IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Steroidogenic factor-1 hypermethylation in maternal rat blood could serve as a biomarker for intrauterine growth retardation
Wu, Dong-Mei1; Ma, Liang-Peng1,3; Song, Gui-Li2; Long, Yong2; Liu, Han-Xiao1; Liu, Yang1; Ping, Jie1
2017-11-10
Source PublicationONCOTARGET
ISSN1949-2553
Volume8Issue:56Pages:96139-96153
Abstract

Intrauterine growth retardation (IUGR) is a common obstetric complication lacking an optimal method for prenatal screening. DNA methylation profile in maternal blood holds significant promise for prenatal screening. Here, we aimed to screen out potential IUGR biomarkers in maternal blood from the perspective of DNA methylation. The IUGR rat model was established by prenatal maternal undernutrition. High-throughput bisulfite sequencing of genomic DNA methylation followed by functional clustering analysis for differentially methylated region (DMR)-associated genes demonstrated that genes regulating transcription had the most significantly changed DNA methylation status in maternal blood with IUGR. Genes about apoptosis and placental development were also changed. Besides increased placental apoptosis, IUGR rats demonstrated the same hypermethylated CpG sites of steroidogenic factor-1 (SF-1, a DMR-associated transcription factor about placenta) promoter in maternal blood and placentae. Further, ff1b, the SF-1 ortholog, was knocked out in zebrafish by CRISPR/Cas9 technology. The knock-out zebrafish demonstrated developmental inhibition and increased IUGR rates, which confirmed the role of SF-1 in IUGR development. Finally, hypermethylated SF-1 was observed in human maternal blood of IUGR. This study firstly presented distinct DNA methylation profile in maternal blood of IUGR and showed hypermethylated SF-1 could be a potential IUGR biomarker in maternal rat blood.

Keywordsteroidogenic factor-1 (SF-1) DNA methylation intrauterine growth retardation (IUGR) clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) biomarker
DOI10.18632/oncotarget.21767
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[81673215] ; National Natural Science Foundation of China[81273107] ; Applied Fundamental Research Project of Wuhan[2017060201010199]
WOS Research AreaOncology ; Cell Biology
WOS SubjectOncology ; Cell Biology
WOS IDWOS:000414937900086
WOS KeywordFETAL ADRENAL STEROIDOGENESIS ; DNA METHYLATION ; EPIGENETIC ALTERATIONS ; HISTONE ACETYLATION ; FOOD RESTRICTION ; OFFSPRING RATS ; EXPRESSION ; IUGR ; ASSOCIATION ; PREGNANCY
PublisherIMPACT JOURNALS LLC
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/30810
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorPing, Jie
Affiliation1.Wuhan Univ, Sch Basic Med Sci, Dept Pharmacol, Wuhan 430071, Hubei, Peoples R China
2.Chinese Acad Sci, Key Lab Biodivers & Conservat Aquat Organism, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
3.Wuhan First Hosp, Dept Pharm, Wuhan 430022, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Wu, Dong-Mei,Ma, Liang-Peng,Song, Gui-Li,et al. Steroidogenic factor-1 hypermethylation in maternal rat blood could serve as a biomarker for intrauterine growth retardation[J]. ONCOTARGET,2017,8(56):96139-96153.
APA Wu, Dong-Mei.,Ma, Liang-Peng.,Song, Gui-Li.,Long, Yong.,Liu, Han-Xiao.,...&Ping, Jie.(2017).Steroidogenic factor-1 hypermethylation in maternal rat blood could serve as a biomarker for intrauterine growth retardation.ONCOTARGET,8(56),96139-96153.
MLA Wu, Dong-Mei,et al."Steroidogenic factor-1 hypermethylation in maternal rat blood could serve as a biomarker for intrauterine growth retardation".ONCOTARGET 8.56(2017):96139-96153.
Files in This Item: Download All
File Name/Size DocType Version Access License
Wu-2017-Steroidogeni(3968KB)期刊论文出版稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wu, Dong-Mei]'s Articles
[Ma, Liang-Peng]'s Articles
[Song, Gui-Li]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wu, Dong-Mei]'s Articles
[Ma, Liang-Peng]'s Articles
[Song, Gui-Li]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wu, Dong-Mei]'s Articles
[Ma, Liang-Peng]'s Articles
[Song, Gui-Li]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Wu-2017-Steroidogenic factor-1 hypermethylatio.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.