| DEVELOPMENT OF EST-DERIVED MICROSATELLITE MARKERS IN THE AQUATIC MACROPHYTE RANUNCULUS BUNGEI (RANUNCULACEAE) | |
Wu, Zhigang1,2; Wu, Jinwei3; Wang, Yalin2; Hou, Hongwei1,2
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| Corresponding Author | Hou, Hongwei(houhw@ihb.ac.cn) |
| 2017-07-01 | |
| Source Publication | APPLICATIONS IN PLANT SCIENCES
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| ISSN | 2168-0450 |
| Volume | 5Issue:7Pages:4 |
| Abstract | Premise of the study: Microsatellite or simple sequence repeat (SSR) markers were developed to investigate the influence of ecological factors on gene flow and spatial genetic structuring of the submerged plant Ranunculus bungei (Ranunculaceae), which is regarded as an important species for understanding how plants adapt to an aquatic environment. Methods and Results: Twenty-twomicrosatellite loci were identified from an expressed sequence tag (EST) library. The number of alleles per locus ranged from one to five, and the expected heterozygosity varied from 0.0 to 0.5 in four Chinese populations of R. bungei. Fourteen loci were polymorphic and significantly deviated from Hardy-Weinberg equilibrium. All of the loci were found to be amplifiable in two other species of Ranunculus section Batrachium, and cross-amplification in six riparian and aquatic species of Ranunculaceae was also partially successful. Conclusions: These novel EST-SSRmarkers will be useful for ecological and evolutionary studies of R. bungei as well as related species. |
| Keyword | aquatic plant genetic diversity microsatellite Ranunculaceae Ranunculus bungei |
| DOI | 10.3732/apps.1700022 |
| Funding Organization | State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology |
| Indexed By | SCI ; SCI |
| Language | 英语 |
| Funding Project | State Key Laboratory of Freshwater Ecology and Biotechnology[2016FB04] |
| WOS Research Area | Plant Sciences |
| WOS Subject | Plant Sciences |
| WOS ID | WOS:000407198300004 |
| WOS Keyword | SOFTWARE ; L. |
| Publisher | BOTANICAL SOC AMER INC |
| Funding Organization | State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/31451 |
| Collection | 水生生物分子与细胞生物学研究中心_期刊论文 |
| Corresponding Author | Hou, Hongwei |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 3.Wuhan Univ, Coll Life Sci, Dept Ecol, Wuhan 430072, Hubei, Peoples R China |
| Recommended Citation GB/T 7714 | Wu, Zhigang,Wu, Jinwei,Wang, Yalin,et al. DEVELOPMENT OF EST-DERIVED MICROSATELLITE MARKERS IN THE AQUATIC MACROPHYTE RANUNCULUS BUNGEI (RANUNCULACEAE)[J]. APPLICATIONS IN PLANT SCIENCES,2017,5(7):4. |
| APA | Wu, Zhigang,Wu, Jinwei,Wang, Yalin,&Hou, Hongwei.(2017).DEVELOPMENT OF EST-DERIVED MICROSATELLITE MARKERS IN THE AQUATIC MACROPHYTE RANUNCULUS BUNGEI (RANUNCULACEAE).APPLICATIONS IN PLANT SCIENCES,5(7),4. |
| MLA | Wu, Zhigang,et al."DEVELOPMENT OF EST-DERIVED MICROSATELLITE MARKERS IN THE AQUATIC MACROPHYTE RANUNCULUS BUNGEI (RANUNCULACEAE)".APPLICATIONS IN PLANT SCIENCES 5.7(2017):4. |
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