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RpoN (sigma(54)) Is Required for Floc Formation but Not for Extracellular Polysaccharide Biosynthesis in a Floc-Forming Aquincola tertiaricarbonis Strain
Yu, Dianzhen1,2; Xia, Ming1,2; Zhang, Liping1; Song, Yulong1; Duan, You1,2; Yuan, Tong4,6; Yao, Minjie3; Wu, Liyou4; Tian, Chunyuan5; Wu, Zhenbin1; Li, Xiangzhen3; Zhou, Jizhong4; Qiu, Dongru1
2017-07-01
Source PublicationAPPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN0099-2240
Volume83Issue:14Pages:17
Abstract

Some bacteria are capable of forming flocs, in which bacterial cells become self-flocculated by secreted extracellular polysaccharides and other biopolymers. The floc-forming bacteria play a central role in activated sludge, which has been widely utilized for the treatment of municipal sewage and industrial waste-water. Here, we use a floc-forming bacterium, Aquincola tertiaricarbonis RN12, as a model to explore the biosynthesis of extracellular polysaccharides and the regulation of floc formation. A large gene cluster for exopolysaccharide biosynthesis and a gene encoding the alternative sigma factor RpoN1, one of the four paralogues, have been identified in floc formation-deficient mutants generated by transposon mutagenesis, and the gene functions have been further confirmed by genetic complementation analyses. Interestingly, the biosynthesis of exopolysaccharides remained in the rpoN1-disrupted flocculation-defective mutants, but most of the exopolysaccharides were secreted and released rather than bound to the cells. Furthermore, the expression of exopolysaccharide biosynthesis genes seemed not to be regulated by RpoN1. Taken together, our results indicate that RpoN1 may play a role in regulating the expression of a certain gene(s) involved in the self-flocculation of bacterial cells but not in the biosynthesis and secretion of exopolysaccharides required for floc formation. IMPORTANCE Floc formation confers bacterial resistance to predation of protozoa and plays a central role in the widely used activated sludge process. In this study, we not only identified a large gene cluster for biosynthesis of extracellular polysaccharides but also identified four rpoN paralogues, one of which (rpoN1) is required for floc formation in A. tertiaricarbonis RN12. In addition, this RpoN sigma factor regulates the transcription of genes involved in biofilm formation and swarming motility, as previously shown in other bacteria. However, this RpoN paralogue is not required for the biosynthesis of exopolysaccharides, which are released and dissolved into culture broth by the rpoN1 mutant rather than remaining tightly bound to cells, as observed during the flocculation of the wild-type strain. These results indicate that floc formation is a regulated complex process, and other yet-to-be identified RpoN1-dependent factors are involved in self-flocculation of bacterial cells via exopolysaccharides and/or other biopolymers.

Keywordbacterial floc formation extracellular polysaccharides flocculation RpoN sigma factor Aquincola tertiaricarbonis
DOI10.1128/AEM.00709-17
Funding OrganizationChinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award
Indexed BySCI
Language英语
Funding ProjectChinese Academy of Science[Y15103-1-401] ; One-Hundred Scholar Award
WOS Research AreaBiotechnology & Applied Microbiology ; Microbiology
WOS SubjectBiotechnology & Applied Microbiology ; Microbiology
WOS IDWOS:000415759800001
WOS KeywordPSEUDOMONAS-AERUGINOSA ; BIOFILM FORMATION ; TWITCHING MOTILITY ; GENE-EXPRESSION ; IV PILI ; ACINETOBACTER-CALCOACETICUS ; RHODOBACTER-SPHAEROIDES ; DIFFERENTIAL REGULATION ; RALSTONIA-SOLANACEARUM ; SHEWANELLA-ONEIDENSIS
PublisherAMER SOC MICROBIOLOGY
Funding OrganizationChinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; One-Hundred Scholar Award
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/30739
Collection水环境工程研究中心_期刊论文
Corresponding AuthorQiu, Dongru
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Chinese Acad Sci, Chengdu Inst Biol, Chengdu, Sichuan, Peoples R China
4.Univ Oklahoma, Inst Environm Genom, Dept Microbiol & Plant Biol, Norman, OK USA
5.Hubei Engn Univ, Sch Life Sci & Technol, Xiaogan, Peoples R China
6.Henan Agr Univ, Coll Life Sci, Zhengzhou, Henan, Peoples R China
Recommended Citation
GB/T 7714
Yu, Dianzhen,Xia, Ming,Zhang, Liping,et al. RpoN (sigma(54)) Is Required for Floc Formation but Not for Extracellular Polysaccharide Biosynthesis in a Floc-Forming Aquincola tertiaricarbonis Strain[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2017,83(14):17.
APA Yu, Dianzhen.,Xia, Ming.,Zhang, Liping.,Song, Yulong.,Duan, You.,...&Qiu, Dongru.(2017).RpoN (sigma(54)) Is Required for Floc Formation but Not for Extracellular Polysaccharide Biosynthesis in a Floc-Forming Aquincola tertiaricarbonis Strain.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,83(14),17.
MLA Yu, Dianzhen,et al."RpoN (sigma(54)) Is Required for Floc Formation but Not for Extracellular Polysaccharide Biosynthesis in a Floc-Forming Aquincola tertiaricarbonis Strain".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 83.14(2017):17.
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