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Heterologous expression of mlrA gene originated from Novosphingobium sp THN1 to degrade microcystin-RR and identify the first step involved in degradation pathway
Wang, Ruiping1; Li, Jieming1; Jiang, Yongguang2; Lu, Zhijiang3; Li, Renhui4; Li, Ji1
2017-10-01
Source PublicationCHEMOSPHERE
ISSN0045-6535
Volume184Issue:1Pages:159-167
Abstract

Information on the catalytic role of mlrA gene-encoded enzyme (MlrA) in microcystin-RR (MC-RR) biodegradation was limited. This study succeeded in expressing mlrA homolog of Novosphingobium sp. THN1 in heterologous host for the first time, by constructing a recombinant bacterium. Mass spectrometric analysis showed that the recombinant MlrA hydrolyzed MC-RR into linear intermediate product by cleaving the peptide bond between Adda and arginine residue, greatly detoxifying MC-RR. This finding clearly manifested that the MlrA homolog of THN1 strain possesses its original catalytic function, and ring-opening constituted the first step in MC-RR biodegradation pathway of THN1 strain. Moreover, MC-RR degradation by intact recombinant cells and cell-free crude enzyme (CE) from recombinant was compared. Results exhibited that intact recombinant was able to degrade 20 mu g mL(-1) MC-RR more quickly than CE, with the maximum rate of 9.22 mu g mL(-1) h(-1) in the first 8 h. Thus, this study provided new insights on the catalytic activity and roles of MIrA originated from THN1 strain in MC-RR biodegradation process, which lay a foundation for efficiently removing and detoxifying MC-RR, and exploring downstream steps in MC-RR biodegradation pathway of THN1 strain. (C) 2017 Elsevier Ltd. All rights reserved.

KeywordBiodegradation Heterologous expression Microcystin-RR mlrA gene Novosphingobium sp THN1
DOI10.1016/j.chemosphere.2017.05.086
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31300434] ; Research Fund for the Doctoral Program of Higher Education of China[20130008120026]
WOS Research AreaEnvironmental Sciences & Ecology
WOS SubjectEnvironmental Sciences
WOS IDWOS:000407525500019
WOS KeywordBIOLOGICAL TREATMENT FACILITY ; BACTERIAL-DEGRADATION ; NUTRIENT CONDITIONS ; ENZYMATIC PATHWAY ; PICHIA-PASTORIS ; BIODEGRADATION ; LR ; CLONING ; HEPATOTOXINS ; CYANOBACTERIA
PublisherPERGAMON-ELSEVIER SCIENCE LTD
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China ; Research Fund for the Doctoral Program of Higher Education of China
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/31473
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorLi, Ji
Affiliation1.China Agr Univ, Coll Resources & Environm Sci, Beijing 100193, Peoples R China
2.Shenzhen Univ, Shenzhen Key Lab Marine Bioresource & Ecoenvironm, Shenzhen 518060, Guangdong, Peoples R China
3.China Inst Water Resources & Hydropower Res, Dept Irrigat & Drainage, Beijing 100048, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Wang, Ruiping,Li, Jieming,Jiang, Yongguang,et al. Heterologous expression of mlrA gene originated from Novosphingobium sp THN1 to degrade microcystin-RR and identify the first step involved in degradation pathway[J]. CHEMOSPHERE,2017,184(1):159-167.
APA Wang, Ruiping,Li, Jieming,Jiang, Yongguang,Lu, Zhijiang,Li, Renhui,&Li, Ji.(2017).Heterologous expression of mlrA gene originated from Novosphingobium sp THN1 to degrade microcystin-RR and identify the first step involved in degradation pathway.CHEMOSPHERE,184(1),159-167.
MLA Wang, Ruiping,et al."Heterologous expression of mlrA gene originated from Novosphingobium sp THN1 to degrade microcystin-RR and identify the first step involved in degradation pathway".CHEMOSPHERE 184.1(2017):159-167.
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