan Tran,et al."The first report of diablo in Megalobrama amblycephala: characterization, phylogenetic analysis, functional annotation and expression".JOURNAL OF GENETICS 96.4(2017):613-623.
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