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The Type IX Secretion System Is Required for Virulence of the Fish Pathogen Flavobacterium columnare
Li, Nan1,2; Zhu, Yongtao2; LaFrentz, Benjamin R.3; Evenhuis, Jason P.4; Hunnicutt, David W.5; Conrad, Rachel A.2; Barbier, Paul2; Gullstrand, Connor W.5; Roets, Jack E.5; Powers, Jonathan L.5; Kulkarni, Surashree S.2; Erbes, Devon H.2; Garcia, Julio C.3; Nie, Pin1; McBride, Mark J.2
Corresponding AuthorNie, Pin(pinnie@ihb.ac.cn) ; McBride, Mark J.(mcbride@uwm.edu)
2017-12-01
Source PublicationAPPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN0099-2240
Volume83Issue:23Pages:17
AbstractFlavobacterium columnare, a member of the phylum Bacteroidetes, causes columnaris disease in wild and aquaculture-reared freshwater fish. The mechanisms responsible for columnaris disease are not known. Many members of the phylum Bacteroidetes use type IX secretion systems (T9SSs) to secrete enzymes, adhesins, and proteins involved in gliding motility. The F. columnare genome has all of the genes needed to encode a T9SS. gldN, which encodes a core component of the T9SS, was deleted in wild-type strains of F. columnare. The F. columnare Delta gldN mutants were deficient in the secretion of several extracellular proteins and lacked gliding motility. The Delta gldN mutants exhibited reduced virulence in zebrafish, channel catfish, and rainbow trout, and complementation restored virulence. PorV is required for the secretion of a subset of proteins targeted to the T9SS. An F. columnare Delta porV mutant retained gliding motility but exhibited reduced virulence. Cell-free spent media from exponentially growing cultures of wild-type and complemented strains caused rapid mortality, but spent media from Delta gldN and Delta porV mutants did not, suggesting that soluble toxins are secreted by the T9SS. IMPORTANCE Columnaris disease, caused by F. columnare, is a major problem for freshwater aquaculture. Little is known regarding the virulence factors produced by F. columnare, and control measures are limited. Analysis of targeted gene deletion mutants revealed the importance of the type IX protein secretion system (T9SS) and of secreted toxins in F. columnare virulence. T9SSs are common in members of the phylum Bacteroidetes and likely contribute to the virulence of other animal and human pathogens.
Keywordcolumnaris disease gliding motility type IX secretion system virulence
DOI10.1128/AEM.01769-17
Funding OrganizationUSDA-ARS (CRIS) ; USDA-ARS (CRIS) ; USDA-ARS (CRIS) ; USDA-ARS (CRIS) ; USDA-ARS ; USDA-ARS ; USDA-ARS ; USDA-ARS ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Wisconsin Sea Grant Institute from National Sea Grant College Program, National Oceanic and Atmospheric Administration, U.S. Department of Commerce ; University of Wisconsin Sea Grant Institute from National Sea Grant College Program, National Oceanic and Atmospheric Administration, U.S. Department of Commerce ; University of Wisconsin Sea Grant Institute from National Sea Grant College Program, National Oceanic and Atmospheric Administration, U.S. Department of Commerce ; University of Wisconsin Sea Grant Institute from National Sea Grant College Program, National Oceanic and Atmospheric Administration, U.S. Department of Commerce ; State of Wisconsin, federal grant ; State of Wisconsin, federal grant ; State of Wisconsin, federal grant ; State of Wisconsin, federal grant ; USDA-ARS (CRIS) ; USDA-ARS (CRIS) ; USDA-ARS (CRIS) ; USDA-ARS (CRIS) ; USDA-ARS ; USDA-ARS ; USDA-ARS ; USDA-ARS ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Wisconsin Sea Grant Institute from National Sea Grant College Program, National Oceanic and Atmospheric Administration, U.S. Department of Commerce ; University of Wisconsin Sea Grant Institute from National Sea Grant College Program, National Oceanic and Atmospheric Administration, U.S. Department of Commerce ; University of Wisconsin Sea Grant Institute from National Sea Grant College Program, National Oceanic and Atmospheric Administration, U.S. Department of Commerce ; University of Wisconsin Sea Grant Institute from National Sea Grant College Program, National Oceanic and Atmospheric Administration, U.S. Department of Commerce ; State of Wisconsin, federal grant ; State of Wisconsin, federal grant ; State of Wisconsin, federal grant ; State of Wisconsin, federal grant ; USDA-ARS (CRIS) ; USDA-ARS (CRIS) ; USDA-ARS (CRIS) ; USDA-ARS (CRIS) ; USDA-ARS ; USDA-ARS ; USDA-ARS ; USDA-ARS ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Wisconsin Sea Grant Institute from National Sea Grant College Program, National Oceanic and Atmospheric Administration, U.S. Department of Commerce ; University of Wisconsin Sea Grant Institute from National Sea Grant College Program, National Oceanic and Atmospheric Administration, U.S. Department of Commerce ; University of Wisconsin Sea Grant Institute from National Sea Grant College Program, National Oceanic and Atmospheric Administration, U.S. Department of Commerce ; University of Wisconsin Sea Grant Institute from National Sea Grant College Program, National Oceanic and Atmospheric Administration, U.S. Department of Commerce ; State of Wisconsin, federal grant ; State of Wisconsin, federal grant ; State of Wisconsin, federal grant ; State of Wisconsin, federal grant ; USDA-ARS (CRIS) ; USDA-ARS (CRIS) ; USDA-ARS (CRIS) ; USDA-ARS (CRIS) ; USDA-ARS ; USDA-ARS ; USDA-ARS ; USDA-ARS ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Wisconsin Sea Grant Institute from National Sea Grant College Program, National Oceanic and Atmospheric Administration, U.S. Department of Commerce ; University of Wisconsin Sea Grant Institute from National Sea Grant College Program, National Oceanic and Atmospheric Administration, U.S. Department of Commerce ; University of Wisconsin Sea Grant Institute from National Sea Grant College Program, National Oceanic and Atmospheric Administration, U.S. Department of Commerce ; University of Wisconsin Sea Grant Institute from National Sea Grant College Program, National Oceanic and Atmospheric Administration, U.S. Department of Commerce ; State of Wisconsin, federal grant ; State of Wisconsin, federal grant ; State of Wisconsin, federal grant ; State of Wisconsin, federal grant
Indexed BySCI ; SCI
Language英语
Funding ProjectUSDA-ARS (CRIS)[6010-32000-026-00D] ; USDA-ARS[5090-31320-004-03S] ; National Science Foundation[MCB-1516990] ; Chinese Academy of Sciences[XDA08010207] ; Chinese Academy of Sciences ; University of Wisconsin Sea Grant Institute from National Sea Grant College Program, National Oceanic and Atmospheric Administration, U.S. Department of Commerce ; State of Wisconsin, federal grant[NA14OAR4170092] ; State of Wisconsin, federal grant[R/SFA-08] ; State of Wisconsin, federal grant[R/SFA-11]
WOS Research AreaBiotechnology & Applied Microbiology ; Microbiology
WOS SubjectBiotechnology & Applied Microbiology ; Microbiology
WOS IDWOS:000415617800021
WOS KeywordTROUT ONCORHYNCHUS-MYKISS ; GRAM-NEGATIVE BACTERIA ; CATFISH ICTALURUS-PUNCTATUS ; C-TERMINAL-DOMAIN ; AC LYASE ACTIVITY ; GLIDING MOTILITY ; PORPHYROMONAS-GINGIVALIS ; PROTEIN SECRETION ; CHANNEL CATFISH ; FLEXIBACTER-COLUMNARIS
PublisherAMER SOC MICROBIOLOGY
Funding OrganizationUSDA-ARS (CRIS) ; USDA-ARS (CRIS) ; USDA-ARS (CRIS) ; USDA-ARS (CRIS) ; USDA-ARS ; USDA-ARS ; USDA-ARS ; USDA-ARS ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Wisconsin Sea Grant Institute from National Sea Grant College Program, National Oceanic and Atmospheric Administration, U.S. Department of Commerce ; University of Wisconsin Sea Grant Institute from National Sea Grant College Program, National Oceanic and Atmospheric Administration, U.S. Department of Commerce ; University of Wisconsin Sea Grant Institute from National Sea Grant College Program, National Oceanic and Atmospheric Administration, U.S. Department of Commerce ; University of Wisconsin Sea Grant Institute from National Sea Grant College Program, National Oceanic and Atmospheric Administration, U.S. Department of Commerce ; State of Wisconsin, federal grant ; State of Wisconsin, federal grant ; State of Wisconsin, federal grant ; State of Wisconsin, federal grant ; USDA-ARS (CRIS) ; USDA-ARS (CRIS) ; USDA-ARS (CRIS) ; USDA-ARS (CRIS) ; USDA-ARS ; USDA-ARS ; USDA-ARS ; USDA-ARS ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Wisconsin Sea Grant Institute from National Sea Grant College Program, National Oceanic and Atmospheric Administration, U.S. Department of Commerce ; University of Wisconsin Sea Grant Institute from National Sea Grant College Program, National Oceanic and Atmospheric Administration, U.S. Department of Commerce ; University of Wisconsin Sea Grant Institute from National Sea Grant College Program, National Oceanic and Atmospheric Administration, U.S. Department of Commerce ; University of Wisconsin Sea Grant Institute from National Sea Grant College Program, National Oceanic and Atmospheric Administration, U.S. Department of Commerce ; State of Wisconsin, federal grant ; State of Wisconsin, federal grant ; State of Wisconsin, federal grant ; State of Wisconsin, federal grant ; USDA-ARS (CRIS) ; USDA-ARS (CRIS) ; USDA-ARS (CRIS) ; USDA-ARS (CRIS) ; USDA-ARS ; USDA-ARS ; USDA-ARS ; USDA-ARS ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Wisconsin Sea Grant Institute from National Sea Grant College Program, National Oceanic and Atmospheric Administration, U.S. Department of Commerce ; University of Wisconsin Sea Grant Institute from National Sea Grant College Program, National Oceanic and Atmospheric Administration, U.S. Department of Commerce ; University of Wisconsin Sea Grant Institute from National Sea Grant College Program, National Oceanic and Atmospheric Administration, U.S. Department of Commerce ; University of Wisconsin Sea Grant Institute from National Sea Grant College Program, National Oceanic and Atmospheric Administration, U.S. Department of Commerce ; State of Wisconsin, federal grant ; State of Wisconsin, federal grant ; State of Wisconsin, federal grant ; State of Wisconsin, federal grant ; USDA-ARS (CRIS) ; USDA-ARS (CRIS) ; USDA-ARS (CRIS) ; USDA-ARS (CRIS) ; USDA-ARS ; USDA-ARS ; USDA-ARS ; USDA-ARS ; National Science Foundation ; National Science Foundation ; National Science Foundation ; National Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Wisconsin Sea Grant Institute from National Sea Grant College Program, National Oceanic and Atmospheric Administration, U.S. Department of Commerce ; University of Wisconsin Sea Grant Institute from National Sea Grant College Program, National Oceanic and Atmospheric Administration, U.S. Department of Commerce ; University of Wisconsin Sea Grant Institute from National Sea Grant College Program, National Oceanic and Atmospheric Administration, U.S. Department of Commerce ; University of Wisconsin Sea Grant Institute from National Sea Grant College Program, National Oceanic and Atmospheric Administration, U.S. Department of Commerce ; State of Wisconsin, federal grant ; State of Wisconsin, federal grant ; State of Wisconsin, federal grant ; State of Wisconsin, federal grant
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/30807
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorNie, Pin; McBride, Mark J.
Affiliation1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Hubei, Peoples R China
2.Univ Wisconsin Milwaukee, Dept Biol Sci, Milwaukee, WI 53211 USA
3.USDA ARS, Aquat Anim Hlth Res Unit, Auburn, AL USA
4.USDA ARS, Natl Ctr Cool & Cold Water Aquaculture, Kearneysville, WV USA
5.St Norbert Coll, Dept Biol, De Pere, WI 54115 USA
Recommended Citation
GB/T 7714
Li, Nan,Zhu, Yongtao,LaFrentz, Benjamin R.,et al. The Type IX Secretion System Is Required for Virulence of the Fish Pathogen Flavobacterium columnare[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2017,83(23):17.
APA Li, Nan.,Zhu, Yongtao.,LaFrentz, Benjamin R..,Evenhuis, Jason P..,Hunnicutt, David W..,...&McBride, Mark J..(2017).The Type IX Secretion System Is Required for Virulence of the Fish Pathogen Flavobacterium columnare.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,83(23),17.
MLA Li, Nan,et al."The Type IX Secretion System Is Required for Virulence of the Fish Pathogen Flavobacterium columnare".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 83.23(2017):17.
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