| Selection of growth-related genes and dominant genotypes in transgenic Yellow River carp Cyprinus carpio L. | |
Luo, Lifei1,2; Huang, Rong1; Zhang, Aidi1; Yang, Cheng1,2; Chen, Liangming1,2; Zhu, Denghui1,2; Li, Yongming1 ; He, Libo1; Liao, Lanjie1; Zhu, Zuoyan1; Wang, Yaping1
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| Corresponding Author | Wang, Yaping(wangyp@ihb.ac.cn) |
| 2018-07-01 | |
| Source Publication | FUNCTIONAL & INTEGRATIVE GENOMICS
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| ISSN | 1438-793X |
| Volume | 18Issue:4Pages:425-437 |
| Abstract | Transgenic Yellow River carp is characterized by rapid growth rate and high feed-conversion efficiency and exhibits a great application prospect. However, there is still a significant separation of growth traits in the transgenic Yellow River carp family; as such, growth-related genotypes must be screened for molecular marker-assisted selection. In this study, 23 growth-related candidate genes containing 48 SNP markers were screened through bulked segregant analysis (BSA) among transgenic Yellow River carp family members showing significant separation of growth traits. Then, two growth-related genes (Nos. 17 and 14 genes) were identified through combined genome-wide association study (GWAS) of candidate genes and validation of the full-sibling family approach. Nos. 17 and 14 genes encode BR serine/threonine-protein kinase 2 (BRSK2) and eukaryotic translation-initiation factor 2-alpha kinase 3 (Eif2ak3), respectively. The average body weight of three subgroups carrying the genotypes 17GG, 17GG + 14CC, and 17GG + 14TT of these two genes increased by 27.96, 38.28, and 33.72%, respectively, compared with the controls. The proportion of individuals with body weight > 500 g in these subgroups increased by 19.22, 26.82, and 30.92%, respectively. The results showed that appropriate genotype carriers can be selected from the progeny population through BSA sequencing combined with simplified GWAS analysis. Hence, basic population for breeding can be constructed and transgenic Yellow River carp strains with stable production performance and uniform phenotypic properties can be bred. |
| Keyword | Transgenic Yellow River carp Growth-related genes Genotype Bulked segregant analysis (BSA) Genome-wide association study (GWAS) |
| DOI | 10.1007/s10142-018-0597-9 |
| Funding Organization | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences |
| Indexed By | SCI ; SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[31672647] ; State Key Laboratory of Freshwater Ecology and Biotechnology[2016FBZ02] ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences[KFJ-STS-QYZD-045] |
| WOS Research Area | Genetics & Heredity |
| WOS Subject | Genetics & Heredity |
| WOS ID | WOS:000435577800006 |
| WOS Keyword | STIMULATED INSULIN-SECRETION ; PANCREATIC BETA-CELLS ; COMMON CARP ; HORMONE GENE ; PROVIDES INSIGHTS ; GENOME SEQUENCE ; KINASE ; FISH ; IDENTIFICATION ; POLYMORPHISM |
| Publisher | SPRINGER HEIDELBERG |
| Funding Organization | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences ; STS regional key program - Bureau of Science and Technology for Development, Chinese Academy of Sciences |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/30644 |
| Collection | 鱼类生物学及渔业生物技术研究中心_期刊论文 |
| Corresponding Author | Wang, Yaping |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| Recommended Citation GB/T 7714 | Luo, Lifei,Huang, Rong,Zhang, Aidi,et al. Selection of growth-related genes and dominant genotypes in transgenic Yellow River carp Cyprinus carpio L.[J]. FUNCTIONAL & INTEGRATIVE GENOMICS,2018,18(4):425-437. |
| APA | Luo, Lifei.,Huang, Rong.,Zhang, Aidi.,Yang, Cheng.,Chen, Liangming.,...&Wang, Yaping.(2018).Selection of growth-related genes and dominant genotypes in transgenic Yellow River carp Cyprinus carpio L..FUNCTIONAL & INTEGRATIVE GENOMICS,18(4),425-437. |
| MLA | Luo, Lifei,et al."Selection of growth-related genes and dominant genotypes in transgenic Yellow River carp Cyprinus carpio L.".FUNCTIONAL & INTEGRATIVE GENOMICS 18.4(2018):425-437. |
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