IHB OpenIR  > 藻类生物学及应用研究中心  > 期刊论文
Knockdown of carbonate anhydrase elevates Nannochloropsis productivity at high CO2 level
Wei, Li1,2,5; Shen, Chen1,2,5; El Hajjami, Mohamed3; You, Wuxin1,2,3; Wang, Qintao1,2,5; Zhang, Peng1,2,5; Ji, Yuetong1,2,5; Hu, Hanhua4,5; Hu, Qiang4,5; Poetsch, Ansgar1,2,3,6; Xu, Jian1,2,5
2019-07-01
Source PublicationMETABOLIC ENGINEERING
ISSN1096-7176
Volume54Issue:1Pages:96-108
Abstract

Improving acid tolerance is pivotal to the development of microalgal feedstock for converting flue gas to biomass or oils. In the industrial oleaginous microalga Nannochloropsis oceanica, transcript knockdown of a cytosolic carbonic anhydrase (CA2), which is a key Carbon Concentrating Mechanism (CCM) component induced under 100 ppm CO2 (very low carbon, or VLC), results in similar to 45%, similar to 30% and similar to 40% elevation of photosynthetic oxygen evolution rate, growth rate and biomass accumulation rate respectively under 5% CO2 (high carbon, or HC), as compared to the wild type. Such high-CO2-level activated biomass over-production is reproducible across photobioreactor types and cultivation scales. Transcriptomic, proteomic and physiological changes of the mutant under high CO2 (HC; 5% CO2) suggest a mechanism where the higher pH tolerance is coupled to reduced biophysical CCM, sustained pH hemostasis, stimulated energy intake and enhanced photosynthesis. Thus "inactivation of CCM" can generate hyper-CO2-assimilating and autonomously containable industrial microalgae for flue gas-based oil production.

KeywordCarbon fixation Acidity tolerance Carbonic anhydrase Industrial oleaginous microalgae Nannochloropsis
DOI10.1016/j.ymben.2019.03.004
Funding OrganizationNatural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences
Indexed BySCI
Language英语
Funding ProjectNatural Science Foundation of China[31425002] ; Natural Science Foundation of China[31741005] ; Biological Carbon Sequestration Program of Chinese Academy of Sciences[KSZD-EW-Z-017] ; Biological Carbon Sequestration Program of Chinese Academy of Sciences[ZDRW-ZS-2016-3]
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000470680100008
WOS KeywordOLEAGINOUS MICROALGAE ; CO2-CONCENTRATING MECHANISM ; CHLAMYDOMONAS-REINHARDTII ; CONCENTRATING MECHANISMS ; COMPUTATIONAL PLATFORM ; MODEL ; PHOTOSYNTHESIS ; BIOSYNTHESIS ; ACCLIMATION ; MODULATION
PublisherACADEMIC PRESS INC ELSEVIER SCIENCE
Funding OrganizationNatural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences ; Biological Carbon Sequestration Program of Chinese Academy of Sciences
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/31258
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorWei, Li; Poetsch, Ansgar; Xu, Jian
Affiliation1.Chinese Acad Sci, Single Cell Ctr, CAS Key Lab Biofuels, Qingdao, Shandong, Peoples R China
2.Chinese Acad Sci, Shandong Key Lab Energy Genet, Qingdao Inst BioEnergy & Bioproc Technol, Qingdao, Shandong, Peoples R China
3.Ruhr Univ Bochum, Dept Plant Biochem, Bochum, Germany
4.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Hubei, Peoples R China
5.Univ Chinese Acad Sci, Beijing, Peoples R China
6.Univ Plymouth, Sch Biomed & Healthcare Sci, Plymouth, Devon, England
Recommended Citation
GB/T 7714
Wei, Li,Shen, Chen,El Hajjami, Mohamed,et al. Knockdown of carbonate anhydrase elevates Nannochloropsis productivity at high CO2 level[J]. METABOLIC ENGINEERING,2019,54(1):96-108.
APA Wei, Li.,Shen, Chen.,El Hajjami, Mohamed.,You, Wuxin.,Wang, Qintao.,...&Xu, Jian.(2019).Knockdown of carbonate anhydrase elevates Nannochloropsis productivity at high CO2 level.METABOLIC ENGINEERING,54(1),96-108.
MLA Wei, Li,et al."Knockdown of carbonate anhydrase elevates Nannochloropsis productivity at high CO2 level".METABOLIC ENGINEERING 54.1(2019):96-108.
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