| Cloning, expression pattern and promoter functional analysis of cyp19a1a gene in miiuy croaker | |
| Huang, Wei1,2; Yang, Pan1; Lv, Zhenming1; Wu, Changwen1; Gui, Jianfang3; Lou, Bao2 | |
| Corresponding Author | Huang, Wei(huangwei@zjou.edu.cn) ; Lou, Bao(kfcsoa2017@163.com) |
| 2017-09-05 | |
| Source Publication | GENE
 |
| ISSN | 0378-1119 |
| Volume | 627Pages:271-277 |
| Abstract | Gonadal-specific aromatase encoded by cyp19a1a is the important enzyme controlling estrogen biosynthesis in teleosts. In the present study, the cDNA sequence of cyp19a1a was cloned and characterized from miiuy croaker Miichthys miiuy. The cDNA encoded a protein of 519 amino acids with five structural regions. Higher identities of amino acid sequences and conserved structural regions were found between Mmcyp19a1a and other cyp19a1a genes. In addition, Mmcyp19a1a was clustered together with other seawater fishes. Immunohistochemical analysis revealed that Mmcyp19a1a was localized exclusively in the cytoplasmic of thecal and granulosa cells surrounding the oocytes. Both the protein and mRNA levels of Mmcyp19a1a were increased significantly at the stage III follicles (mid-vitellogenic) and then decreased along with vitellogenesis. Interestingly, strong immunoreactive signals were also detected in the supporting cells of connective tissues during ovarian development. A 1777 bp promoter fragment of Mmcyp19a1a was also isolated, and functional analysis using an EGFP reporter fusion in zebrafish larvae presented positive signals in the above of yolk sac, where is the region of pronephros and germ plasm occur. The Mmcyp19a1a:EGFP expression pattern was generally consistent with the endogenous cyp19a1a genesis. These results indicate that the Mmcyp19a1a gene plays an important role during vitellogenesis and oocyte maturation. The constructor of Mmcyp19a1a:EGFP may provide a useful tool for genetic analysis of gonad development in teleost. |
| Keyword | cyp19a1a Vitellogenesis Promoter analysis Miichthys miiuy |
| DOI | 10.1016/j.gene.2017.06.042 |
| Funding Organization | Zhejiang Provincial Natural Science Foundation of China ; Zhejiang Provincial Natural Science Foundation of China ; Zhejiang Provincial Natural Science Foundation of China ; Zhejiang Provincial Natural Science Foundation of China ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Research Project of the Department of Education of Zhejiang Province ; Research Project of the Department of Education of Zhejiang Province ; Research Project of the Department of Education of Zhejiang Province ; Research Project of the Department of Education of Zhejiang Province ; Zhejiang Provincial Natural Science Foundation of China ; Zhejiang Provincial Natural Science Foundation of China ; Zhejiang Provincial Natural Science Foundation of China ; Zhejiang Provincial Natural Science Foundation of China ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Research Project of the Department of Education of Zhejiang Province ; Research Project of the Department of Education of Zhejiang Province ; Research Project of the Department of Education of Zhejiang Province ; Research Project of the Department of Education of Zhejiang Province ; Zhejiang Provincial Natural Science Foundation of China ; Zhejiang Provincial Natural Science Foundation of China ; Zhejiang Provincial Natural Science Foundation of China ; Zhejiang Provincial Natural Science Foundation of China ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Research Project of the Department of Education of Zhejiang Province ; Research Project of the Department of Education of Zhejiang Province ; Research Project of the Department of Education of Zhejiang Province ; Research Project of the Department of Education of Zhejiang Province ; Zhejiang Provincial Natural Science Foundation of China ; Zhejiang Provincial Natural Science Foundation of China ; Zhejiang Provincial Natural Science Foundation of China ; Zhejiang Provincial Natural Science Foundation of China ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Research Project of the Department of Education of Zhejiang Province ; Research Project of the Department of Education of Zhejiang Province ; Research Project of the Department of Education of Zhejiang Province ; Research Project of the Department of Education of Zhejiang Province |
| Indexed By | SCI ; SCI |
| Language | 英语 |
| Funding Project | Zhejiang Provincial Natural Science Foundation of China[LY14C190003] ; Special Fund for Agro-scientific Research in the Public Interest[201203065-07] ; Research Project of the Department of Education of Zhejiang Province[Y201328305] |
| WOS Research Area | Genetics & Heredity |
| WOS Subject | Genetics & Heredity |
| WOS ID | WOS:000407658000036 |
| WOS Keyword | MEDAKA ORYZIAS-LATIPES ; FLOUNDER PARALICHTHYS-OLIVACEUS ; TILAPIA OREOCHROMIS-NILOTICUS ; GONADAL SEX-DIFFERENTIATION ; TELEOST FISH ; DIMORPHIC EXPRESSION ; NILE TILAPIA ; AROMATASE IMMUNOREACTIVITY ; ACIPENSER-SINENSIS ; CHINESE STURGEON |
| Publisher | ELSEVIER SCIENCE BV |
| Funding Organization | Zhejiang Provincial Natural Science Foundation of China ; Zhejiang Provincial Natural Science Foundation of China ; Zhejiang Provincial Natural Science Foundation of China ; Zhejiang Provincial Natural Science Foundation of China ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Research Project of the Department of Education of Zhejiang Province ; Research Project of the Department of Education of Zhejiang Province ; Research Project of the Department of Education of Zhejiang Province ; Research Project of the Department of Education of Zhejiang Province ; Zhejiang Provincial Natural Science Foundation of China ; Zhejiang Provincial Natural Science Foundation of China ; Zhejiang Provincial Natural Science Foundation of China ; Zhejiang Provincial Natural Science Foundation of China ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Research Project of the Department of Education of Zhejiang Province ; Research Project of the Department of Education of Zhejiang Province ; Research Project of the Department of Education of Zhejiang Province ; Research Project of the Department of Education of Zhejiang Province ; Zhejiang Provincial Natural Science Foundation of China ; Zhejiang Provincial Natural Science Foundation of China ; Zhejiang Provincial Natural Science Foundation of China ; Zhejiang Provincial Natural Science Foundation of China ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Research Project of the Department of Education of Zhejiang Province ; Research Project of the Department of Education of Zhejiang Province ; Research Project of the Department of Education of Zhejiang Province ; Research Project of the Department of Education of Zhejiang Province ; Zhejiang Provincial Natural Science Foundation of China ; Zhejiang Provincial Natural Science Foundation of China ; Zhejiang Provincial Natural Science Foundation of China ; Zhejiang Provincial Natural Science Foundation of China ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Research Project of the Department of Education of Zhejiang Province ; Research Project of the Department of Education of Zhejiang Province ; Research Project of the Department of Education of Zhejiang Province ; Research Project of the Department of Education of Zhejiang Province |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/31448 |
| Collection | 鱼类生物学及渔业生物技术研究中心_期刊论文 |
| Corresponding Author | Huang, Wei; Lou, Bao |
| Affiliation | 1.Zhejiang Ocean Univ, Coll Marine Sci & Technol, Natl Engn Res Ctr Marine Facil Aquaculture, Zhoushan 316022, Zhejiang, Peoples R China 2.Key Lab Marine Culture & Enhancement Zhejiang Pro, Zhoushan 316022, Zhejiang, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| Recommended Citation GB/T 7714 | Huang, Wei,Yang, Pan,Lv, Zhenming,et al. Cloning, expression pattern and promoter functional analysis of cyp19a1a gene in miiuy croaker[J]. GENE,2017,627:271-277. |
| APA | Huang, Wei,Yang, Pan,Lv, Zhenming,Wu, Changwen,Gui, Jianfang,&Lou, Bao.(2017).Cloning, expression pattern and promoter functional analysis of cyp19a1a gene in miiuy croaker.GENE,627,271-277. |
| MLA | Huang, Wei,et al."Cloning, expression pattern and promoter functional analysis of cyp19a1a gene in miiuy croaker".GENE 627(2017):271-277. |
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