IHB OpenIR  > 淡水生态学研究中心  > 期刊论文
Computational identification of Y-linked markers and genes in the grass carp genome by using a pool-and-sequence method
Zhang, Aidi1; Huang, Rong1; Chen, Liangming1,2; Xiong, Lv1,2; He, Libo1; Li, Yongming1; Liao, Lanjie1; Zhu, Zuoyan1; Wang, Yaping1
2017-08-15
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
Volume7Issue:1Pages:12
Abstract

The molecular analysis of sex in vertebrates is important, as it has the potential to provide vital information for theoretical and applied research alike. Teleost fish are the ancient vertebrates that present a broad sex chromosome system but lack differentiated sex chromosomes in most species. Hence understanding the sex in fish would not only illuminate the sex determination evolution in vertebrates but also shed light on fish farming. In the present study, we used grass carp as a teleost fish model, studied the Y chromosome by using a pool-and-sequence strategy in combination with fragment-ratio method. In total, we identified five Y-linked scaffolds (totaling 347 Kb) and six Y-specific sequences that could be used as sex-specific markers, demonstrating the suitability of NGS-based re-sequencing of pooled DNAs for the identification of sex markers in fish. Moreover, 14 putative Y-linked genes were described for the first time. All the genes, except for un-y1, un-y2, and ubq-y, showed high similarity to their female homologs. RT-PCR revealed that ubq-y was only expressed in the male hypothalamus and pituitary. These findings provided an abundant resource for the Y chromosome of grass carp, and may help elucidate sex chromosome evolution in cyprinid fish.

DOI10.1038/s41598-017-08476-y
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31130055] ; Strategic Pilot Science and Technology Projects (A) Category ; Chinese Academy of Science[XDA08030203]
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000407570000090
WOS KeywordBULKED SEGREGANT ANALYSIS ; SEX-CHROMOSOME SYSTEMS ; CTENOPHARYNGODON-IDELLUS ; RAD-SEQ ; FISH ; EVOLUTION ; PROTEIN ; DIFFERENTIATION ; RESISTANCE ; DIVERSITY
PublisherNATURE PUBLISHING GROUP
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Strategic Pilot Science and Technology Projects (A) Category ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/31482
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorWang, Yaping
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Aidi,Huang, Rong,Chen, Liangming,et al. Computational identification of Y-linked markers and genes in the grass carp genome by using a pool-and-sequence method[J]. SCIENTIFIC REPORTS,2017,7(1):12.
APA Zhang, Aidi.,Huang, Rong.,Chen, Liangming.,Xiong, Lv.,He, Libo.,...&Wang, Yaping.(2017).Computational identification of Y-linked markers and genes in the grass carp genome by using a pool-and-sequence method.SCIENTIFIC REPORTS,7(1),12.
MLA Zhang, Aidi,et al."Computational identification of Y-linked markers and genes in the grass carp genome by using a pool-and-sequence method".SCIENTIFIC REPORTS 7.1(2017):12.
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