IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Molecular and functional characterization of the indoleamine 2,3-dioxygenase in grass carp (Ctenopharyngodon idella)
Cui, Zheng-Wei1,4; Zhang, Xiang-Yang1,4; Zhang, Xu-Jie2; Wu, Nan1; Lu, Long-Feng1; Li, Shun1; Chen, Dan-Dan1; Zhang, Yong-An1,2,3
Corresponding AuthorZhang, Yong-An(yzhang@ihb.ac.cn)
2019-06-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume89Pages:301-308
AbstractIndoleamine 2,3-dioxygenase (IDO) is a kind of dioxygenase that can catalyze the degradation of levo-tryptophan (L-Trp) and plays key roles in immune tolerance. In this study, the DO gene was cloned and functionally characterized from grass carp (gcIDO). The results showed that gcIDO overexpressed in GCO cells could catalyze the degradation of L-Trp through the L-Trp kynurenine pathway, and this activity could be promoted by delta-aminolevulinic acid (ALA) while inhibited by levo-1-methyl tryptophan (L-1MT). Moreover, gcIDO was constitutively expressed in various tissues, and its expression could be significantly up-regulated by LPS and Poly (I:C) in peripheral blood leukocytes (Pins). Furthermore, recombinant TGF-beta 1 of grass carp could up-regulate the expression of IDO, TGF-beta 1, CD25, and Foxp3 in PBLs, indicating that the TGF-beta 1/IDO pathway is present in fish. In the soybean meal induced enteritis (SBMIE) model, the expression of gcIDO in the intestine was upregulated significantly, demonstrating that gcIDO may play an immunoregulatory role in SBMIE. Taken together, these data suggest that the IDO plays multiple roles in the immunity of fish.
KeywordIndoleamine 2,3-dioxygenase (IDO) Tryptophan TGF-beta 1 Immunoregulation Grass carp
DOI10.1016/j.fsi.2019.04.005
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China
Indexed BySCI ; SCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31472307] ; National Basic Research Program of China[2014CB138601]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000469897700034
WOS KeywordGENE-EXPRESSION ; RAINBOW-TROUT ; TRYPTOPHAN ; CELLS ; TOLERANCE ; LIPOPOLYSACCHARIDE ; INDUCTION ; PROTEIN ; BETA ; IDO
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/31212
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorZhang, Yong-An
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Hubei, Peoples R China
2.Huazhong Agr Univ, Coll Fisheries, State Key Lab Agr Microbiol, Wuhan, Hubei, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China
4.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Cui, Zheng-Wei,Zhang, Xiang-Yang,Zhang, Xu-Jie,et al. Molecular and functional characterization of the indoleamine 2,3-dioxygenase in grass carp (Ctenopharyngodon idella)[J]. FISH & SHELLFISH IMMUNOLOGY,2019,89:301-308.
APA Cui, Zheng-Wei.,Zhang, Xiang-Yang.,Zhang, Xu-Jie.,Wu, Nan.,Lu, Long-Feng.,...&Zhang, Yong-An.(2019).Molecular and functional characterization of the indoleamine 2,3-dioxygenase in grass carp (Ctenopharyngodon idella).FISH & SHELLFISH IMMUNOLOGY,89,301-308.
MLA Cui, Zheng-Wei,et al."Molecular and functional characterization of the indoleamine 2,3-dioxygenase in grass carp (Ctenopharyngodon idella)".FISH & SHELLFISH IMMUNOLOGY 89(2019):301-308.
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