IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Differential interferon system gene expression profiles in susceptible and resistant gynogenetic clones of gibel carp challenged with herpesvirus CaHV
Mou, Cheng-Yan1,2; Wang, Yang1; Zhang, Qi-Ya1; Gao, Fan-Xiang1,2; Li, Zhi1; Tong, Jin-Feng1,2; Zhou, Li1; Gui, Jian-Fang1
Corresponding AuthorZhou, Li(zhouli@ihb.ac.cn)
2018-09-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume86Pages:52-64
AbstractInterferon (IFN) system plays a vital role in the first line of defense against viruses. In this study, we first identified multiple transcripts of 15 IFN system genes, including PRRs (TLR2, TLR3, RIG-I, and LGP2), PRR-mediated IFN signal pathway (MyD88, MITA, and MAVS), IFN regulatory factors (IRF1, IRF3, IRF7, and IRF9), IFNs (IFN(phi)1 and IFN(phi)3), and ISGs (Mx and viperin), and one transcript of TLR9 in de novo transcriptome assembly data of gibel carp head-kidney. Multiple nucleotide alignments and phylogenetic analysis of common region showed that the transcripts of every of the 15 IFN system genes were classified into two homologs with distinctly divergent sequences, indicating that hexaploid gibel carp may be an allopolyploid. During Carassius auratus herpesvirus (CaHV) infection, gibel carp resistant clone H significantly suppressed CaHV replication with markedly less viral loads than those in highly susceptible clone A(+) and moderately resistant clone F. Then, qPCR analyses were performed to reveal their differential and dynamic expression changes during CaHV infection in head kidney, spleen and liver among three gibel carp gynogenetic clones. Through qPCR and hierarchical clustering analysis, 8 genes, such as RIG-Is, LGP2s, IRF1-B, IRF3s, IRF7s, IRF9-B, Mxs, and viperins, were identified as candidate resistant-related genes. They remarkably increased their expression in immune tissues of three clones after CaHV infection. Significantly, the up-regulation folds of these genes in clone A(+), F and H were related to their resistance ability to CaHV, progressively increasing from susceptible clone to resistant clone at 1 dpi. The positive correlation to the resistance ability suggested that resistant clone H immediately triggered stronger IFN response. IFN(phi)3 showed a different dynamic change and was sharply induced in moderately resistant clone F at 3 dpi. The other 5 IFN system genes (TLR2, TLR3, TLR9, MyD88, and MITA) maintained a low expression level after CaHV challenge. Interestingly, the A or B copies/homologs of almost these IFN system genes exhibited differential transcript abundance in immune tissue after CaHV challenge, suggesting A or B homologs might occur dominant or biased expression of homeologs during gibel carp evolution. These data provide candidate resistant-related genes for disease-resistance breeding of gibel carp. (C) 2018 Elsevier Ltd. All rights reserved.
KeywordGibel carp Herpesvirus Interferon Resistant gynogenetic clone Disease-resistance breeding Resistant-related gene
DOI10.1016/j.dci.2018.04.024
Funding OrganizationStrategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology
Indexed BySCI ; SCI
Language英语
Funding ProjectStrategic Priority Research Program of the Chinese Academy of Sciences[XDA08030202] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA08030201] ; National Natural Science Foundation[131772838] ; earmarked fund for Modern Agro-industry Technology Research System[NYCYTX-49] ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology[2016FBZ01]
WOS Research AreaFisheries ; Immunology ; Zoology
WOS SubjectFisheries ; Immunology ; Zoology
WOS IDWOS:000435060900008
WOS KeywordCARASSIUS-AURATUS-GIBELIO ; SILVER CRUCIAN CARP ; RNA HELICASE LGP2 ; (RIG-I)-LIKE RECEPTORS RLRS ; INNATE IMMUNE-RESPONSE ; RIG-I ; ANTIVIRAL RESPONSE ; CYTOSOLIC DNA ; SUBCELLULAR-LOCALIZATION ; PATHOGEN RECOGNITION
PublisherELSEVIER SCI LTD
Funding OrganizationStrategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology
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Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/30625
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorZhou, Li
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Mou, Cheng-Yan,Wang, Yang,Zhang, Qi-Ya,et al. Differential interferon system gene expression profiles in susceptible and resistant gynogenetic clones of gibel carp challenged with herpesvirus CaHV[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2018,86:52-64.
APA Mou, Cheng-Yan.,Wang, Yang.,Zhang, Qi-Ya.,Gao, Fan-Xiang.,Li, Zhi.,...&Gui, Jian-Fang.(2018).Differential interferon system gene expression profiles in susceptible and resistant gynogenetic clones of gibel carp challenged with herpesvirus CaHV.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,86,52-64.
MLA Mou, Cheng-Yan,et al."Differential interferon system gene expression profiles in susceptible and resistant gynogenetic clones of gibel carp challenged with herpesvirus CaHV".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 86(2018):52-64.
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