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Molecular characterization and expression regulation of the factor-inhibiting HIF-1 (FIH-1) gene under hypoxic stress in bighead carp (Aristichthys nobilis)
Feng, Xiu1; Yu, Xiaomu1; Pang, Meixia2; Tong, Jingou1
2019-04-01
Source PublicationFISH PHYSIOLOGY AND BIOCHEMISTRY
ISSN0920-1742
Volume45Issue:2Pages:657-665
Abstract

Factor-inhibiting HIF-1 (FIH-1) is an asparagine hydroxylase that interacts with hypoxia-inducible factor 1 (HIF-1) to regulate transcriptional activity of HIF-1. Few studies of fish FIH-1 have been reported to date. In this study, the cDNA of FIH-1 gene was cloned and characterized for bighead carp, Aristichthys nobilis (AnFIH-1). The AnFIH-1 cDNA is 2065bp in length, encoding a protein of 357 amino acid (aa) residues, which contains a JmjC homology region of the jumonji transcription factors. AnFIH-1 shares high identities with other vertebrate FIH-1 (79.1-96.4%), especially in the JmjC homology region, suggesting its conserved function. During the embryonic stages of A. nobilis, AnFIH-1 had significantly high expression levels in unfertilized egg and blastula. In healthy tissues, its predominant mRNA expression was detected in muscle. The mRNA levels of AnFIH-1 were significantly upregulated in the liver, gill, hypothalamus, and spleen after hypoxic treatment, and then decreased to pretreatment levels after 6-h re-oxygenation. However, in the muscle, continual increasing of mRNA expression was observed after hypoxic shock and re-oxygenation. These results indicate that FIH-1 may play an important role in physiological regulation for adapting to hypoxia stress in A. nobilis.

KeywordAristichthys nobilis Factor-inhibiting HIF-1 Gene expression Hypoxia
DOI10.1007/s10695-018-0597-7
Funding OrganizationNSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC
Indexed BySCI
Language英语
Funding ProjectNSFC[31272647] ; NSFC[31472268]
WOS Research AreaBiochemistry & Molecular Biology ; Fisheries ; Physiology
WOS SubjectBiochemistry & Molecular Biology ; Fisheries ; Physiology
WOS IDWOS:000466931600014
WOS KeywordFACTOR-ALPHA SUBUNITS ; INDUCIBLE FACTOR HIF ; HIF-1-ALPHA ; PROTEIN ; CLONING ; VHL ; OVEREXPRESSION ; RESPONSES ; ISOFORMS ; DISTINCT
PublisherSPRINGER
Funding OrganizationNSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/31246
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorTong, Jingou
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Shenzhen Polytech, Sch Appl Chem & Biol Technol, Shenzhen 518055, Peoples R China
Recommended Citation
GB/T 7714
Feng, Xiu,Yu, Xiaomu,Pang, Meixia,et al. Molecular characterization and expression regulation of the factor-inhibiting HIF-1 (FIH-1) gene under hypoxic stress in bighead carp (Aristichthys nobilis)[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2019,45(2):657-665.
APA Feng, Xiu,Yu, Xiaomu,Pang, Meixia,&Tong, Jingou.(2019).Molecular characterization and expression regulation of the factor-inhibiting HIF-1 (FIH-1) gene under hypoxic stress in bighead carp (Aristichthys nobilis).FISH PHYSIOLOGY AND BIOCHEMISTRY,45(2),657-665.
MLA Feng, Xiu,et al."Molecular characterization and expression regulation of the factor-inhibiting HIF-1 (FIH-1) gene under hypoxic stress in bighead carp (Aristichthys nobilis)".FISH PHYSIOLOGY AND BIOCHEMISTRY 45.2(2019):657-665.
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