Molecular characterization and expression regulation of the factor-inhibiting HIF-1 (FIH-1) gene under hypoxic stress in bighead carp (Aristichthys nobilis) | |
Feng, Xiu1; Yu, Xiaomu1; Pang, Meixia2; Tong, Jingou1 | |
2019-04-01 | |
Source Publication | FISH PHYSIOLOGY AND BIOCHEMISTRY
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ISSN | 0920-1742 |
Volume | 45Issue:2Pages:657-665 |
Abstract | Factor-inhibiting HIF-1 (FIH-1) is an asparagine hydroxylase that interacts with hypoxia-inducible factor 1 (HIF-1) to regulate transcriptional activity of HIF-1. Few studies of fish FIH-1 have been reported to date. In this study, the cDNA of FIH-1 gene was cloned and characterized for bighead carp, Aristichthys nobilis (AnFIH-1). The AnFIH-1 cDNA is 2065bp in length, encoding a protein of 357 amino acid (aa) residues, which contains a JmjC homology region of the jumonji transcription factors. AnFIH-1 shares high identities with other vertebrate FIH-1 (79.1-96.4%), especially in the JmjC homology region, suggesting its conserved function. During the embryonic stages of A. nobilis, AnFIH-1 had significantly high expression levels in unfertilized egg and blastula. In healthy tissues, its predominant mRNA expression was detected in muscle. The mRNA levels of AnFIH-1 were significantly upregulated in the liver, gill, hypothalamus, and spleen after hypoxic treatment, and then decreased to pretreatment levels after 6-h re-oxygenation. However, in the muscle, continual increasing of mRNA expression was observed after hypoxic shock and re-oxygenation. These results indicate that FIH-1 may play an important role in physiological regulation for adapting to hypoxia stress in A. nobilis. |
Keyword | Aristichthys nobilis Factor-inhibiting HIF-1 Gene expression Hypoxia |
DOI | 10.1007/s10695-018-0597-7 |
Funding Organization | NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC |
Indexed By | SCI |
Language | 英语 |
Funding Project | NSFC[31272647] ; NSFC[31472268] |
WOS Research Area | Biochemistry & Molecular Biology ; Fisheries ; Physiology |
WOS Subject | Biochemistry & Molecular Biology ; Fisheries ; Physiology |
WOS ID | WOS:000466931600014 |
WOS Keyword | FACTOR-ALPHA SUBUNITS ; INDUCIBLE FACTOR HIF ; HIF-1-ALPHA ; PROTEIN ; CLONING ; VHL ; OVEREXPRESSION ; RESPONSES ; ISOFORMS ; DISTINCT |
Publisher | SPRINGER |
Funding Organization | NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.ihb.ac.cn/handle/342005/31246 |
Collection | 鱼类生物学及渔业生物技术研究中心_期刊论文 |
Corresponding Author | Tong, Jingou |
Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 2.Shenzhen Polytech, Sch Appl Chem & Biol Technol, Shenzhen 518055, Peoples R China |
Recommended Citation GB/T 7714 | Feng, Xiu,Yu, Xiaomu,Pang, Meixia,et al. Molecular characterization and expression regulation of the factor-inhibiting HIF-1 (FIH-1) gene under hypoxic stress in bighead carp (Aristichthys nobilis)[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2019,45(2):657-665. |
APA | Feng, Xiu,Yu, Xiaomu,Pang, Meixia,&Tong, Jingou.(2019).Molecular characterization and expression regulation of the factor-inhibiting HIF-1 (FIH-1) gene under hypoxic stress in bighead carp (Aristichthys nobilis).FISH PHYSIOLOGY AND BIOCHEMISTRY,45(2),657-665. |
MLA | Feng, Xiu,et al."Molecular characterization and expression regulation of the factor-inhibiting HIF-1 (FIH-1) gene under hypoxic stress in bighead carp (Aristichthys nobilis)".FISH PHYSIOLOGY AND BIOCHEMISTRY 45.2(2019):657-665. |
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