| Differential expression of innate and adaptive immune genes in the survivors of three gibel carp gynogenetic clones after herpesvirus challenge | |
| Lu, Wei-Jia1,2; Gao, Fan-Xiang1,2,3; Wang, Yang1,2; Zhang, Qi-Ya1,2; Li, Zhi1; Zhang, Xiao-Juan1; Zhou, Li1,2; Gui, Jian-Fang1,2 | |
| Corresponding Author | Zhou, Li(zhouli@ihb.ac.cn) ; Gui, Jian-Fang(jfgui@ihb.ac.cn) |
| 2019-05-28 | |
| Source Publication | BMC GENOMICS
 |
| ISSN | 1471-2164 |
| Volume | 20Pages:19 |
| Abstract | BackgroundAccompanied with rapid growth and high density aquaculture, gibel carp has been seriously threatened by Carassius auratus herpesvirus (CaHV) since 2012. In previous study, distinct CaHV resistances and immune responses were revealed in the diseased individuals of three gibel carp gynogenetic clones (A(+), F and H). However, little is known about the gene expression changes in the survivors after CaHV challenge, particularly their differences of innate and adaptive immune system between susceptible clone and resistant clone.ResultsWe firstly confirmed the CaHV carrier state in the survivors of three gibel carp clones after CaHV challenge by evaluating the abundances of five CaHV genes. The assay of viral loads indicated the resistant clone H possessed not only stronger resistance but also higher tolerance to CaHV. Then, 2818, 4047 and 3323 differentially expressed unigenes (DEUs) were screened from the head-kidney transcriptome profiles of survivors compared with controls from clone A(+), F and H. GO and KEGG analysis suggested that a persistent immune response might sustain in resistant clone H and F, while susceptible clone A(+) had a long-term impact on the circulatory system which was consistent with the major symptoms of bleeding caused by CaHV. Among the top 30 enriched pathways of specifically up-regulated DEUs in respective clones, 26, 7 and 15 pathways in clone H, F and A(+) were associated with infections, diseases, or immune-related pathways respectively. In addition, 20 pathways in clone F belonged to metabolism or biogenesis, and 7 pathways involved in circulatory system were enriched in clone A(+). Significantly, we revealed the differential expression changes of IFN system genes and immunoglobulin (Ig) genes among the survivors of three clones. Finally, myosins and Igs were identified as co-expression modules which were positively or negatively correlated to CaHV viral loads respectively.ConclusionsOur results revealed the common and distinct gene expression changes in immune and circulatory system in the survivors of three gibel carp gynogenetic clones with different CaHV resistances. The current study represents a paradigm of differential innate and adaptive immune reactions in teleost, and will be beneficial to the disease-resistance breeding of gibel carp. |
| Keyword | Gibel carp Herpesvirus Transcriptome Survivors IFN Immunoglobulin Myosin |
| DOI | 10.1186/s12864-019-5777-z |
| Funding Organization | Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System |
| Indexed By | SCI ; SCI |
| Language | 英语 |
| Funding Project | Strategic Priority Research Program of the Chinese Academy of Sciences[XDA08030202] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA08030201] ; National Natural Science Foundation[31772838] ; earmarked fund for Modern Agro-industry Technology Research System[NYCYTX-49] |
| WOS Research Area | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| WOS Subject | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| WOS ID | WOS:000469326100006 |
| WOS Keyword | CARASSIUS-AURATUS-GIBELIO ; EXTRA MICROCHROMOSOMES ; ANTIVIRAL RESPONSE ; DISEASE TOLERANCE ; COMPLETE GENOME ; RNA-SEQ ; INTERFERON ; VIRUS ; FISH ; SYSTEM |
| Publisher | BMC |
| Funding Organization | Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/31203 |
| Collection | 鱼类生物学及渔业生物技术研究中心_期刊论文 |
| Corresponding Author | Zhou, Li; Gui, Jian-Fang |
| Affiliation | 1.Chinese Acad Sci, Innovat Acad Seed Design, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Hohai Univ, Coll Oceanog, Inst Marine Biol, Nanjing 210098, Jiangsu, Peoples R China |
| Recommended Citation GB/T 7714 | Lu, Wei-Jia,Gao, Fan-Xiang,Wang, Yang,et al. Differential expression of innate and adaptive immune genes in the survivors of three gibel carp gynogenetic clones after herpesvirus challenge[J]. BMC GENOMICS,2019,20:19. |
| APA | Lu, Wei-Jia.,Gao, Fan-Xiang.,Wang, Yang.,Zhang, Qi-Ya.,Li, Zhi.,...&Gui, Jian-Fang.(2019).Differential expression of innate and adaptive immune genes in the survivors of three gibel carp gynogenetic clones after herpesvirus challenge.BMC GENOMICS,20,19. |
| MLA | Lu, Wei-Jia,et al."Differential expression of innate and adaptive immune genes in the survivors of three gibel carp gynogenetic clones after herpesvirus challenge".BMC GENOMICS 20(2019):19. |
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