| Heterologous expression of mlrA gene originated from Novosphingobium sp THN1 to degrade microcystin-RR and identify the first step involved in degradation pathway | |
| Wang, Ruiping1; Li, Jieming1; Jiang, Yongguang2; Lu, Zhijiang3; Li, Renhui4; Li, Ji1 | |
| Corresponding Author | Li, Ji(lijieming@cau.edu.cn) |
| 2017-10-01 | |
| Source Publication | CHEMOSPHERE
 |
| ISSN | 0045-6535 |
| Volume | 184Pages:159-167 |
| Abstract | Information on the catalytic role of mlrA gene-encoded enzyme (MlrA) in microcystin-RR (MC-RR) biodegradation was limited. This study succeeded in expressing mlrA homolog of Novosphingobium sp. THN1 in heterologous host for the first time, by constructing a recombinant bacterium. Mass spectrometric analysis showed that the recombinant MlrA hydrolyzed MC-RR into linear intermediate product by cleaving the peptide bond between Adda and arginine residue, greatly detoxifying MC-RR. This finding clearly manifested that the MlrA homolog of THN1 strain possesses its original catalytic function, and ring-opening constituted the first step in MC-RR biodegradation pathway of THN1 strain. Moreover, MC-RR degradation by intact recombinant cells and cell-free crude enzyme (CE) from recombinant was compared. Results exhibited that intact recombinant was able to degrade 20 mu g mL(-1) MC-RR more quickly than CE, with the maximum rate of 9.22 mu g mL(-1) h(-1) in the first 8 h. Thus, this study provided new insights on the catalytic activity and roles of MIrA originated from THN1 strain in MC-RR biodegradation process, which lay a foundation for efficiently removing and detoxifying MC-RR, and exploring downstream steps in MC-RR biodegradation pathway of THN1 strain. (C) 2017 Elsevier Ltd. All rights reserved. |
| Keyword | Biodegradation Heterologous expression Microcystin-RR mlrA gene Novosphingobium sp THN1 |
| DOI | 10.1016/j.chemosphere.2017.05.086 |
| Funding Organization | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China |
| Indexed By | SCI ; SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[31300434] ; Research Fund for the Doctoral Program of Higher Education of China[20130008120026] |
| WOS Research Area | Environmental Sciences & Ecology |
| WOS Subject | Environmental Sciences |
| WOS ID | WOS:000407525500019 |
| WOS Keyword | BIOLOGICAL TREATMENT FACILITY ; BACTERIAL-DEGRADATION ; NUTRIENT CONDITIONS ; ENZYMATIC PATHWAY ; PICHIA-PASTORIS ; BIODEGRADATION ; LR ; CLONING ; HEPATOTOXINS ; CYANOBACTERIA |
| Publisher | PERGAMON-ELSEVIER SCIENCE LTD |
| Funding Organization | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/31473 |
| Collection | 藻类生物学及应用研究中心_期刊论文 |
| Corresponding Author | Li, Ji |
| Affiliation | 1.China Agr Univ, Coll Resources & Environm Sci, Beijing 100193, Peoples R China 2.Shenzhen Univ, Shenzhen Key Lab Marine Bioresource & Ecoenvironm, Shenzhen 518060, Guangdong, Peoples R China 3.China Inst Water Resources & Hydropower Res, Dept Irrigat & Drainage, Beijing 100048, Peoples R China 4.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China |
| Recommended Citation GB/T 7714 | Wang, Ruiping,Li, Jieming,Jiang, Yongguang,et al. Heterologous expression of mlrA gene originated from Novosphingobium sp THN1 to degrade microcystin-RR and identify the first step involved in degradation pathway[J]. CHEMOSPHERE,2017,184:159-167. |
| APA | Wang, Ruiping,Li, Jieming,Jiang, Yongguang,Lu, Zhijiang,Li, Renhui,&Li, Ji.(2017).Heterologous expression of mlrA gene originated from Novosphingobium sp THN1 to degrade microcystin-RR and identify the first step involved in degradation pathway.CHEMOSPHERE,184,159-167. |
| MLA | Wang, Ruiping,et al."Heterologous expression of mlrA gene originated from Novosphingobium sp THN1 to degrade microcystin-RR and identify the first step involved in degradation pathway".CHEMOSPHERE 184(2017):159-167. |
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