IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Selection of growth-related genes and dominant genotypes in transgenic Yellow River carp Cyprinus carpio L.
Luo, Lifei1,2; Huang, Rong1; Zhang, Aidi1; Yang, Cheng1,2; Chen, Liangming1,2; Zhu, Denghui1,2; Li, Yongming1; He, Libo1; Liao, Lanjie1; Zhu, Zuoyan1; Wang, Yaping1
Corresponding AuthorWang, Yaping(wangyp@ihb.ac.cn)
2018-07-01
Source PublicationFUNCTIONAL & INTEGRATIVE GENOMICS
ISSN1438-793X
Volume18Issue:4Pages:425-437
AbstractTransgenic Yellow River carp is characterized by rapid growth rate and high feed-conversion efficiency and exhibits a great application prospect. However, there is still a significant separation of growth traits in the transgenic Yellow River carp family; as such, growth-related genotypes must be screened for molecular marker-assisted selection. In this study, 23 growth-related candidate genes containing 48 SNP markers were screened through bulked segregant analysis (BSA) among transgenic Yellow River carp family members showing significant separation of growth traits. Then, two growth-related genes (Nos. 17 and 14 genes) were identified through combined genome-wide association study (GWAS) of candidate genes and validation of the full-sibling family approach. Nos. 17 and 14 genes encode BR serine/threonine-protein kinase 2 (BRSK2) and eukaryotic translation-initiation factor 2-alpha kinase 3 (Eif2ak3), respectively. The average body weight of three subgroups carrying the genotypes 17GG, 17GG + 14CC, and 17GG + 14TT of these two genes increased by 27.96, 38.28, and 33.72%, respectively, compared with the controls. The proportion of individuals with body weight > 500 g in these subgroups increased by 19.22, 26.82, and 30.92%, respectively. The results showed that appropriate genotype carriers can be selected from the progeny population through BSA sequencing combined with simplified GWAS analysis. Hence, basic population for breeding can be constructed and transgenic Yellow River carp strains with stable production performance and uniform phenotypic properties can be bred.
KeywordTransgenic Yellow River carp Growth-related genes Genotype Bulked segregant analysis (BSA) Genome-wide association study (GWAS)
DOI10.1007/s10142-018-0597-9
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences
Indexed BySCI ; SCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31672647] ; State Key Laboratory of Freshwater Ecology and Biotechnology[2016FBZ02] ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences[KFJ-STS-QYZD-045]
WOS Research AreaGenetics & Heredity
WOS SubjectGenetics & Heredity
WOS IDWOS:000435577800006
WOS KeywordSTIMULATED INSULIN-SECRETION ; PANCREATIC BETA-CELLS ; COMMON CARP ; HORMONE GENE ; PROVIDES INSIGHTS ; GENOME SEQUENCE ; KINASE ; FISH ; IDENTIFICATION ; POLYMORPHISM
PublisherSPRINGER HEIDELBERG
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/30644
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorWang, Yaping
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Luo, Lifei,Huang, Rong,Zhang, Aidi,et al. Selection of growth-related genes and dominant genotypes in transgenic Yellow River carp Cyprinus carpio L.[J]. FUNCTIONAL & INTEGRATIVE GENOMICS,2018,18(4):425-437.
APA Luo, Lifei.,Huang, Rong.,Zhang, Aidi.,Yang, Cheng.,Chen, Liangming.,...&Wang, Yaping.(2018).Selection of growth-related genes and dominant genotypes in transgenic Yellow River carp Cyprinus carpio L..FUNCTIONAL & INTEGRATIVE GENOMICS,18(4),425-437.
MLA Luo, Lifei,et al."Selection of growth-related genes and dominant genotypes in transgenic Yellow River carp Cyprinus carpio L.".FUNCTIONAL & INTEGRATIVE GENOMICS 18.4(2018):425-437.
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