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The Edwardsiella piscicida Type III Effector EseJ Suppresses Expression of Type 1 Fimbriae, Leading to Decreased Bacterial Adherence to Host Cells
Zhang, Qian1,2,3; He, Tian Tian1,2,3; Li, Duan You1,2,3; Liu, Lu Yi1,2; Nie, Pin1,2,4,5; Xie, Hai Xia1,2
2019-07-01
Source PublicationINFECTION AND IMMUNITY
ISSN0019-9567
Volume87Issue:7Pages:13
Abstract

The type III secretion system (T3SS) of Edwardsiella piscicida plays a crucial role in its pathogenesis. Our previous study indicated that the T3SS effector protein EseJ inhibits the bacterium's adhesion to epithelioma papillosum cyprini (EPC) cells, while the mechanism of the inhibition remains elusive. In this study, we revealed that EseJ negatively regulates the fimA gene, as demonstrated by comparative transcription analysis of Delta eseJ and wild-type (WT) strains. As well, the dramatically increased production of FimA was detected in the absence of EseJ compared to that by the WT strain. The adherence of the Delta eseJ strain decreased far below that of the WT strain in the absence of FimA, demonstrating that FimA plays a pivotal role in the hyperadhesion of the Delta eseJ strain. Adherence analysis with a strain with truncated eseJ demonstrated that the C-terminal region of EseJ (Gly1191 to Ile1359) is necessary to inhibit the transcription of the type 1 fimbrial operon. Binding between the EseJ fragment from amino acid residues 1191 to 1359 and the DNA fragment upstream of fimA was not detected, indicating that EseJ might indirectly regulate the type 1 fimbrial operon. Our study reveals that EseJ controls E. piscicida adherence to EPC cells by negatively regulating the type 1 fimbrial operon.

KeywordEseJ T3SS type 1 fimbriae adhesion
DOI10.1128/IAI.00187-19
Funding OrganizationNational Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China (NSFC)[U1706205] ; China Agriculture Research System[CARS-46]
WOS Research AreaImmunology ; Infectious Diseases
WOS SubjectImmunology ; Infectious Diseases
WOS IDWOS:000472150800001
WOS KeywordSECRETION SYSTEM ; 2-COMPONENT SYSTEM ; CHROMOSOMAL GENES ; TARDA ; PROTEIN ; IDENTIFICATION ; VIRULENCE ; ADHESION ; COLONIZATION ; ENTRY
PublisherAMER SOC MICROBIOLOGY
Funding OrganizationNational Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/31308
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorNie, Pin; Xie, Hai Xia
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquaculture Dis Control, Minist Agr, Wuhan, Hubei, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China
5.Qingdao Agr Univ, Coll Marine Sci & Engn, Qingdao, Shandong, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Qian,He, Tian Tian,Li, Duan You,et al. The Edwardsiella piscicida Type III Effector EseJ Suppresses Expression of Type 1 Fimbriae, Leading to Decreased Bacterial Adherence to Host Cells[J]. INFECTION AND IMMUNITY,2019,87(7):13.
APA Zhang, Qian,He, Tian Tian,Li, Duan You,Liu, Lu Yi,Nie, Pin,&Xie, Hai Xia.(2019).The Edwardsiella piscicida Type III Effector EseJ Suppresses Expression of Type 1 Fimbriae, Leading to Decreased Bacterial Adherence to Host Cells.INFECTION AND IMMUNITY,87(7),13.
MLA Zhang, Qian,et al."The Edwardsiella piscicida Type III Effector EseJ Suppresses Expression of Type 1 Fimbriae, Leading to Decreased Bacterial Adherence to Host Cells".INFECTION AND IMMUNITY 87.7(2019):13.
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